Kirici, MuammerTuzun, BurakKirici, MahinurAtamanalp, MuhammedPoustforoosh, AlirezaBeydemir, SukruTaysi, Mehmet Resit2024-10-262024-10-2620240167-73221873-3166https://doi.org/10.1016/j.molliq.2024.124288https://hdl.handle.net/20.500.12418/30429The first enzyme of the pentose phosphate metabolic pathway is glucose 6 -phosphate dehydrogenase (d -glucose6 -phosphate: NADP + oxidoreductase EC1.1.1.49; G6PD). G6PD has essential functions such as membrane lipid synthesis, ribose 5 -phosphate, and NADPH production. In this study, the G6PD enzyme was purified from kidney, liver, and gill tissues of Capoeta trutta, one of the dominant fish species in the Euphrates -Tigris River System, and the in vitro effects of some metals (Ag+, Cd2+, Cu2+, Fe2+, Ni2+, Pb2+ and Zn2+) on the enzyme activity were investigated. For this purpose, firstly, the G6PD enzyme was purified from tissues using a 2 ', 5 '-ADP Sepharose 4B affinity column. The purity of the enzyme was checked by the SDS-PAGE method and a single band was seen in the gel. After the purity of the enzyme was determined, the effects of metals on the enzyme activity were determined using the spectrophotometric method. As a result of the study, it was determined that the Ag+ ion was the most potent inhibitor for C. trutta gill, kidney, and liver G6PD enzymes. Lastly, calculations were made to examine the activity of the glucose 6 -phosphate dehydrogenase molecule against the G6PD enzymes. Afterwards, the interaction of the glucose 6 -phosphate dehydrogenase molecule with the protein (PDB ID: 5JYU and 2BH9) with the highest activity was calculated in the range of 0-100 ns. Finally, ADME/T calculation was made to predict the effects and reactions of glucose 6 -phosphate dehydrogenase molecule in human metabolism. This study explores the physiological functions and environmental sensitivities of G6PD in a dominant fish species, while also investigating its potential interactions and metabolic roles in humans. Understanding these aspects can contribute to environmental monitoring, fish health management, and even pharmaceutical development.en10.1016/j.molliq.2024.124288info:eu-repo/semantics/closedAccessCapoeta truttaGlucose 6-phosphate dehydrogenaseInhibitionMolecular dockingMolecular dynamicArticle3992-s2.0-85186526236Q1WOS:001204476700001N/A