Determination of trace cobalt (II) in spices samples by ultrasonic assisted cloud point extraction with spectrophotometry

Abstract

In this study, ultrasonic assisted cloud point extraction (UA-CPE) method was developed for the determination of Cobalt (II) (Co(II)) in spices. After extraction and preconcentration, the Co(II) contents of the samples were determined by UV-VIS. After complexing with Ponceau Xylidine (PX) and EDTA in the presence of cationic surfactant, CTAB at pH 4.0 with Britton-Robinson (BR) buffer, Co(II) ion was withdrawn the surfactant-rich phase of nonionic surfactant, Triton X-114 (TX-114). The concentrated surfactant-rich phase containing the analyte was diluted with ethanol to a volume of 1.0 mL and then analyzed by UV-VIS spectrophotometer. The various analytical parameters affecting the UA-CPE yield were investigated and optimized. Analytical data achieved after optimization: limits of detection (LOD) and: limits of quantification (LOQ) are 0.76 and 2.59 mu g L-1, respectively; The calibration curve is rectilinear for Co(II) with changed calibration sensitivity in the range of 1-10 and 10-210 mu g L-1. The precision (as RSD%) is 4.8. This optimized method was applied in the analysis of various spice samples. (c) 2023 Elsevier B.V. All rights reserved.