dc.contributor.author | Akkaya, Birnur | |
dc.contributor.author | Yenidunya, Ali Fazil | |
dc.contributor.author | Akkaya, Recep | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:03:46Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:03:46Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0141-8130 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.ijbiomac.2012.02.011 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9144 | |
dc.description | WOS: 000304230000016 | en_US |
dc.description | PubMed ID: 22387519 | en_US |
dc.description.abstract | A Thermoalkalophilic amylase was produced from an environmental bacterial isolate. The enzyme was then immobilized through its amino groups onto the epoxy rings of magnetic poly glycidyl methacrylate [m-poly (GMA)] beads. The free enzyme was active within a large pH range, between 7 and 12 and displayed the optimum activity at 95 degrees C and pH 10. The immobilization appeared to increase the stability of the enzyme as its bound form showed optimum activity at 105 degrees C and pH 11.0. Kinetic studies demonstrated that immobilized enzyme had higher K-m and lower V-max values. The activity of the free and bound enzyme was determined, at 37 degrees C and pH 10.0 and pH 11.0, respectively, in the presence of various organic solvents and detergents (5%, v/v). Results obtained indicated that detergents, sodium dodecyl sulfate (SDS) and TritonX-100, caused six fold increase and that various organic solvents also increased the activity of the amylase. (C) 2012 Elsevier B.V. All rights reserved. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.relation.isversionof | 10.1016/j.ijbiomac.2012.02.011 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Enzyme immobilization | en_US |
dc.subject | Poly glycidyl methacrylate | en_US |
dc.subject | Organic solvent tolerant | en_US |
dc.subject | Thermo-alkalophilic amylase | en_US |
dc.title | Production and immobilization of a novel thermoalkalophilic extracellular amylase from bacilli isolate | en_US |
dc.type | article | en_US |
dc.relation.journal | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | en_US |
dc.contributor.department | [Akkaya, Birnur -- Yenidunya, Ali Fazil] Cumhuriyet Univ, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey -- [Akkaya, Recep] Cumhuriyet Univ, Vocat Sch Hlth Serv, TR-58140 Sivas, Turkey | en_US |
dc.contributor.authorID | Yenidunya, Ali Fazil -- 0000-0002-9886-977X; AKKAYA, Recep -- 0000-0002-3477-7198 | en_US |
dc.identifier.volume | 50 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.endpage | 995 | en_US |
dc.identifier.startpage | 991 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |