Tuzcu, MehmetTuzcu, NevinBaşbuğ, Onur2024-10-262024-10-2620201309-69582146-1953https://doi.org/10.15312/EurasianJVetSci.2020.316https://search.trdizin.gov.tr/tr/yayin/detay/413559https://hdl.handle.net/20.500.12418/23249Aim: In cattle breeding, pneumonia caused by Pasteurella multocida andMannheimia haemolytica is very important due to the economic losses caused. In this study, it was aimed to reveal cytological findings in pneumonicbovine lungs and nasal swaps due to P. multocida and M. haemolytica determined by microbiological methods and real time PCR.Materials and Methods: In this study, bacteriological culture, histopathological, cytological and real time PCR techniques were used in pneumonic bovine lungs. Cytological findings were evaluated in cases of pneumonia whoseetiological agent was identified as M.haemolytica and P.multocida by realtime PCR and were compared with other pneumonia typesResults: Cytological findings were recorded in smear samples prepared fromnasal swaps and lung samples. Pneumonia caused by P. multocida and M.haemolytica was detected in 23 cases (9.87%) out of 233 cattle with signsof pneumonia. P.multocida and M.haemolytica were determined to occur inabout 51.11% of the animals with fibrinous pneumonia. During the cytological examination of these cases, neutrophils were seen increased in numbercompared to the other types of pneumonia. In addition to neutrophils, thenumber of lymphocytes and ciliated epithelial cells was also significantly increased in these cases compared to the other fibrinous pneumonia cases inwhich P.multocida ve M.haemolytica was not detected.Conclusion: The results of this study showed that P. multocida and M. haemolytica cause a fibrinous type of pneumonia and constitute an importantportion of cattle respiratory diseases. Clinically, cytology results may be evaluated for the typing of pneumonia.en10.15312/EurasianJVetSci.2020.316info:eu-repo/semantics/openAccessPathological, cytological, microbiological and molecular investigations of pneumonia caused by Pasteurella multocida and Mannheimia haemolyticaArticle364339331413559