dc.contributor.author | Kocyigit, Umit M. | |
dc.contributor.author | Dastan, Sevgi Durna | |
dc.contributor.author | Taslimi, Parham | |
dc.contributor.author | Dastan, Taner | |
dc.contributor.author | Gulcin, Ilhami | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:39:00Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:39:00Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1095-6670 | |
dc.identifier.issn | 1099-0461 | |
dc.identifier.uri | https://dx.doi.org/10.1002/jbt.22000 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6454 | |
dc.description | WOS: 000419943200002 | en_US |
dc.description | PubMed ID: 28960668 | en_US |
dc.description.abstract | In this study, carbonic anhydrase (CA) enzyme was purified and characterized from blood samples of Kangal Akkaraman sheep and inhibitory properties on certain antibiotics were examined. CA purification was composed of preparation of the hemolysate and conducting the Sepharose-4B-tyrosine-sulfanilamide affinity gel chromatography in having specific activity of 11626EUmg(-1), yield of 14.40%, and 242.76-fold purification. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed to assess the enzyme purity and a single band was observed. Some antibiotics were exhibited in vitro inhibition on the CA activity. IC50 values of these inhibitors were calculated by plotting activity percentage. IC50 values of certain drugs (dexamethasone; caffeine; metamizole sodium; tetramisol; ceftiofur HCl; ivermectin; tavilin 50; penokain G; neosym; and sulfamezathine) were found as 0.38, 8.24, 285.53, 114.77, 5.33, 2.76, 27.58, 213.50, 208.28, and 36.60M, respectively. K-i values of different drugs on Kangal Akkaraman sheep blood CA activity were found in the range of 0.21 +/- 0.038-266.64 +/- 37.11 mu M. | en_US |
dc.description.sponsorship | Cumhuriyet University Research Fund (CUBAP) [SHMYO-011] | en_US |
dc.description.sponsorship | We acknowledge with great pleasure the partly support provided by Cumhuriyet University Research Fund (CUBAP), project no: SHMYO-011. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | WILEY | en_US |
dc.relation.isversionof | 10.1002/jbt.22000 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | carbonic anhydrase | en_US |
dc.subject | drugs | en_US |
dc.subject | enzyme purification | en_US |
dc.subject | enzyme inhibition | en_US |
dc.subject | Kangal Akkaraman sheep | en_US |
dc.title | Inhibitory effects of some drugs on carbonic anhydrase enzyme purified from Kangal Akkaraman sheep in Sivas, Turkey | en_US |
dc.type | article | en_US |
dc.relation.journal | JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | en_US |
dc.contributor.department | [Kocyigit, Umit M.] Cumhuriyet Univ, Vocat Sch Hlth Serv, TR-58140 Sivas, Turkey -- [Dastan, Sevgi Durna] Cumhuriyet Univ, Div Biometry & Genet, Dept Anim Nutr & Zootech, Fac Vet Med, TR-58140 Sivas, Turkey -- [Taslimi, Parham -- Gulcin, Ilhami] Ataturk Univ, Dept Chem, Fac Sci, TR-25240 Erzurum, Turkey -- [Dastan, Taner] Bingol Univ, Fac Arts & Sci, Dept Chem, TR-12000 Bingol, Turkey | en_US |
dc.contributor.authorID | GULCIN, Ilhami -- 0000-0001-5993-1668 | en_US |
dc.identifier.volume | 32 | en_US |
dc.identifier.issue | 1 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |