Buffaloes as new hosts for Anaplasma capra: Molecular prevalence and phylogeny based on gtlA, groEL, and 16S rRNA genes
Citation
Sahin, O. F., Erol, U., & Altay, K*. (2022). Buffaloes as new hosts for Anaplasma capra: Molecular prevalence and phylogeny based on gtlA, groEL, and 16S rRNA genes. Research in Veterinary Science, 152, 458-464. https://doi.org/10.1016/j.rvsc.2022.09.008Abstract
Anaplasma capra is a tick-borne pathogen that was discovered for the first time in goats in China in 2012. The
studies carried out from the first detection in China to the present have revealed the presence of this species in
eight countries including Angola, France, Iranian, South Korea, Kyrgyzstan, Malaysia, Spain, and Türkiye in three
continents (Africa, Asia, and Europe). It has also been determined that humans, sheep, cattle, dog, and wild
animals are the hosts of A. capra. It was investigated whether water buffaloes were the host of A. capra using
nested-PCR and DNA sequencing in this study. The prevalence of A. capra in Turkish water buffalo herds was
investigated and phylogenetic analyzes were performed on the basis of gltA, groEL, and 16S rRNA genes. A total
of 364 water buffalo blood samples were examined in terms of A. capra using gltA gene species-specific nested-
PCR. A. capra were detected in 52 of 364 (14.28%) blood samples. There was no statistically significant dif-
ference between the prevalence, gender, and age parameters. The gltA, groEL, and 16S rRNA genes in randomly
selected three positive samples were sequenced. A. capra isolates obtained from water buffaloes in this study
shared 85.20–100%(gltA), 89.84–100%(groEL), and 99.82–100%(16S rRNA) nucleotide similarity with A.capra
isolates present in GeneBank. Phylogenetic analyses of gtlA and groEL genes revealed that A. capra divided in two
different genogroups.
In conclusion, this study revealed that water buffalo is a new host of A. capra. However, comprehensive studies
are needed to determine the pathogenicity, vectors, and biological properties of A. capra in this new host.
Source
Research in Veterinary ScienceVolume
152URI
https://reader.elsevier.com/reader/sd/pii/S0034528822002892?token=8580AAC90D81FF7B2F2F98B82DE27ADE21574BA9A8B7D04041250FFC0C1338374CA80BE071E1BE736BAC1822BF748382&originRegion=eu-west-1&originCreation=20230403100630https://hdl.handle.net/20.500.12418/13258