Death receptor-dependent apoptosis and cell cycle delay induced by bioymifi in human cervical cancer cells
Abstract
Abstract: In chemotherapy applied against cervical cancer, non-specific cytotoxicity and drug resistance that develops
over time are trying to be overcome. Therefore, the development of effective and innovative chemotherapeutic drugs for
the treatment is among the priority issues in the medical field. The anticancer activity of the Bioymifi, which can activate
apoptosis by inducing DR-5 clustering and aggregation against the human cervical cancer cell line, was investigated in
the current study. The cytotoxic activity of Bioymifi on the HeLa cell line was identified using XTT assay. The pathway
of the cell death mechanism was analyzed through the cell cycle and Annexin V assays by the flow cytometry. DAPI
staining assay was applied under fluorescence microscopy to examine the nuclear morphology. Bioymifi appeared to
have a remarkable IC50 value (11.75μM) against HeLa cells. The cell cycle analysis demonstrated the increase of
Bioymifi cured HeLa cells in the S phase. And also, 11.75µM of Bioymifi caused a significantly higher apoptotic effect
compared to control. In addition, in vitro immunofluorescence experiments of this study represented that Bioymifi
reduced Ki-67 localization in HeLa cells. Bioymifi has significantly anticancer actions in Human cervix cancer in vitro
and can be combined with standard treatment.