Hepokur, CeylanMisir, SemaTunc, TutkuTutar, UgurHepokur, Ali IhsanCicek, Mehmet2024-10-262024-10-2620200250-46851303-829Xhttps://doi.org/10.1515/tjb-2019-0470https://search.trdizin.gov.tr/tr/yayin/detay/455043https://hdl.handle.net/20.500.12418/27635Objectives: In this study, we aimed to detect the chemical compounds of Thymbra capitata ethanolic extract (TC-EtOH) as well as to evaluated its antimicrobial, antioxidant, cytotoxic activities, and in vivo wound healing effects. Methods: The chemical composition of TC-EtOH was analyzed by Gas chromatography-mass spectrometry (GC-MS). Antioxidant and antimicrobial properties were determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH), disc diffusion test and broth micro-dilution (minimal inhibitory concentration [MIC]) methods, respectively. Cytotoxic activity was tested on MG63 (human osteosarcoma) and MCF-7 (human breast carcinoma) cells by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. Tumor necrosis factor alpha (TNF-alpha) protein levels were determined by ELISA. Results: The major components of TC-EtOH were tetratria contane (14.92%), camphor (12.50%), and terpineol (10.77%). TC-EtOH showed powerful antimicrobial activity in C. Tropicalis (0.03 mg/mL). The IC50 values of the TC-EtOH of the DPPH were determined 21.5 mu g/mL. The IC50 values were calculated 37.28 and 44.40 mu g/mL on the MG63 and MCF-7 cell lines, respectively. It was observed that the wounds treated with TC-EtOH showed a faster healing. Conclusions: According to results, T. capitata species are thought to be natural antioxidants and a novel pharmaceutical compound for the pharmaceutical industry.en10.1515/tjb-2019-0470info:eu-repo/semantics/openAccessantimicrobialantioxidantcytotoxic activityThymbra capitatawound healingArticle4568498432-s2.0-85098958602Q4455043WOS:000603517600025Q4