dc.contributor.author | Gulec, Haci Ali | |
dc.contributor.author | Gurdas, Sevim | |
dc.contributor.author | Albayrak, Nedim | |
dc.contributor.author | Mutlu, Mehmet | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:07:00Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:07:00Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 1226-8372 | |
dc.identifier.uri | https://dx.doi.org/10.1007/s12257-010-0046-7 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9749 | |
dc.description | WOS: 000285862900016 | en_US |
dc.description.abstract | The aim of this study was to produce galacto-oligosaccharides (GOS) from lactose using beta-galactosidase from Aspergillus oryzae immobilized on a low-pressure plasma-modified cellulose acetate (CA) membrane Specifically, a novel method was developed for multilayer enzyme immobilization involving polyethyleneimine (PEI)-enzyme aggregate formation and growth on a CA membrane A large amount of enzyme (997 mu g/cm(2) membrane) was immobilized with 66% efficiency The K(m) value for the immobilized enzyme was estimated to be 48 mM, which indicates decreased affinity for the substrate, whereas the Vmax value was smaller The immobilized enzyme showed good storage and operational stability The half-life of the immobilized enzyme on the membrane was about 1 month at 30 degrees C and similar to 60 h at 60 degrees C Maximum GOS production of 27% (w/w) was achieved with 70% lactose conversion from 320 g/L of lactose at pH 4 5 and 60 degrees C Trisaccharides were the major types of GOS formed and accounted for about 75% of the total GOS produced Based on these results, immobilized enzyme technology could be applied to GOS production from lactose | en_US |
dc.description.sponsorship | Yuzuncu Yil University [2008-FBE-D011]; Scientific and Technological Research Council of Turkey [1060116] | en_US |
dc.description.sponsorship | This work was supported in part by the Scientific Research Fund of Yuzuncu Yil University (No 2008-FBE-D011) and The Scientific and Technological Research Council of Turkey (Grant No 1060116) | en_US |
dc.language.iso | eng | en_US |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | en_US |
dc.relation.isversionof | 10.1007/s12257-010-0046-7 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | enzyme immobilization | en_US |
dc.subject | polyethyleneimine | en_US |
dc.subject | low-pressure plasma polymerization | en_US |
dc.subject | cellulose acetate membrane | en_US |
dc.subject | galactooligosaccharide | en_US |
dc.title | Immobilization of Aspergillus oryzae beta-Galactosidase on Low-pressure Plasma-modified Cellulose Acetate Membrane Using Polyethyleneimine for Production of Galactooligosaccharide | en_US |
dc.type | article | en_US |
dc.relation.journal | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | en_US |
dc.contributor.department | [Gulec, Haci Ali] Trakya Univ, Fac Engn & Architecture, Dept Food Engn, TR-22180 Edirne, Turkey -- [Gulec, Haci Ali -- Gurdas, Sevim -- Mutlu, Mehmet] Hacettepe Univ, Plasma Aided Bioengn & Biotechnol Res Grp, TR-06800 Ankara, Turkey -- [Gurdas, Sevim] Cumhuriyet Univ, Fac Engn, Dept Food Engn, TR-58140 Sivas, Turkey -- [Albayrak, Nedim] Hitit Univ, Fac Engn, Dept Food Engn, TR-19030 Corum, Turkey | en_US |
dc.contributor.authorID | Mutlu, Mehmet -- 0000-0001-7146-1937; MUTLU, MEHMET -- 0000-0001-9610-9817 | en_US |
dc.identifier.volume | 15 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.endpage | 1015 | en_US |
dc.identifier.startpage | 1006 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |