Cevik, Ulviye AcarIsik, AysenKapavarapu, RavikumarKucukoglu, KaanNadaroglu, HayrunnisaBostanci, Hayrani ErenOzkay, Yusuf2025-05-042025-05-0420240022-28601872-8014https://doi.org/10.1016/j.molstruc.2023.136770https://hdl.handle.net/20.500.12418/35491In this study, we synthesized a series of new benzimidazole-triazole (6a-6k) derivatives and characterized them by 1H NMR, 13C NMR, and HRMS. These compounds were evaluated for their inhibitory activity against hCA-I and hCA-II. All the compounds exhibited good hCA-I and hCA-II inhibitory activities with IC50 values in the range of 1.158 mu M to 3.48 mu M. Among all these compounds, compound 6j, with an IC50 value of 1.288 mu M and 1.6197 mu M, is the most active against hCA-I and hCA-II, respectively. Compounds 6a-6k were also evaluated for their cytotoxic effects on the L929 mouse fibroblast (normal) cell line. Enzyme inhibition kinetics showed all compounds 6a-6k to inhibit the enzyme by non-competitive. The most active compound 6j was subjected to molecular docking, which revealed their binding interactions with the enzyme's active site, confirming the experimental findings.en10.1016/j.molstruc.2023.136770info:eu-repo/semantics/closedAccessBenzimidazole4-TriazoleCarbonic anhydraseMolecular dockingCytotoxicityArticle12952-s2.0-85173844631Q1WOS:001097119900001Q2