dc.contributor.author | Uyar, Ahmet | |
dc.contributor.author | Yaman, Turan | |
dc.contributor.author | Dogan, Abdulahad | |
dc.contributor.author | Uslu, Sema | |
dc.contributor.author | Keles, Omer Faruk | |
dc.contributor.author | Yener, Zabit | |
dc.contributor.author | Celik, Ismail | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:39:26Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:39:26Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1018-4619 | |
dc.identifier.issn | 1610-2304 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/6527 | |
dc.description | WOS: 000430372900044 | en_US |
dc.description.abstract | In the study 32 Wistar albino rats were divided into four group as control (Group Control, n=8), methotrexate (Group MTX, n=8), MTX + UDS (Group MTX+UDS, n=8) and Urtica dioica seed extract (UDS) (Group UDS, n=8). After the trial, blood and post-necropsy testicular tissue samples were taken. Histopathological examinations showed that methotrexate had an adverse impact on spermatogenesis by damaging testicles; however, such damages were substantially decreased in the Group MTX+UDS. In the immunohistochemical examinations glutathione peroxidase 1 (GPx1) immunoreactive areas was higher in the Group MTX + UDS compared to the Group MTX. Biochemical examinations revealed that the level of malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST) and catalase (CAT) enzymes levels statistically significantly differenced (p<0.001) in the Group MTX compared to the control, UDS and MTX+UDS groups. There were significant (p<0.05) differences the Group MTX from Group MTX+UDS. such as density, motility, dead-live sperm rate and abnormal sperm rate. Our study results showed that UDS prevented the damage occurred in the testicles according to histopathological, immunohistochemical, biochemical and spermatological findings. | en_US |
dc.description.sponsorship | Yuzuncu Yil University, Department of Scientific Research Projects [2015-HIZ-VF046, 1523] | en_US |
dc.description.sponsorship | We would like to thank to Yuzuncu Yil University, Department of Scientific Research Projects [Project Code: 2015-HIZ-VF046 (ID Number: 1523)]. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | PARLAR SCIENTIFIC PUBLICATIONS (P S P) | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Methotrexate | en_US |
dc.subject | Urtica dioica seed extract | en_US |
dc.subject | Pathology | en_US |
dc.subject | Testicles | en_US |
dc.title | IS METHOTREXATE-INDUCED TESTICULAR DAMAGE PREVENTABLE USING NETTLE SEED EXTRACT: A HISTOPATHOLOGICAL, IMMUNOHISTOCHEMICAL, BIOCHEMICAL AND SPERMATOLOGICAL EXAMINATION | en_US |
dc.type | article | en_US |
dc.relation.journal | FRESENIUS ENVIRONMENTAL BULLETIN | en_US |
dc.contributor.department | [Uyar, Ahmet] Mustafa Kemal Univ, Fac Vet Med, Dept Pathol, Antakya, Turkey -- [Yaman, Turan -- Keles, Omer Faruk -- Yener, Zabit] Yuzuncu Yil Univ, Fac Vet Med, Dept Pathol, Van, Turkey -- [Dogan, Abdulahad] Yuzuncu Yil Univ, Fac Pharm, Dept Biochim, Van, Turkey -- [Uslu, Sema] Cumhuriyet Univ, Fac Vet Med, Dept Histol & Embryol, Sivas, Turkey -- [Celik, Ismail] Yuzuncu Yil Univ, Fac Biosci, Dept Mol Biol & Genet, Van, Turkey | en_US |
dc.identifier.volume | 27 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.endpage | 2331 | en_US |
dc.identifier.startpage | 2320 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |