Bayyurt, BurcuArslan, Serdal2025-05-042025-05-0420252619-8991https://doi.org/10.34248/bsengineering.1578992https://search.trdizin.gov.tr/tr/yayin/detay/1295867https://hdl.handle.net/20.500.12418/34848Oxidized low-density lipoprotein (ox-LDL) shows many harmful effects such as induction of apoptosis on function of endothelial cell (EC). Fas ligand (FASL) induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells (HUVECs) with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction (qPCR). We compared FASL gene expression level between the groups according to ∆∆Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL (P<0.001). We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.en10.34248/bsengineering.1578992info:eu-repo/semantics/openAccessFas ligandGene expressionHuman umbilical vein endothelial cellOxidized low-density lipoproteinQuantitative polymerase chain reactionArticle8157541295867