Separation of lysozyme with magnetically stabilized spherical hydroxyapatite microcomposites in a continuous flow system

Abstract

The aim of this study was to produce a spherical and magnetic hydroxyapatite (HA) microcomposite to use as an adsorbent for the separation of lysozyme in a magnetically stabilized fluidized bed and to separate lysozyme from a protein mixture composed of human immunoglobulin G, human serum albumin, and lysozyme. For this purpose, spherical and magnetic HA microcomposites (50-100 m) were synthesized by suspension polymerization and characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, electron spin resonance, vibrating-sample magnetometry, Brunauer-Emmett-Teller analysis, and a swelling test. The specific surface areas of the pure HA and magnetic HA microcomposite were determined to be 72.25 and 151.53 m(2)/g, respectively. The swelling ratio of the spherical HA microcomposites was 150%. Adsorption experiments were conducted under conditions that differed in terms of pH, temperature, ionic strength, flow velocity, and magnetic field. According to the findings of the adsorption kinetic studies, the adsorption process was appropriate to pseudo-first-order kinetics. The separation of the lysozyme from the protein mixture was achieved by means of a 50 mM pH 7.0 phosphate buffer. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 1602-1610, 2013