Use of Fe3O4@MPTMS-Dithizone Magnetic Nanoparticles as Solid Phase Sorbent for Sensitive Analysis of Sibutramine Molecules in Herbal Slimming Products
Date
June 07, 2Metadata
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Abstract: Background: A new enrichment and sensitive determination method, which includes HPLCDAD
analysis after Magnetic Solid Phase Extraction (MSPE), has been developed for trace analysis of
Sibutramine molecules in herbal slimming products. Sibutramine is one of the most adulterated drug molecules
in herbal products.
Method: In the proposed method, Sibutramine molecules were pre-concentrated by using
Fe3O4@MPTMS-Dithizone magnetic sorbent synthesized in our laboratory. Desorption of Sibutramine
molecules from the sorbent phase was carried out by using acetonitrile: methanol (1:1) solvent in the
presence of pH 8.0 buffer before chromatographic determinations.
Results: Analytical parameters of the method, such as linear range, enrichment factor, and determination
limit, were determined after optimizing experimental variables such as interaction time, desorption solvent,
pH, etc. The sibutramine molecule was analyzed by isocratic elution of acetonitrile and KH2PO4 (pH
3.0, 0.05 M) (40:60) with a DAD detector at 223 nm wavelength. Limit of detection (LOD) value was
calculated as 1.43 ng mL-1.
Conclusion: Relative standard deviations (RSD) were below 3.20% for determinations of model solutions,
including 100 ng mL-1 of Sibutramine. Finally, the developed method has been applied to herbal
slimming tea samples with quantitative recovery experiments.