Tastemur, SeymaHacisuleyman, LeventKarata, OzhanYulak, FatihAtaseven, Hilmi2024-10-262024-10-2620230008-42121205-7541https://doi.org/10.1139/cjpp-2022-0523https://hdl.handle.net/20.500.12418/29719Colorectal cancer is the third most lethal and fourth most commonly diagnosed cancer worldwide. Sinapic acid, a derivative of hydroxycinnamic acid, is a promising phytochemical exhibiting numerous pharmacological activities in various systems. It is a substantial chain-breaking antioxidant that operates as a radical scavenger. The aim of this research was to investigate the antiproliferative effect of sinapic acid on the HT-29 cell line besides the mechanisms underlying this activity. The effect of sinapic acid on the viability of HT-29 cell line was investigated using XTT assay. The levels of BCL-2, cleaved caspase 3, BAX, cleaved PARP, and 8-oxo-dG were measured using ELISA. Gamma-H2AX and cytochrome c expressions were assessed semiquantitatively using immunofluorescence staining. Sinapic acid at 200 & mu;m and higher doses produced a significant antiproliferative effect on HT-29 cells. The IC50 value was found to be 317.5 & mu;m for 24 h. Sinapic acid (317.5 & mu;m) significantly elevated cleaved caspase 3, BAX, cleaved PARP, and 8-oxo-dG levels. The levels of gamma-H2AX foci are significantly higher, while the levels of cytochrome c are lower in sinapic acid-treated HT-29 cells. These results indicate that sinapic acid has antiproliferative, apoptotic, and genotoxic effects on colon cancer cells.en10.1139/cjpp-2022-0523info:eu-repo/semantics/closedAccesssinapic acidcolorectal cancerHT-29 cell lineantiproliferative effectArticle101736836136996488WOS:001024358700004Q3