Evaluated periodontal tissues and oxidative stress in rats with neuropathic pain-like behavior
Tarih
Ekim 2023Üst veri
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Background Oxidative stress has a critical effect on both persistent pain states and periodontal disease. Voltage-gated sodium
NaV1.7 (SCN9A), and transient receptor potential ankyrin 1 (TRPA1) are pain genes. The goal of this study was to investi gate oxidative stress markers, periodontal status, SCN9A, and TRPA1 channel expression in periodontal tissues of rats with
paclitaxel-induced neuropathic pain-like behavior (NPLB).
Methods and results Totally 16 male Sprague Dawley rats were used: control (n=8) and paclitaxel-induced pain (PTX)
(n=8). The alveolar bone loss and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were analyzed histometrically and immu nohistochemically. Gingival superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities
(spectrophotometric assay) were measured. The relative TRPA1 and SCN9A genes expression levels were evaluated using
quantitative real-time PCR (qPCR) in the tissues of gingiva and brain. The PTX group had significantly higher alveolar bone
loss and 8-OHdG compared to the control. The PTX group had significantly lower gingival SOD, GPx and CAT activity
than the control groups. The PTX group had significantly higher relative gene expression of SCN9A (p=0.0002) and TRPA1
(p=0.0002) than the control in gingival tissues. Increased nociceptive susceptibility may affect the increase in oxidative
stress and periodontal destruction.
Conclusions Chronic pain conditions may increase TRPA1 and SCN9A gene expression in the periodontium. The data of the
current study may help develop novel approaches both to maintain periodontal health and alleviate pain in patients suffering
from orofacial pain.