Nanocomposite smart hydrogel based on sepiolite nanochannels/N-isopropyl acrylamide/itaconic acid/acrylamide for invertase immobilization
Özet
Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as
a support for invertase. Spectrophotometric and thermal analysis methods were used for the
characterization. Lower critical solution temperature values of SH and NSH were found as 32.68°C
and 30.44°C, respectively. From the pH-responsive swellings performed at three different tempera tures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was
immobilized onto SH and NSH (SH−I and NSH−I). The optimum pH and temperature values were
found. Michaelis constant and maximum reaction rate were calculated. NSH−I showed excellent
thermal, operational and pH stability