IN VITRO INVESTIGATION OF THE INHIBITORY EFFECT OF HEPATOTOXIC DIETHYLNITROSAMINE COMPOUND ON XANTHINE OXIDASE ENZYME
Date
aralık 202Metadata
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Aim: It was aimed to examine the inhibition effect of diethyl nitrosamine compound, one of the
nitrosole compounds known to have carcinogenic and hepato-toxic effects, on xanthine oxidase enzyme
in vitro.
Materials-Method: For this purpose, the inhibition effect of the diethyl nitrosamine compound
prepared at 3 different concentrations (2mM, 4mM, 6mM) on the xanthine oxidase enzyme in the
substrate medium at 4 different concentrations (0.3 mg/mL, 0.5 mg/mL, 1 mg/mL, 2 mg/mL) was
examined. Xanthine oxidase enzyme activity was determined spectrophotometrically by reading the
absorbance of urate formed from hypoxanthine at a wavelength of 290 nm according to the Worthington.
Michaelis Menten and Lineweaver Burk graphs were drawn with the data obtained. Km, Vmax values of
the enzyme were calculated with the help of the equation obtained from the drawn Lineweaver Burk
plot. Additionally, the IC50 values of the enzyme were calculated with the help of graphics. GraphPad
Prism 6 program was used for drawing graphs and calculations.
Results: It was observed that while the Vmax values obtained as a result of the calculations decreased,
the Km values remained constant. In the light of the Lineweaver Burk plot and calculations, it was
concluded that the diethyl nitrosamine compound has a non-competitive inhibition on the xanthine
oxidase enzyme.
Conclusion: We found that the diethyl nitrosamine compound non-competitively inhibits the
xanthine oxidase enzyme, which is known to play important roles in many biological processes. The
compound diethyl nitrosamine, which is known to have hepatotoxic and carcinogenic effects even
at low doses, binds reversibly to the structure of the enzyme, but is very harmful in terms of side
effects. It is not possible to use it for therapeutic purposes, and exposure to this compound may cause
worse pathophysiological processes by inhibiting many enzymes and pathways, including xanthine
oxidase