dc.contributor.author | Erkus, Oyluin | |
dc.contributor.author | Okuklu, Burcu | |
dc.contributor.author | Yenidunya, Ali F. | |
dc.contributor.author | Harsa, Sebnem | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T09:56:55Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T09:56:55Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0023-6438 | |
dc.identifier.issn | 1096-1127 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.lwt.2013.03.007 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/8172 | |
dc.description | WOS: 000337768400008 | en_US |
dc.description.abstract | Streptococcus thermophilus is a commonly used starter bacterium in dairy industry. It reduces the pH of milk rapidly and equilibrates the medium for the growth of Lactobacillus delbrueckii subsp. bulgaricus during yoghurt fermentation. Efforts to increase the diversity of artisanal yoghurt starters are not only important to bring new strains with novel and desirable characteristics, but also for the preservation of natural diversity which diminishes with the overuse and spread of industrial starters to natural resources. In the present study, 14 artisanal yoghurt samples were processed for the isolation of promising strains for yoghurt starter culture production and 66 strains were subsequently characterized. They were all identified as S. thermophilus using species-specific PCR and 16S rRNA gene sequencing. Genotypic diversity at the strain level was investigated by pulsed field gel electrophoresis (PFGE), and 22 homology groups were obtained. Further phenotypic characterization unearthed a significant phenotypic heterogeneity within homology groups, mostly with atypical novel character. Only 7 out of 66 strains showed S. thermophilus type-strain like phenotypic traits. Majority of the isolates were determined to be protease positive and fast milk acidifier to be used as yoghurt starter culture. (C) 2013 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | Government Planning Institution under the project "The Production of Cheese and Yoghurt Starter Cultures and Lactase Enzyme for the Dairy Industry: Traditional and Modern Solutions against Lactose Intolerance" | en_US |
dc.description.sponsorship | We would like to acknowledge Mert Sudagidan for his scientific assistance during the PFGE study, Elif Sinem Celik for her contribution to the bacterial isolation and further flavor profiling of co-culture growths in her thesis study, Elcin Soydemir for the phage sensitivity tests of the isolates in her thesis study, and Hatice Yavuzdurmaz for her contribution to the bacterial isolation. This study was supported by the Government Planning Institution under the project "The Production of Cheese and Yoghurt Starter Cultures and Lactase Enzyme for the Dairy Industry: Traditional and Modern Solutions against Lactose Intolerance". Prof. Dr. Nihat AKIN is acknowledged for kindly providing yogurt samples that was used for the isolation of microbial species. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.lwt.2013.03.007 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Streptococcus thermophilus | en_US |
dc.subject | Yoghurt | en_US |
dc.subject | Fingerprinting | en_US |
dc.subject | PFGE | en_US |
dc.subject | Starter | en_US |
dc.title | High genetic and phenotypic variability of Streptococcus thermophilus strains isolated from artisanal Yuruk yoghurts | en_US |
dc.type | article | en_US |
dc.relation.journal | LWT-FOOD SCIENCE AND TECHNOLOGY | en_US |
dc.contributor.department | [Erkus, Oyluin -- Okuklu, Burcu -- Harsa, Sebnem] IYTE, Izmir Inst Technol, Dept Food Engn, TR-35430 Izmir, Turkey -- [Yenidunya, Ali F.] Cumhuriyet Univ, Dept Med Biol, Sivas, Turkey | en_US |
dc.contributor.authorID | Harsa, Hayriye Sebnem -- 0000-0001-6794-299X; Yenidunya, Ali Fazil -- 0000-0002-9886-977X | en_US |
dc.identifier.volume | 58 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.endpage | 354 | en_US |
dc.identifier.startpage | 348 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |