Temel Eczacılık Bilimleri Bölümü Bildiri / Sunu / Poster Koleksiyonu
https://hdl.handle.net/20.500.12418/438
2024-03-29T14:01:58ZThe Effect of Vitamins on Oxidation in Experimentally Diabetes Conditioned Rats
https://hdl.handle.net/20.500.12418/14902
The Effect of Vitamins on Oxidation in Experimentally Diabetes Conditioned Rats
Diabetes Mellitus (DM) is a metabolic disorder that is very
common in our society, is caused by a combination of hereditary
and environmental factors, and results in an increase in blood
glucose levels. Diabetes mellitus develops as a result of increased
blood glucose level and impaired insulin secretion. Antioxidant
enzymes cannot be synthesized enough in patients with diabetes
mellitus and free radicals damage tissues. In this study, the effects of vitamin C and vitamin E on lipid
peroxidation and antioxidant enzyme activities in kidney tissue
of streptozotocin-induced diabetic rats were investigated. Male
wistar rats, were grouped into five each consisting 8 rats as (nondiabetic
control
(K),
diabetic
(D),
diabetic
Vitamin
E (E),
diabetic
Vitamin
C (C),
and
diabetic
Vitamin
E and
C (EC).
Diabetes
mellitus
was
induced
in rats
by
intraperitoneal
injection
of 55mg/
kg
STZ.
After
the injection,
i.p.
268mg/kg.
vitamin
E, 250 mg/
kg
vitamin
C by
gavage
were
administrated
for four weeks.
As
an
indicator
of lipid
peroxidation
malondialdehyde
(MDA)
levels,
antioxidant
enzymes
as superoxide
dismutase
(SOD),
catalase
(CAT)
and
and
glutothione
peroxides
(GSH-PX)
activities
were
measured
in kidney
tissue
homogenates.
In the study, MDA level of group D was significantly higher
than groups K, E, C and EC. Statistically significant difference
was not observed in CAT levels among the groups. SOD enzyme
levels in group D was higher compared to group K, and lower in
group EC compared to groups C, and D. GSH-Px enzyme levels
in group D and group C were significantly higher compared to
group K. Group D GSH-Px levels were significantly lower whwn
compared to group E, C and EC, GSH-Px levels of EC group was
lower compared to group C.
As a result, in this study it was demonstrated that vitamin C
and vitamin E have positive effect on lipid peoxidation and as a
result decreased the high levels of MDA, SOD, GSH-Px levels of
diabetic rat kidney tissues.
0027-01-01T00:00:00ZInvestigation of the Relationship between the Chemical Structures and the Cytotoxicity of Some Maleic Anhydride-Containing Copolymers as Polymeric Drug Carriers
https://hdl.handle.net/20.500.12418/14901
Investigation of the Relationship between the Chemical Structures and the Cytotoxicity of Some Maleic Anhydride-Containing Copolymers as Polymeric Drug Carriers
Polymer-based substances have been an important biomaterial in the advancement of drug
delivery systems as they enabled the controlled release of therapeutic agents in fixed doses with high
bioavailability, lower toxicity, and higher pharmaceutical efficacy. This study aimed to find out whether
there is a relationship between cytotoxicity and the chemical structure of the copolymers that act as
carriers in many copolymer-drug conjugates by carrying out their characterization using spectroscopy
and morphology-elucidated techniques described in our previous studies. Selected maleic anhydride,
including polymeric carriers such as poly(maleic anhydride-co-methyl methacrylate) (MAMMA),
poly(maleic anhydride-alt-vinyl acetate) (MAVA), and poly(maleic anhydride-alt-allylphenyl ether
(MAAPE) were synthesized via a charge transfer complex (CTC) produced by a radical chain
copolymerization reaction.
1,2,3
In addition, the commercial form of poly(4-styrenesulfonic acid-comaleic
acid) (PSSMA) was purchased. The cytotoxic effects of all samples were examined using MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) analysis for L929 (fibroblast cell), MDA
(breast cancer), MCF7 (breast cancer), and C6 cell lines (glial tumor) were examined in vitro by
comparison with cisplatin (reference compound). The Annexin V binding assay was also studied for all
copolymers. When compared with cisplatin, which is actively used in the clinic for cancer therapy,
PSSMA was found to have a cytotoxic effect on C6 cells, although not as strong as cisplatin, but the
most effective copolymer was MAMMA due to its low IC50 value. In summary, the pharmaceutical
agents delivered with these carriers could represent a promising and effective therapeutic modality
for glioblastoma.
0023-01-01T00:00:00ZINVESTIGATION OF SALVIA CADMICA’S ANTIOXIDANT PROPERTIES AND CYTOTOXIC PROPERTIES IN BREAST CANCER CELL LINE AND ITS APOPTOTIC INDUCTION
https://hdl.handle.net/20.500.12418/14900
INVESTIGATION OF SALVIA CADMICA’S ANTIOXIDANT PROPERTIES AND CYTOTOXIC PROPERTIES IN BREAST CANCER CELL LINE AND ITS APOPTOTIC INDUCTION
Salvia cadmica is an endemic species known to have antioxidant, antimicrobial and aromatic
properties and the existence of Salvia species is known to date back to 753 BC. This species,
whose medicinal properties were first discovered by the Romans at this time, is used in a wide
variety of fields. Salvia species, which have a content especially rich in essential oils, are often
used in cosmetic, medical, and industrial fields. In addition, it has been shown by past studies
that the root parts of salvia species contain terpenoids, polyphenols and essential fatty acids.
Numerous cancer studies have been conducted with salvia species, and it seems that not enough
research has been done for salvia cadmica. In this study, IC50 values for Total Antioxidant
Status (TAS) were determined by ethanol extract taken from the above-ground parts of Salvia
cadmica. Then, the cytotoxicity in the breast cancer cell line (MCF-7) and healthy fibroblast
cell line was determined by using the MTT method. Accordingly, the IC50 dose of Salvia
Cadmica was higher than 78.13 ± 4.82 μg/mL for the MCF-7 cell line.
0030-01-01T00:00:00ZINVESTIGATION OF THE ANTIPROLIFERATIVE EFFECT OF SALVIA CADMICA ON COLON CANCER
https://hdl.handle.net/20.500.12418/14898
INVESTIGATION OF THE ANTIPROLIFERATIVE EFFECT OF SALVIA CADMICA ON COLON CANCER
Salvia cadmica is an endemic species known to have antioxidant, antimicrobial and aromatic
properties and the existence of Salvia species is known to date back to 753 BC. This species,
whose medicinal properties were first discovered by the Romans at this time, is used in a wide
variety of fields. Salvia species, which have a content especially rich in essential oils, are often
used in cosmetic, medical, and industrial fields. In addition, it has been shown by past studies
that the root parts of salvia species contain terpenoids, polyphenols and essential fatty acids.
Numerous cancer studies have been conducted with salvia species, and it seems that not enough
research has been done for salvia cadmica. In this study, IC50 values for Total Antioxidant
Status (TAS) were determined by ethanol extract taken from the above-ground parts of Salvia
cadmica. Then, using the MTT method, their cytotoxicity in the human colorectal
adenocarcinoma cancer cell line (HT-29) and healthy fibroblast cell line (L929) was
determined. Accordingly, Salvia Cadmica's IC50 dose was 64.22 ± 6.31 for the HT-29 cell line.
For healthy fibroblast cell lines, this ratio was higher than 400 μg/mL.
0007-01-01T00:00:00Z