dc.contributor.author | Karakoyun, Berna | |
dc.contributor.author | Ertas, Busra | |
dc.contributor.author | Yuksel, Meral | |
dc.contributor.author | Akakin, Dilek | |
dc.contributor.author | Cevik, Ozge | |
dc.contributor.author | Sener, Goksel | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:38:07Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:38:07Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1440-1681 | |
dc.identifier.uri | https://dx.doi.org/10.1111/1440-1681.12894 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6267 | |
dc.description | WOS: 000433569300009 | en_US |
dc.description | PubMed ID: 29164668 | en_US |
dc.description.abstract | Riboflavin (RF) has been found to be a promising antioxidant and/or anti-inflammatory agent in several studies. However, the effect of RF against acetic acid (AA)-induced colonic injury is currently unknown. This study aimed to investigate the potential antioxidant and protective effects of RF in a rat model of ulcerative colitis. Starting immediately after the colitis induction (AA+RF group) or 1week before the colitis induction (RF+AA+RF group), the rats were treated with RF (25mg/kg per day; p.o.) for 3days. The control and AA groups received saline (1mL; p.o.) whereas AA+SS group (positive control) received sulfasalazine (100mg/kg per day; p.o.) for 3days. Colonic samples were taken for the biochemical and histological assessments on the third day. High damage scores, elevated tissue wet weight index (WI), tissue myeloperoxidase (MPO) activity, 8-hydroxy-2-deoxyguanosine levels and chemiluminescence values, and a pronounced decrease in antioxidant glutathione (GSH) levels of the AA group were all reversed by RF pretreatment (RF+AA+RF group) and SS treatment (AA+SS group) (P<.05-.001). Tissue WI, MPO activity and GSH levels were not statistically changed in the AA+RF group. Western blot analysis revealed that the decreased protein expressions of tissue collagen (COL) 1A1, COL3A1 and transforming growth factor-1 in the AA group were elevated in all the treatment groups (P<.05-.001). In conclusion, RF exerts both the antioxidant and anti-inflammatory effects against AA-induced colonic inflammation by suppressing neutrophil accumulation, inhibiting reactive oxidant generation, preserving endogenous glutathione, improving oxidative DNA damage and regulating inflammatory mediators, suggesting a future potential role in the treatment and prevention of ulcerative colitis. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | WILEY | en_US |
dc.relation.isversionof | 10.1111/1440-1681.12894 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | acetic acid | en_US |
dc.subject | colitis | en_US |
dc.subject | oxidative damage | en_US |
dc.subject | riboflavin | en_US |
dc.title | Ameliorative effects of riboflavin on acetic acid-induced colonic injury in rats | en_US |
dc.type | article | en_US |
dc.relation.journal | CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | en_US |
dc.contributor.department | [Karakoyun, Berna] Marmara Univ, Dept Basic Hlth Sci, Fac Hlth Sci, Istanbul, Turkey -- [Ertas, Busra -- Sener, Goksel] Marmara Univ, Dept Pharmacol, Fac Pharm, Istanbul, Turkey -- [Yuksel, Meral] Marmara Univ, Vocat Sch Hlth Related Profess, Dept Med Lab, Istanbul, Turkey -- [Akakin, Dilek] Marmara Univ, Sch Med, Dept Histol & Embryol, Istanbul, Turkey -- [Cevik, Ozge] Cumhuriyet Univ, Dept Biochem, Fac Pharm, Sivas, Turkey | en_US |
dc.identifier.volume | 45 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.endpage | 572 | en_US |
dc.identifier.startpage | 563 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |