dc.contributor.author | Eroglu, C | |
dc.contributor.author | Yildiz, E | |
dc.contributor.author | Ozturk, M | |
dc.contributor.author | Pinarbasi, E | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:25:29Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:25:29Z | |
dc.date.issued | 2000 | |
dc.identifier.issn | 0001-723X | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/11704 | |
dc.description | WOS: 000086884600005 | en_US |
dc.description | PubMed ID: 10989689 | en_US |
dc.description.abstract | In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned sequenced expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (ETA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SLOVAK ACADEMIC PRESS LTD | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | hepatitis C virus | en_US |
dc.subject | core protein gene | en_US |
dc.subject | cloning | en_US |
dc.subject | nucleotide sequencing | en_US |
dc.subject | expression | en_US |
dc.subject | E. coli | en_US |
dc.subject | purification | en_US |
dc.subject | ELISA | en_US |
dc.subject | RT-PCR | en_US |
dc.title | A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus | en_US |
dc.type | article | en_US |
dc.relation.journal | ACTA VIROLOGICA | en_US |
dc.contributor.department | Bilkent Univ, Fac Sci, Dept Mol Biol & Genet, Ankara, Turkey -- Cumhuriyet Univ, Fac Med, Dept Med Biol & Genet, TR-58140 Sivas, Turkey | en_US |
dc.contributor.authorID | Erdal, Esra -- 0000-0001-7264-0574; Eroglu, Cagla -- 0000-0002-7204-0218; | en_US |
dc.identifier.volume | 44 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.endpage | 33 | en_US |
dc.identifier.startpage | 29 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |