dc.contributor.author | Arslan, Serdal | |
dc.contributor.author | Acikkol, Aysen | |
dc.contributor.author | Korkmaz, Ertan Mahir | |
dc.date.accessioned | 2019-07-27T12:10:23Z | |
dc.date.accessioned | 2019-07-28T10:06:46Z | |
dc.date.available | 2019-07-27T12:10:23Z | |
dc.date.available | 2019-07-28T10:06:46Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1300-0152 | |
dc.identifier.issn | 1303-6092 | |
dc.identifier.uri | https://dx.doi.org/10.3906/biy-1007-35 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/9717 | |
dc.description | WOS: 000298197800001 | en_US |
dc.description.abstract | Oylum Hoyuk falls within the borders of the province of Kills, located in the southern part of Turkey. It is an important Bronze Age settlement dating back to 5500 BC. In this study, the sex of 36 individuals found in Oylum Hoyuk was examined using morphological and genetic methods. In the morphological method, sex was identified in 14 female and 13 male individuals. The remaining 8 individuals could not be identified due to the poor preservation of their skeletal remains. Genetic sex was determined with the amplification of sex-specific X, Y centromeric alphoid repeats and ZFX-ZYF gene regions of DNA, mostly extracted from teeth. Based on ZFX-ZYF gene regions, sex was successfully determined using the PCR-RFLP method. The amplification results of ZFX-ZYF gene regions were also tested by applying the PCR-RFLP method. The amplification of X, Y centromeric alphoid repeats was successful in 29 individuals, while sex determination based on ZFX-ZFY fragments was carried out in 22 individuals. The vast majority of the results determined by analysis of ancient DNA were consistent with the morphological method. However, these 2 methods produced slightly different results for 5 individuals. Finally, the results indicated that ancient DNA analysis is a reliable method for sex determination, especially in poorly preserved and fragmented skeletal remains. | en_US |
dc.description.sponsorship | Research Council of Cumhuriyet University (CUBAP), Sivas, Turkey [F-251] | en_US |
dc.description.sponsorship | We would like to thank Professor Engin Ozgen, the head of the Oylum Hoyuk excavation, and Associate Professor Atilla Engin for the permit to study these skeletons. We would also like to thank Sibel Kizildag and Hasret Ozturk for their great contributions to our laboratory studies. This study has been supported by the Research Council of Cumhuriyet University (CUBAP, Project No: F-251), Sivas, Turkey. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY | en_US |
dc.relation.isversionof | 10.3906/biy-1007-35 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Ancient DNA | en_US |
dc.subject | sex determination | en_US |
dc.subject | Bronze Age | en_US |
dc.subject | Oylum Hoyuk | en_US |
dc.title | The optimization of aDNA extraction protocol and sex determination of Bronze Age individuals from Oylum Hoyuk (Kilis, Turkey) | en_US |
dc.type | article | en_US |
dc.relation.journal | TURKISH JOURNAL OF BIOLOGY | en_US |
dc.contributor.department | [Arslan, Serdal -- Korkmaz, Ertan Mahir] Cumhuriyet Univ, Fac Sci, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey -- [Acikkol, Aysen] Cumhuriyet Univ, Fac Literature, Dept Anthropol, TR-58140 Sivas, Turkey | en_US |
dc.identifier.volume | 35 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.endpage | 653 | en_US |
dc.identifier.startpage | 647 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |