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Öğe Determination of the Spiritual Support Perceptions of Students at a Vocational School of Health Services in Turkey(2023) Yıldız Tok, Fatma; Yıldız, İlknurPurpose: The purpose of this study was to determine the frequency of postoperative constipation and risk factors in orthopedic patients. Design: A descriptive model was used. Methods: A total of 140 patients who underwent surgical intervention in the Orthopedics and Traumatology Clinic were included in the present study. The Descriptive Characteristics Form, Constipation Risk Assessment Scale, and Visual Analog Scale were used to collect the study data. Frequencies, percentages, Mann Whitney U, Kruskal-Wallis, and Spearman Correlation tests were used to evaluate the data. Findings: In the present study, 72.1% of the individuals experienced constipation during the hospitalization period, and the constipation risk score was 12.77.á...á4.83. Those who were female, over 65 years old, unemployed, had lower extremity surgery, used opioid analgesics, were dependent on their activities, had prehospital constipation, had a chronic disease, and used drugs regularly had higher constipation risk scores at statistically significant levels. A significant correlation was detected between the constipation risk score and age, pain severity, hospital stay, time to first defecation after surgery, and the amount of fluid consumed daily. Conclusions: The majority of the individuals experienced constipation during the hospitalization period, and the constipation risk score was moderate. Those who were female, had advanced age, were unemployed, had high pain severity, opioid use, previous constipation, chronic disease, regular medication, long hospital stay, those whose first defecation period was prolonged after the surgery and whose fluid intake was insufficient had a higher risk of constipation. In this regard, nurses must evaluate orthopedic patients in terms of constipation risk factors and apply nondrug methods that prevent and relieve constipation with laxatives.Öğe A Common Complication in Orthopedic Patients: Postoperative Constipation and Related Risk Factors(2023) Gürler, Hesna; Yıldız Tok, Fatma; Bekmez, FatmaPurpose: The purpose of this study was to determine the frequency of postoperative constipation and risk factors in orthopedic patients. Design: A descriptive model was used. Methods: A total of 140 patients who underwent surgical intervention in the Orthopedics and Traumatology Clinic were included in the present study. The Descriptive Characteristics Form, Constipation Risk Assessment Scale, and Visual Analog Scale were used to collect the study data. Frequencies, percentages, Mann Whitney U, Kruskal-Wallis, and Spearman Correlation tests were used to evaluate the data. Findings: In the present study, 72.1% of the individuals experienced constipation during the hospitalization period, and the constipation risk score was 12.77.á...á4.83. Those who were female, over 65 years old, unemployed, had lower extremity surgery, used opioid analgesics, were dependent on their activities, had prehospital constipation, had a chronic disease, and used drugs regularly had higher constipation risk scores at statistically significant levels. A significant correlation was detected between the constipation risk score and age, pain severity, hospital stay, time to first defecation after surgery, and the amount of fluid consumed daily. Conclusions: The majority of the individuals experienced constipation during the hospitalization period, and the constipation risk score was moderate. Those who were female, had advanced age, were unemployed, had high pain severity, opioid use, previous constipation, chronic disease, regular medication, long hospital stay, those whose first defecation period was prolonged after the surgery and whose fluid intake was insufficient had a higher risk of constipation. In this regard, nurses must evaluate orthopedic patients in terms of constipation risk factors and apply nondrug methods that prevent and relieve constipation with laxatives.Öğe Green synthesis of reduced graphene oxide and their use on column adsorption of phenol from olive mill wastewater(2023) Haydari, Imane; Aziz, Khalid; Kaya,Savaş; Daştan, Taner; Ouazzani, Naaila; Mandi, Laila; Aziz, FaissalA novel reduced graphene oxide (RGO) synthesis using Verbena officinalis as a green, reducing agent was outreaching. RGO was encapsulated in sodium alginate (SA) by cross-linking (SA-RGO beads). SA-RGO beads have been used to treat olive mill wastewater (OMWW). This effluent is rich in phenolic compounds that inhibit biological degradation and are toxic at high concentrations. The newly prepared SA-RGO beads were characterized by scanning electron microscopy (SEM),energy-dispersive X-ray spectroscopy (EDS), X-ray diffractometer (XRD), BET surface area analyzer, and Fourier transforms infrared spectroscopy (FTIR). In addition, the organic compounds of Verbena officinalis were determined by HPLC to reveal the reduction mechanism of graphene oxide (GO). Finally, batch and fixed-bed column adsorption tests were performed to assess the performance of SA-RGO beads. The kinetics, isotherm models and adsorption thermodynamics show that the pseudo-second-order and Freundlich best present phenol adsorption and the endothermic reaction. Thomas and Yoon-Nelson’s models satisfactorily described fixed-bed column adsorption. The optimized adsorption parameters were 3.68 ... .Öğe Thickness-Dependent Charge Transport in Three Dimensional Ru(II)? Tris(phenanthroline)-Based Molecular Assemblies(2023) Gupta, Ritu; Bhandari, Shapath; Kaya,Savaş; Katin, Konstantin P.; Mondal, Prakash ChandraWe describe here the fabrication of large-area molecular junctions with a configuration of ITO/[Ru(Phen)3]/Al to understand temperature- and thickness-dependent charge transport phenomena. Thanks to the electrochemical technique, thin layers of electroactive ruthenium(II)−tris(phenanthroline) [Ru(Phen)3] with thicknesses of 4−16 nm are covalently grown on sputtering-deposited patterned ITO electrodes. The bias-induced molecular junctions exhibit symmetric current−voltage (j−V) curves, demonstrating highly efficient long-range charge transport and weak attenuation with increased molecular film thickness (β = 0.70 to 0.79 nm−1). Such a lower β value is attributed to the accessibility of Ru(Phen)3 molecular conduction channels to Fermi levels of both the electrodes and a strong electronic coupling at ITO− molecules interfaces. The thinner junctions (d = 3.9 nm) follow charge transport via resonant tunneling, while the thicker junctions (d = 10−16 nm) follow thermally activated (activation energy, Ea ∼ 43 meV) Poole−Frenkel charge conduction, showing a clear “molecular signature” in the nanometric junctions.Öğe Anisotropic Carrier Mobility and Spectral Fingerprints of Two- Dimensional ??Phosphorus Carbide with Antisite Defects(2023) Katin, Konstantin P.; Maslov, Mikhail M.; Nikitenko, Vladimir R.; Kochaev, Alexey I.; Kaya,Savaş; Prezhdo, Oleg V.We apply density functional theory to study carrier mobility in a γ-phosphorus carbide monolayer. Although previous calculations predicted high and anisotropic mobility in this material, we show that the mobility can be significantly influenced by common antisite defects. We demonstrate that at equilibrium concentrations defects do not inhibit carrier mobility up to temperatures of 1000 K. However, defects can change the mobility at high nonequilibrium concentrations of about 10−4 to 10−2 defects per atom. At the low end of this concentration range, defects act as traps for charge carriers and inhibit their mobility. At the high end of this range, defects change the effective carrier masses and deformation potentials, and they can lead to both an increase and a decrease in mobility. We also report the Raman and IR spectra associated with antisite defects. We predict new vibrational modes and shifts of the existing modes due to the defects.Öğe Cyclocondensation of 3,4-diaminobenzophenone with glyoxal: Synthesis, X-ray structure, density functional theory calculation and molecular docking studies(2023) Kaya, Yeliz; Kaya,Savaş; Berisha, Avni; Erçağ, Ayşe7-benzoyl quinoxaline (BQ) was synthesized by the cyclocondensation of 3,4-diaminobenzophenone with glyoxal. BQ was characterized using elemental analysis (C, H, N), FT-IR, 1H NMR and UV–Visible spectral studies. The crystal structure of BQ was solved by single crystal X-ray diffraction method. Chemical reactivity analysis of the newly synthesized molecule was made with the help of well-known quantum chemical descriptors like hardness, chemical potential, first and second electrophilicity indexes and electronic structure rules like Maximum Hardness Principle of Conceptual Density Functional Theory (CDFT). The results of CDFT based calculations showed that this new molecule is stable and can act a good electron acceptor. Molecular docking studies, providing valuable insights into the biological activity of the novel molecule, highlighted its potential to showcase promising therapeutic characteristics against commonly occurring cancer types. This encouragingbehavior will be further validated through upcoming anti-proliferative studies, aiming to confirm its efficacy in combating cancerous cell growth and proliferation.Öğe Adsorption of 40 low molecular weight drugs on pristine and fluorinated C60 fullerenes: Ab initio, statistical and neural networks analysis(2023) Kalika, Elizaveta B.; Bondarev, Nikolay V.; Katin, Konstantin P.; Kochaev, Alexey I.; Grekova, Anastasiya A.; Kaya,Savaş; Bauetdinov, Yusupbek A.; Maslov, Mikhail M.Loading of 40 small drugs molecules with molecular weights in 47–210 g/mol range consisted of H, C, N, O, F, S elements on C60, C60F2 and C60F48 fullerenes are studied with the density functional theory. Adsorption energies as well as deformation energies of fullerenes and drugs are calculated. The C60F2 is recognized as the most active drugs adsorbent. Mean adsorption energies averaged over all considered drugs are 0.41, 0.59 and 0.54 eV with standard deviations of 0.10, 0.09 and 0.14 eV for C60, C60F2 and C60F48 fullerenes, respectively. Mean energies of drug-induced deformations of C60, C60F2 and C60F48 are 8.6, 12.0 and 16.6 meV, respectively. Multilinear regressions and neural networks were proposed for prediction of adsorption energies based on dipole moments and frontier molecular orbitals of the drugs. Achievable accuracy of such predictions is about 0.1 eV. Altretamine antibiotic is recognized as the notable drug possessing extremely high binding energies about 1 eV with fullerenes. Infrared spectral shifts due to its loading on fullerene carriers are calculated.Öğe Copper(II) chelates derived from an N,N,O-tridentate 2-pyridinecarboxaldehyde-N 4 -phenylsemicarbazone: Synthesis, spectral aspects, crystal structure, FMO and NBO analysis(2023) Sithambaresan, M.; Kurup, M. R. Prathapachandra; Serdaroğlu, Goncagül; Kaya,SavaşSeven novel Cu(II) complexes [Cu(HPySc)Cl 2 ] ( 1 ), [Cu 2 (PySc) 2 (NO 3 ) 2 ] ·H 2 O ( 2 ), [Cu(HPySc)(SO 4 )] ·½H 2 O ( 3 ), [Cu 2 (PySc) 2 (OAc) 2 ] ·CH 3 OH ( 4 ), [Cu 2 (PySc) 2 Br 2 ] ·H 2 O ( 5 ), [Cu(PySc)(NCS)] ·2H 2 O ( 6 ) and [Cu 2 (PySc) 2 (N 3 ) 2 ] ( 7 ) with 2-pyridinecarboxaldehyde-N 4 -phenylsemicarbazone (HPySc) have been prepared and character- ized using different analytical and spectroscopic techniques. The complex 6 is expected to be square planar while the other complexes are expected to be square pyramidal arrangement, among these, the geometry of complex 4 has been conformed by single crystal XRD study that it is a dimer and adopts distorted square pyramidal geometry around copper(II) centre. All the complexes are found to be para- magnetic and non-conductive in nature. The semicarbazone ligand is coordinated in neutral form in two of the complexes and in ionic form in others. DFT/B3LYP/6-311g ∗∗/LANL2DZ computations of the ligand HpySc (PyCHNNCONHPh) and seven Cu(II) complexes were performed to analyze the FMOs and impor- tant electron delocalizations that existed in each compound. Molecular stability and bond strengths have been investigated by applying natural bond orbital (NBO) analysis. All the complexes are more stable than the semicarbazone and the complex 2 is the most electronically stable (-5.160 eV) among the com- plexes. Furthermore, the semicarbazone is the hardest and complex 5 is the softest than all complexes due to the ηvalues. The calculated energy gap between HOMO and LUMO energies show the variations of nucleophilic and electrophilic reactivity regions in the semicarbazone and in complexes 1-7 .Öğe Biofabrication of copper oxide nanoparticles using Solanum tuberosum L. var. Vitelotte: characterization, antioxidant and antimicrobial activity(Springer, 2023) Keskin, Zehra Seba; Topal Canbaz, Gamze; Yokuş, Ahmet; Aslan, RukiyeIn this study, an efective and inexpensive PAA-CG composite was synthesized using polyacrylamide (PAA) and cofee ground (CG) with the help of the in situ polymerization method. The adsorption efciency of this new composite was investigated in the removal of Pb(II) ions from an aqueous solution. Characterization was done by Fourier transform infrared spectroscopy, scanning electron microscopy, energydispersive spectroscopy and zero charge point analysis. The maximum adsorption capacity of the PAA-CG composite was calculated as 0.622 mol/kg according to the Langmuir isotherm model. From the obtained data, it was determined that the adsorption mechanism is compatible with pseudo-second-order kinetics and intraparticle difusion model. The calculated thermodynamic data showed that adsorption is endothermic, spontaneous and entropy increasing.Öğe In silico comparative transcriptome analysis of Papaver somniferum cultivars(22.12.2023) Gürkök Tan, Tuğba; Türktaş, Mine; Güçlü,GülşenPapaver somniferum is a medicinal plant of the Papaveraceae family that has traditionally been used for diet or its therapeutic value for thousands of years. Mainly, morphine and noscapine alkaloids exhibit anti-analgesic and anti-cancer effects. However, gene expression patterns and regulatory elements, such as transcription factors between different tissues, still need to be detected. In this study, comparative in silico transcriptome analyses were conducted to examine the tissue-specificity of the benzylisoquinoline alkaloids (BIAs) biosynthetic genes and transcription factors (TFs) between morphine and noscapine cultivars. Analysis showed that BIA biosynthetic genes are expressed in a different pattern between two varieties. Results showed that some members of plant-specific secondary metabolites related to TF families, such as MYB, MADS-box, bHLH, NAC, and WRKY, are differentially expressed between tissues and varieties.Öğe The influence of laughter yoga on pregnancy symptoms, mental well-being, and prenatal attachment: A randomized controlled study(Taylor & Francis, 10.01.2023) Ağapınar Şahin, Safiye; Bekar, MineThe researchers’ aim was to evaluate the impact of laughter yoga on pregnancy symptoms, mental well-being, and pre-natal attachment. They carried out this study on 85 pregnant women randomized in a maternity hospital in the Eastern Anatolia Region of Turkey. The researchers collected data using the Pregnant Introduction Form, Pregnancy Symptom Inventory (PSI), Warwick-Edinburgh Mental Well-being Scale (WEMWBS), and Prenatal Attachment Inventory (PAI). After applying the laughter yoga practice, they found that mental well-being and prenatal attachment levels were higher in pregnant women in the experimental group and the difference was statistically significant. Thus, they concluded that laughter yoga was an effective practice in reducing the frequency of pregnancy symptoms and limiting daily activities by preg-nancy symptoms and increasing mental well-being and pre-natal attachment levels.Öğe From the perspective of Turkish women: intimate partner violence and perceived stress level in the Covid-19 pandemic(2022) Evcili, FundaPandemic process increase the vulnerability of women to intimate partner violence (IPV). IPV is one of the greatest stress sources a woman can face. This study’s purpose to determine effects of the Covid-19 pandemic process on the IPV status and perceived stress levels of women. The population of the descriptive study consisted 834 women working at a state university in Turkey’s. 452 women who with intimate partner relationships were included in the sample. Data collection tools were applied via online survey link. The mean age of the women was 30.6 ± 4.20. The The Perceived Stress Scale mean scores of the women were 40.18 ± 3.20. They are not able to cope with stress effectively. It was determined that women participated the study were exposed to more IPV during the pandemic process. It was found that as the frequency of IPV increased, women’s perception of stress also increased. Research such as this can be used to help inform decision-makers as they grapple with the adverse negative effects of public health safety measures related to Covid-19.Öğe The effects of workload on the styles of anger expression and “trait anger” of healthcare professionals working in a COVID?19 pandemic hospital(2022) Evcili, FundaAbstract Purpose: The aim of the study was to determine the effects of workload on the styles of anger expression and “trait anger” of health professionals working in a COVID‐19 pandemic hospital. Design and Methods: This descriptive study was carried out at a COVID‐19 pandemic hospital in Turkey. The sampling consisted of 493 healthcare professionals. The data were evaluated with the SPSS (23.0) software program. Findings: The study found that the participants who perceived the workload as higher, and could not tolerate the work they performed, were high in “trait anger” levels and their anger was suppressed. Practice Implications: Health institutions should consider the recognition of emotional risks and the planning of interventions as a priority for professionals involved in the care of COVID‐19 infected patients. KEYWORDS: anger, COVID‐19, health professional, pandemic, t‐anger, workloadÖğe A Preliminary Molecular Study on the Haplotypes of Varroa destructor in Turkey(15.06.2022) Doğan, ÖzgülVarroosis is one of the most important diseases of honeybees. Four species, Varroa jacobsoni, Varroa destructor, Varroa underwoodi and Varroa rindereri are present in the genus of Varroa, the most prevalent one being V. destructor. V. destructor has different haplotypes which have different pathogenic effects on the honeybees. Korean (K) haplotype is more prevalent and pathogenic than the Japanese ( J) haplotype. The aim of this study was to determine the haplotypes of V. destructor in Turkey. Female mites from ten different provinces of Turkey were examined. After DNA extraction partial sequences of mitochondrial COX1 gene was amplified with PCR and subsequently sequenced. At the conclusion of molecular and phylogenetic analysis, we determined that all isolates were identical to each other and corresponded with the K haplotype of V. destructor, with 100% identity. This study supports preliminary data regarding the haplotypes of V. destructor from Ankara, Cankiri, Corum, Elazig, Kirsehir, Kirikkale, Hakkari, Hatay, Samsun and Tekirdag provinces of Turkey. Considering that Turkey is a bridge between Asia, Europe and Africa, more detailed studies should be conducted for the determination of different haplotypes or variants of V. destructor.Öğe Phylogenomic Analyses of the Tenthredinoidea Support the Familial Rank of Athaliidae (Insecta, Tenthredinoidea)(MDPI, 21.09.2022) Doğan, ÖzgülThe systematic status of the genus Athalia and related genera is a perennial controversy in sawfly taxonomy. Several authors have hypothesized that the placement of Athalia within the Tenthredinidae is artificial, but no studies have focused on this topic. If the hypothesis that Athalia does not belong to Tenthredinidae can be supported, the taxonomic framework of Tenthredinoidea needs revision. We present a comprehensive phylogenomic study of Tenthredinoidae, focusing on the positions of Athalia and related genera by sampling 80 representatives mainly of the Tenthredinoidea, including Heptamelinae and Blasticotomidae. Our phylogenetic reconstructions based on nuclear genes and mitochondrial (mt) sequences support Athalia and related genera as a distinct clade sister to Tenthredinidae + (Cimbicidae + Diprionidae). A comparison of symphytan mitochondrial genomes reveals an innovative gene rearrangement pattern in Athaliidae, in which Dentathalia demonstrates a more ancestral pattern than Athalia and Hypsathalia. The lineage specificity of mt rRNA secondary structures also provides sufficient support to consider Athaliidae as a separate family. In summary, the phylogeny and genomic structural changes unanimously support the taxonomic treatment of Athaliidae as a family and the re-establishment of Dentathalia as a valid genus.Öğe Pulmonate slug evolution is reflected in the de novo genome of Arion vulgaris Moquin-Tandon, 1855(Nature Research, 20.08.2022) Doğan, ÖzgülStylommatophoran pulmonate land slugs and snails successfully completed the water‑to‑land transition from an aquatic ancestor and flourished on land. Of the 30,000 estimated species, very few genomes have so far been published. Here, we assembled and characterized a chromosome‑level genome of the “Spanish” slug, Arion vulgaris Moquin‑Tandon, 1855, a notorious pest land slug in Europe. Using this reference genome, we conclude that a whole‑genome duplication event occurred approximately 93–109 Mya at the base of Stylommatophora and might have promoted land invasion and adaptive radiation. Comparative genomic analyses reveal that genes related to the development of kidney, blood vessels, muscle, and nervous systems had expanded in the last common ancestor of land pulmonates, likely an evolutionary response to the terrestrial challenges of gravity and water loss. Analyses of A. vulgaris gene families and positively selected genes show the slug has evolved a stronger ability to counteract the greater threats of external damage, radiation, and water loss lacking a protective shell. Furthermore, a recent burst of long interspersed elements in the genome of A. vulgaris might affect gene regulation and contribute to rapid phenotype changes in A. vulgaris, which might be conducive to its rapid adaptation and invasiveness.Öğe The complete mitochondrial genome of Syrista parreyssii (Spinola, 1843) (Hymenoptera: Cephidae) and its phylogenetic analyses(Taylor&Francis, 28.09.2022) Doğan, ÖzgülThe complete mitochondrial genome ofSyrista parreyssii(Spinola,1843) was described. The circulargenome is 18,666bp with an AþT content of 82.60%. It contains 37 genes and a 1921 bp controlregion. The CR-trnI(þ)-trnQ(–)-trnM(þ) cluster is rearranged astrnM(þ)-CR-trnQ(–)-trnI(þ) cluster.Phylogenetic analysis demonstrates that EuropeanSyristaand AsianNeosyristawere not sister groups.Neosyristais a valid genus and should be reestablished. Moreover, a preliminary study based on COIshowed there are at least three validSyristaspecies within the European and Mediterranean regions.Whether the knownSyrista parreyssii(Spinola,1843) is a complex or there are more cryptic speciesneeds further study.Öğe Characterization and taxonomic utility of ITS2 in Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae)(ENTOMOLOGICAL SOC TURKEY, EGE UNIV, 05.06.2022) BUDAK, MAHİR; KORKMAZ, ERTAN MAHİR; HASTAOĞLU ÖRGEN, SEVDA; BAŞIBÜYÜK, HASAN HÜSEYİNThe widespread use of ITS2 as a potential marker in insects has accelerated species-level phylogenetic studies. Reliable and quality data can be obtained thanks to the features such as rapid evolution and secondary structure of this marker. This paper presents the phylogenetic relationship among 36 individuals of Dolerus Panzer, 1801 (Hymenoptera: Tenthredinidae) collected between 2002 and 2018 to obtain the first data on ITS2 secondary structure. Aligned of ITS2 data were analyzed by application of maximum likelihood method to reveal phylogenetic relationship among the specimens. Also, the structural properties, length variation and presence of compensatory base changes make the ITS2 useful marker in determination of species boundaries of closely related species. Four species (Dolerus triplicatus (Klug, 1818), Dolerus germanicus (Fabricius, 1775), Dolerus puncticollis Thomson, 1871 and Dolerus nigratus (M??ller, 1776) and two putative species (Dolerus spp. 1 and 2) were determined from 36 individuals belonging to Dolerus.Öğe Synthesis, characterization, and application of polyacrylamide/carmine polymer nanomaterial as an effective solid-phase material for ultrasonic-assisted solid-phase microextraction of aluminum and chromium in vegetable samples(Springer, 2022) Ulusoy, Songül; Elik Adil; Ulusoy, Halil İbrahim; Şimşek, Selçuk; Altunay NailHere, a new and inexpensive polymer nanomaterial consisting of polyacrylamide and carmine (PAM-CR) was synthesized to provide selective extraction of aluminum and chromium from vegetable samples prior to their determination by flame atomic absorption spectrometry. Various characterized techniques including scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray powder diffraction were applied to investigate the structure and morphology of the PAM-CR. Important factors were investigated and optimized to improve the effectiveness of the adsorbent. Under optimized conditions, calibration graphs for Al(III) and Cr(III) were linear in the range from 0.2 to 400.0 μg L− 1 and 1.0 to 500.0 μg L− 1, respectively. Satisfied recoveries between 93.1% and 102.7% were obtained with detection limits of 0.06 μg L−1 and 0.35 μg L−1 for Al(III) and Cr(III), respectively. The relative standard deviation was in range of 2.0–2.3%. The PAM-CR can be reused 8 times without significant loss in the recovery. After validation study with certified reference material, the proposed method was successfully applied to the pre-concentration and determination of Al(III) and Cr(III) in vegetable samplesÖğe Application of cloud point extraction for residues of chloramphenicol and amoxicillin in milk samples by HPLC–DAD(Springer, 2022) Sürücü, Bilge; Ulusoy, Halil İbrahim; Ulusoy, Songül; Demir, Özge; Gülle, SümeyraA pre-concentration and sensitive determination method for residues of Chloramphenicol (CLP) and Amoxicillin (AMX)in milk samples were developed based on cloud point extraction (CPE) and HPLC–DAD analysis. CLP and AMX moleculeswere extracted to surfactant phases of polyethylene glycol-6000 (PEG-6000) in the presence of pH 7.0 buffer andhigh electrolyte concentration. Experimental variables were examined and optimized such as concentration of electrolyteand surfactant, pH, incubation time, and diluting solvent. In the developed method, determination of CLP and AMX usingHPLC system was carried out by isocratic elution of water:acetonitrile (30:70) mixture. CLP and AMX antibiotic molecules were analyzed by considering peak area obtained by DAD detector at 273 nm and 276 nm, respectively. After optimizationexperimental variables, analytical signals were linear in the range of 10–900 ng mL− 1 and 25–1000 ng mL−1, respectively.The limit of detection values was calculated as 2.98 and 7.46 ng mL−1 while relative standard deviations (RSD %) were lowerthan 4.20% for 100 ng mL−1. Finally, the developed method was successively applied to cow milk samples.
