ADSORPTION OF BOVINE SERUM-ALBUMIN ONTO ACRYLAMIDE-MALEIC ACID HYDROGELS

Abstract

In this study, acrylamide-maleic acid hydrogels containing different amounts of maleic acid have been prepared by irradiating with gamma radiation. They have been used in experiments on bovine serum albumin (BSA) adsorption. Acrylamide-maleic acid hydrogel containing 60 mg of maleic acid and irradiated at 5.20 kGy has been used for swelling and diffusion studies in water, solutions of NaCl and BSA. For this hydrogel, maximum and minimum swellings have been observed with water (1680%) and the solution of BSA (1085%), respectively. Diffusions of water, NaCl and BSA within hydrogels have been found as non-Fickian types of diffusion. In the experiments of BSA adsorption, Langmuir type adsorption has been found. The hydrogel prepared with 60 mg maleic acid and having been irradiated at 2.00 kGy has been found to be the best adsorption system for BSA. The adsorption capacity of acrylamide hydrogel has been increased by the addition of maleic acid almost 1.5-3-fold.