Novel natural spider silk embedded electrospun nanofiber mats for wound healing
Künye
Kerim Emre Öksüz, Nese Kurt Özkaya, Zeynep Deniz Şahin İnan, Ali Özer, Novel natural spider silk embedded electrospun nanofiber mats for wound healing, Materials Today Communications, Volume 26, 2021, 101942, ISSN 2352-4928,Özet
In recent years, electrospun nanofiber mats based on biopolymers have been extensively investigated for tissue
and biomedical engineering, mainly because of remarkable morphological similarity with the natural extracellular
matrix. The current study focuses on the preparation of novel natural spider silk (SS) embedded Poly(vinyl
alcohol)(PVA)/Sodium alginate (NaAlg) wound dressings with desirable properties for a wound dressing
application. The nanofibers’ surface morphology and structure were observed by a field emission scanning
electron microscope (FE-SEM). In-vivo evaluation of PVA/NaAlg based electrospun nanofiber mats as a wound
dressing material and their comparison to commercially available wound dressings was carried out by in-vivo
tests on rabbit models. Some morphometric parameters such as counting cells, blood vessels, endothelial cells,
determination of the area of the wound closure, wound healing performance, speed of wound healing and
collagen thickness were investigated using OM and FE-SEM post-processing. Furthermore, in-vitro biocompatibility
and cellular behavior such as adhesion and proliferation of mouse fibroblast cells (L929) were studied by
XTT assay on developed nanofiber mats. The results of this experimental study indicated that the natural spider
silk embedded electrospun nanofiber mat (PVA/NaAlg/SS) accelerated the rate of wound healing compared to
other groups by improving the collagen formation rate, proliferative cell activity as well as decreasing the inflammatory
cell amount. Furthermore, the results of the in-vivo and in-vitro experiments suggest that novel PVA/
NaAlg/SS nanofiber mats might be a fascinating bioactive wound dressing for clinical applications.
Kaynak
Materials Today CommunicationsCilt
26Sayı
101942Bağlantı
https://www.sciencedirect.com/science/article/abs/pii/S2352492820329536?via%3Dihubhttps://hdl.handle.net/20.500.12418/13019