Sıçanlarda Karaciğer İskemi Reperfüzyon Hasarında Piknogenol®'ün İyileştirici Etkileri
Tarih
2017Yazar
Mehmet TokaçMerve Bacanlı
Ersin Gürkan Dumlu
Sevtap Aydın
Merve Engin
Birkan Bozkurt
Abdüssamed Yalçın
Özcan Erel
Mehmet Kılıç
Nurşen Başaran
Üst veri
Tüm öğe kaydını gösterÖzet
Amaç: Piknogenol® (PYC®), Pinus maritima bitkisinin kabuğundan elde edilen ve birkaç tip fenolik içerikten oluşan, suda çözünür standardize bir Bulgular: TOS, TTL, MPO, AOPP, ALT, AST and LDH düzeyleri IR+PYC® grubunda IR grubuna göre anlamlı ölçüde düşük bulundu (p<0.05). TAS düzeyi IR+PYC® grubunda IR grubuna göre anlamlı ölçüde yüksek bulundu (p<0.05). DNA hasarı değerlendirildiğinde PYC® verilen grupta DNA iskemi grubuna göre anlamlı ölçüde azaldığı saptandı (p<0.05).Sonuç: Mevcut bulgular, piknogenol tedavisinin IR ile indüklenmiş oksidatif hasarı DNA hasarını azaltarak ve antioksidan durumu arttırarak da önlediğini göstermektedir. Pycnogenol® (PYC®), a standardized extract from the bark of Pinus maritima, consists of different phenolic compounds. PYC® has shown to have protective effects on chronic diseases such as diabetes, asthma, cancer, and immune disorders. The aim of this study was to determine the effects of PYC® against the DNA damage and biochemical changes in blood, liver, and lung tissues of ischemia-reperfusion (IR)-induced Wistar albino rats. Materials and Methods: A sham group, IR injury-induced group, and IR+PYC® group were formed. Ischemia was induced and sustained for 45 min, then the ischemic liver was reperfused, which was sustained for a further 120 min at the end of this period. After anesthesia and before the IR inducement, 100 mg/kg PYC® was given to the IR+PYC® group through intraperitoneal injections. The total oxidant (TOS) and total antioxidant status (TAS), total thiol levels (TTL), advanced oxidation protein products (AOPP), and biochemical parameters [myeloperoxidase (MPO), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH)] in the rats were analyzed using spectrophotometric methods and DNA damage was assessed using single-cell gel electrophoresis. Results: The levels of TOS, TTL, MPO, AOPP, ALT, AST, and LDH were significantly decreased in the IR+PYC® group compared with the IR group (p<0.05). The levels of TAS were significantly increased in the IR+PYC® group compared with the IR group (p<0.05). PYC® reduced the DNA damage when compared with the IR group (p<0.05). Conclusion: The present results suggest that PYC® treatment might have a role in the prevention of IR-induced oxidative damage by decreasing DNA damage and increasing antioxidant status.
Kaynak
Turkish Journal of Pharmaceutical SciencesCilt
14Sayı
3Bağlantı
http://www.trdizin.gov.tr/publication/paper/detail/TWpRME1qRTBOQT09https://hdl.handle.net/20.500.12418/3429
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- Makale Koleksiyonu [3404]
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