The Role of NF-kappa B1A Promoter Polymorphisms on Coronary Artery Disease Risk

Abstract

Coronary artery disease (CAD), which is now regarded as a chronic inflammatory disease, is the leading cause of death worldwide. Nuclear factor (NF)-B is a transcription factor that plays an important role in the regulation of the immune system. NF-BIA is the inhibitory version of NF-B. This study is the first investigation of the association between CAD and NF-BIA-297 C/T, -826 C/T, -881 A/G polymorphisms in a Turkish population using PCR-RFLP method. The study population comprised 201 cases with CAD and 201 healthy controls. There was no significant difference in NF-B1A-297 C/T and -881 A/G in allele and genotype frequencies between case and control populations. The genotype frequency of NF-BIA-826TT in the patients with CAD was significantly higher than that of the controls (p=0.015, adjusted OR=7.09, 95% CI=1.95-25.70). The patients with CAD also had significantly higher carriage rate of NF-BIA-826T allele than the controls (p=0.03, OR=1.43, 95% CI=1.03-1.99). Linkage analysis indicated a close linkage among these three variants of NF-BIA (for case, (2)=85.35 and p<0.001; for control, (2)=21.58 p<0.001) and TTG, TTA and TCG haplotypes were associated with CAD (adjusted OR=2.54, 95% CI=0.88-7.27; p=0.001, adjusted OR=1.61, 95% CI: 0.64-4.02; p=0.04, adjusted OR=0.08, 95% CI=0.01-0.64; p<0.001, respectively). NF-BIA-826TT genotype may be a significant risk factor and a valuable marker for the development of CAD.