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dc.contributor.authorTutar, Yusuf
dc.contributor.authorHarman, James G.
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T10:17:49Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T10:17:49Z
dc.date.issued2006
dc.identifier.issn0003-9861
dc.identifier.urihttps://dx.doi.org/10.1016/j.abb.2006.06.019
dc.identifier.urihttps://hdl.handle.net/20.500.12418/10772
dc.descriptionWOS: 000240715000009en_US
dc.descriptionPubMed ID: 16934214en_US
dc.description.abstractExpression of catabolite-sensitive operons in Escherichia coli is cAMP-dependent and mediated through the CRP:cAMP complex binding to specific sequences in DNA. Five specific ionic or polar interactions occur in cAMP binding pocket of CRP. E72 interacts with the cAMP 2' OH, R82 and S83 interact with the negatively charged phosphate moiety, and T127 and S128 interact with the adenine ring. There is evidence to suggest that E72 and R82 may mediate an essential CRP molecular switch mechanism, Therefore, stimulation of CRP transcription activation was examined by perturbing these residues. Further, CRP:cAMP complex was treated with a specific DNA sequence containing the lac CRP binding site along with RNA polymerase to mimic in vivo conditions. Biochemical and biophysical results revealed that regulation of transcription activation depends on alignment of CRP tertiary structure through inter-domain communication and it was concluded that positions 72 and 82 are essential in the activation of CRP by cAMP. (c) 2006 Elsevier Inc. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.isversionof10.1016/j.abb.2006.06.019en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecttranscription activationen_US
dc.subjectDNA footprintingen_US
dc.subjectoperonen_US
dc.subjectfilter bindingen_US
dc.subjectCDen_US
dc.subjectFTIRen_US
dc.titleEffect of salt bridge on transcription activation of CRP-dependent lactose operon in Escherichia colien_US
dc.typearticleen_US
dc.relation.journalARCHIVES OF BIOCHEMISTRY AND BIOPHYSICSen_US
dc.contributor.departmentTexas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA -- Cumhuriyet Univ, Dept Chem, TR-58140 Sivas, Turkey -- S Plains Coll, Dept Chem, Levelland, TX 79336 USAen_US
dc.identifier.volume453en_US
dc.identifier.issue2en_US
dc.identifier.endpage223en_US
dc.identifier.startpage217en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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