Dural, Emrah2024-10-262024-10-2620201386-20731875-5402https://doi.org/10.2174/1386207323666200630113850https://hdl.handle.net/20.500.12418/28154Aim and Scope: Due to the serious toxicological risks and their widespread use, quantitative determination of phthalates in cosmetic products have importance for public health. The aim of this study was to develop a validated simple, rapid and reliable high-performance liquid chromatography (HPLC) method for the determination of phthalates which are; dimethyl phthalate (DMP), diethyl phthalate (DEP), benzyl butyl phthalate (BBP), di-n-butyl phthalate (DBP), di(2-ethylhexyl) phthalate (DEHP), in cosmetic products and to investigate these phthalate (PHT) levels in 48 cosmetic products marketing in Sivas, Turkey. Materials and Methods: Separation was achieved by a reverse-phase ACE-5 C-18 column (4.6 x 250 mm, 5.0 mu m). As the mobile phase, 5 mM KH2PO4 and acetonitrile were used gradiently at 1.5 ml min(-1). All PHT esters were detected at 230 nm and the run time was taking 21 minutes. Results: This method showed the high sensitivity value the limit of quantification (LOQ) values for which are below 0.64 mu g mL(-1) of all phthalates. Method linearity was >= 0.999 (r(2)). Accuracy and precision values of all phthalates were calculated between (-6.5) and 6.6 (RE%) and <= 6.2 (RSD%), respectively. Average recovery was between 94.8% and 99.6%. Forty-eight samples used for both babies and adults were successfully analyzed by the developed method. Results have shown that, DMP (340.7 mu g mL(-1) +/- 323.7), DEP (1852.1 mu g mL(-1) +/- 2192.0), and DBP (691.3 mu g mL(-1) +/- 1378.5) were used highly in nail polish, fragrance and cream products, respectively. Conclusion: Phthalate esters, which are mostly detected in the content of fragrance, cream and nail polish products and our research in general, are DEP (1852.1 mu g mL(-1) +/- 2192.0), DBP (691.3 mu g mL(-1) +/- 1378.5) and DMP (340.7 mu g mL(-1) +/- 323.7), respectively. Phthalates were found in the content of all 48 cosmetic products examined, and the most detected phthalates in general average were DEP (581.7 mu g mL(-1) +/- 1405.2) with a rate of 79.2%. The unexpectedly high phthalate content in the examined cosmetic products revealed a great risk of these products on human health. The developed method is a simple, sensitive, reliable and economical alternative for the determination of phthalates in the content of cosmetic products, it can be used to identify phthalate esters in different products after some modifications.en10.2174/1386207323666200630113850info:eu-repo/semantics/closedAccessPhthalatesDEPDMPBBPDBPDEHPcosmeticsHPLC-UVmethod validationArticle231010221010326032772-s2.0-85096658673Q3WOS:000604264700004Q3