dc.contributor.author | Özge Çevik | |
dc.contributor.author | Zelal Adıgüzel | |
dc.contributor.author | Ahmet Tarık Baykal | |
dc.contributor.author | Azize Şener | |
dc.date.accessioned | 23.07.201910:49:13 | |
dc.date.accessioned | 2019-07-23T16:37:25Z | |
dc.date.available | 23.07.201910:49:13 | |
dc.date.available | 2019-07-23T16:37:25Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1300-0152 | |
dc.identifier.uri | http://www.trdizin.gov.tr/publication/paper/detail/TWpRME1EUTJOZz09 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/3378 | |
dc.description.abstract | Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-α in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-α and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-α and/or 50 mM iNOS specific inhibitor, such as at 1400 W for 1 h at 37 °C in the presence of 5 µM ADP. According to the results, the levels of PS exposure and P-selectin binding were significantly higher in TNF-α + ADP platelets, which decreased with the addition of 1400 W. A significant induction in cGMP, Cyt-c, and Ca2+ levels was observed in platelets treated with TNF-α + ADP. On the other hand, the upregulation of the apoptotic gene caspase-3 and iNOS expression levels in TNF-α-treated and ADP-activated platelets was significantly reversed with the addition of 1400 W. These data demonstrate that TNF-α promotes iNOS expression through caspase-3 stimulation in human platelets, and its concomitance leads to the triggering of apoptosis-mediated inflammation upon platelet activation. | en_US |
dc.description.abstract | Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-α in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-α and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-α and/or 50 mM iNOS specific inhibitor, such as at 1400 W for 1 h at 37 °C in the presence of 5 µM ADP. According to the results, the levels of PS exposure and P-selectin binding were significantly higher in TNF-α + ADP platelets, which decreased with the addition of 1400 W. A significant induction in cGMP, Cyt-c, and Ca2+ levels was observed in platelets treated with TNF-α + ADP. On the other hand, the upregulation of the apoptotic gene caspase-3 and iNOS expression levels in TNF-α-treated and ADP-activated platelets was significantly reversed with the addition of 1400 W. These data demonstrate that TNF-α promotes iNOS expression through caspase-3 stimulation in human platelets, and its concomitance leads to the triggering of apoptosis-mediated inflammation upon platelet activation. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Biyoloji | en_US |
dc.title | Tumor necrosis factor-alpha induced caspase-3 activation-related iNOS gene expression in ADP-activated platelets | en_US |
dc.type | article | en_US |
dc.relation.journal | Turkish Journal of Biology | en_US |
dc.contributor.department | Sivas Cumhuriyet Üniversitesi | en_US |
dc.identifier.volume | 41 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.endpage | 40 | en_US |
dc.identifier.startpage | 31 | en_US |
dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US] |