Expression levels of BAP1, OGT, and YY1 genes in patients with eyelid tumors
Citation
Ayca Tas, Department of Nutrition and Diet, Faculty of Health Sciences, Sivas Cumhuriyet University, 58140 Sivas, Turkey, Phone: +90 3462580000, E-mail: aycatas@cumhuriyet.edu.tr. https://orcid.org/0000-0002-7132-1325 Erkan Gumus, Department of Histology and Embryology, Faculty of Medicine, Adnan Menderes University, Aydın, Turkey, E-mail: erkangumus@yahoo.com. https://orcid.org/0000-0001-6432-7457 Esma Ozmen, Department of Medical Biochemistry, Faculty of Medicine, Nigde Omer Halis Demir University, Nigde, Turkey, E-mail: ozmenesma07@gmail.com. https://orcid.org/0000-0003-3223-6854 Haydar Erdogan, Department of Ophthalmology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey, E-mail: herdogan@cumhuriyet.edu.tr. https://orcid.org/0000-0002-9337-6968 Yavuz Silig, Department of Medical Biochemistry, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey, E-mail: ysilig@cumhuriyet.edu.tr. https://orcid.org/0000-0002-0562-7457Abstract
Objectives: The aim of this study was to investigate BAP1,
OGT and YY1 genes and protein levels in 12 samples
(8 males, 4 females) of eyelid tumor tissue with basal cell
carcinoma (BCC) and 12 normal control subjects (8 males, 4
females).
Methods: The expression levels of these genes were
determined with RT-PCR and the protein levels and
expression using ELISA and IHC methods, respectively.
Results: In RT-PCR analysis, statistically significant
upregulated expression was determined of 1.84-fold of
BAP1, 2.85-fold of OGT and 3.06-fold of YY1 genes (p < 0.05).
In the patient group, compared to the control group, there
was a similar statistically significant strong correlation
between the proteins (BAP1 and YY1; r = 0.850, BAP1 and
OGT; r = 0.811, OGT and YY1; r = 0.755) (p < 0.05). In the
ELISA and IHC analysis methods, a significant increase in
BAP1 and YY1 protein expression levels was observed
compared to the control group (p < 0.05).
Conclusions: The study results demonstrated that BAP1
and YY1 genes and protein levels were upregulated in
eyelid tumor tissue with BCC.