Circulating serum miR-200c and miR-34a-5p as diagnostic biomarkers for endometriosis
Date
2021Metadata
Show full item recordAbstract
Objective: Endometriosis is defined by the presence of endometrial glands and stroma grow in areas
outside the uterus. A simple blood test for endometriosis-specific biomarkers would offer a more timely
accurate diagnosis of the disease and could lead to earlier treatment intervention. Alterations in
microRNA (miRNA) levels in blood may reflect changes during normal physiologic processes and have
been related to several pathologic conditions, including gynecologic diseases. In the present study, we
aim to evaluate the level of serum miR-34a-5p and miR-200c from women with and without
endometriosis, and to explore the potential of miRNAs as reliable non-invasive biomarkers in the
diagnosis of endometriosis.
Methods: Expression levels of miRNAs were performed by quantitative real-time polymerase chain
reaction (qRT-PCR). Serum cancer antigen 125 (CA-125) levels were analyzed by autoanalyzer.
Results: miR-34a-5p expression levels were decreased and miR-200c expression levels were increased in
the endometriosis patients compared to the control group. According to the areas under the ROC curve
(AUC) values, miR-200c and miR-34a-5p may serve as biomarkers for the diagnosis of endometriosis.
Serum miR-34a-5p and miR-200c had a sensitivity of 78.95 % and 100 % and a specificity of 49.12 % and
100 %, respectively, for the detection of endometriosis.
Conclusion: Serum miRNAs may provide a promising opportunity for diagnosis of endometriosis.
Understanding the role of circulating miRNAs will serve a better comprehension of the systemic effects of
endometriosis and offer options for new treatments. It is clear that more work is needed in this area.