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Öğe 5-Azo deoksisitidin’in ( Baz analogu) insan 11,16 ve X kromozomları üzerindeki etkisinin C ve bromodeoksi uridin (BrdU) bant teknikleriyle araştırılması(Cumhuriyet Üniversitesi, 1992) Özdemir, Öztürk; Sezgin, İlhan6. ÖZET Araştırmada, 50* si 46,XX ve 50'si 46, XY karyotipinde toplam 100 bi reyin MC Coy 5A besiortamında lenfosit hücre kültürleri yapılmıştır. Kül tür ortamlarına, bazı talasemi grupları ve orak hücreli anemi tedavisinde kullanılan ve bir baz analogu olan 5-Azo deoksisitidin (5-Azo dC), beş de ğişik sürede (2,12,24,48 ve 72 saat) ve 10"8-10-2 M derişimlerinde eklen miştir. Ajan aynı zamanda bir metilasyon inhibitörüdür. 5-Azo dC'nin yanısıra birbaşka baz analogu olan 5-Bromo 2'-deoksiu- ridin'in [(5-BrdU) ve (Timin analogu)] 10 ng/ml'lik sabit bir dozu kontrol ve deney grubu kültür ortamlarına üç farklı sürede (24, 48 ve 72 saat) eklen miştir. Standart preparasyon yöntemi (makrometod) kullanılarak preparatlar hazırlanmış ve metafaz kromozomlarının detaylı analizleri için G (GTG), C (CBG) ve BrdU bant tekniklerinden yararlanılmıştır. Yapılan inceleme sonunda, ajanın DNA'yı demetile ettiği saptanmış tır. Bu durum, kromozom yapılarında saptanan dekondansasyon (kromozom 1, 16, ve X), segmentasyon ve dekonfigürasyon (yaklaşık bütün kromo zomlarda) varlığından anlaşılmıştır. Kromozomların fakültatif, sentromerik heterokro matin ve satellit tip DNA bölgelerinde, dekondansasyon ve ankondansasyon belirlenmiştir. Bu nun yaraşıra belli grup kromozomlarda kümeleşme ile birlikte D ve G grup kromozomlarda satellit tip DNA 'ya bağlı asosiasyon artışı görülmüştür. Bunlar, telomerik DNA asosiasyonu ve jukstasentromerik asosiasyonlar o- larak tanımlanmıştır. 5- Azo dC'nin sitidinle yer değiştirerek kromozom-kromatin kondan- sasyonunu bozduğu gözlenmiştir. Kromozomun ajan ile yer değiştiren siti- dince zengin bölgelerinin, hipometilasyona bağlı olarak kondansasyonunu tamamlayamadıkları ve dekondanse formda kaldıkları saptanmıştır. Metilasyon, kromatinin kromozom şeklinde paketlenmesinde etkili bir mekanizmadır. Ajanın insan kromozomlarında en etkili dozunun 10" ^M ve sürenin ise 72 saatlik uygulama olduğu bulunmuştur.Öğe Analysis of Ki-ras exon 2 gene mutations in 3-methylcholanthrene and butylated hydroxytoluene-induced rat lung tissues(2008) Polat, Fikriye; Özdemir, Öztürk; Elagöz, Şahende3-Methylcholanthrene (MCA) is a polycyclic aromatic hydrocarbon and potent carcinogenic agent that is often used in experimental cancer studies. Butylated hydroxytoluene (BHT) has been widely used for many years as an antioxidant to preserve and stabilize the freshness, nutritional value, flavor, and color of foods. The aim of the present study was to investigate the role of the application of MCA and BHT in the development of lung cancer, and to detect any mutation in the Ki-ras gene exon 2. Rats were killed by cervical dislocation 26 weeks after the last BHT injection and lung tissues were surgically removed. Lung tissues of the control and experiment rat groups were examined for point mutations in the exon 2 of the Ki-ras by PCR based SSCP analysis. No point mutation was observed in the Ki-ras gene exon 2. © TÜBİTAK.Öğe Associations between common 3435 C>T variants of the multi-drug resistance [MDR-1 (ABCB1)] gene and abdominal aortic aneurysm: A pilot study(2011) Manduz, Şinasi; Katrancıoğlu, Nurkay; Karahan, Oğuz; Yılmaz, Mehmet Birhan; Özdemir, Öztürk; Berkan, ÖcalAmaç: Bu çalışmada, inflamatuvar süreç ve oksidatif strese karşı rol oynadığı ileri sürülen C3435T multi drug rezistans-1 (MDR-1) gen polimorfizminin AAA'daki etkisi ortaya konuldu. Çalışma planı: Bu çalışmada servisimizde AAA tanısı konulduktan sonra ameliyat edilen 58 hasta (41 erkek, 17 kadın; ort. yaş 62.9±6.6) ile abdominal bilgisayarlı tomografi taramasında aort çapları normal olarak ölçülen 58 sağlıklı kişinin (38 erkek, 20 kadın; ort. yaş 58.8±11.6 yıl) periferik kan örneklerinde MDR gen mutasyonu tarandı.Bulgular: MDR-1 C3435T geni CT varyant (x2=5,80, p=0.016) ve MDR-1 C3435T geni TT varyant (x2=11.47 p=0.001) polimorfizmleri AAA'lı hastalarda istatistiksel olarak anlamlı bulundu (p<0.05). Demografik bulgular gruplar arasında benzerdi.Sonuç: Elde edilen ilk sonuçlar MDR-1 geni T alleli polimorfizminin AAA ile ilişkili olduğunu düşündürmektedir. Bu ve benzeri moleküler çalışmaların AAA etyolojisinin anlaşılmasında gelecekte yapılacak çalışmalara öncü olacağı kanaatindeyiz.Öğe Chemokine receptor 5 ?32 gene polymorphism and abdominal aortic aneurysms(2010) Aydin, Murat; Katrancio?lu, Nurkay; Manduz, Şinasi; Atahan, Erhan; Karahan, O?uz; Özdemir, Öztürk; Berkan, ÖcalBackground: In this study, we aimed to investigate the relationship between abdominal aortic aneurysm (AAA) and chemokine receptor 5 ?32 (CCR5) gene polymorphism as a risk factor. Methods: Fifty-eight patients (41 males, 17 females; mean age 62.9±6.5 years; range 45 to 78 years) operated on our clinic between May 2008 and March 2009 with the diagnosis of AAA, and 58 healthy volunteers (38 males, 20 females; mean age 58.8±11.6 years; range 30 to 79 years) with normal aortic diameters measured by computed tomography were included in this study. Thirty-two base p deletions in the CCR5 gene were screened after obtaining genomic DNAs from peripheral blood samples of the patients. Results: When the groups were compared with the predisposing risk factors for the development of AAA, no significant difference was observed (p>0.05). Eleven patients (19.0%) had heterozygote CCR5 gene mutation in the AAA group, however, only one patient (1.7%) had heterozygote CCR5 gene mutation in the control group. While the CCR5 homozygote was normal in 47 (81.0%) patients, the CCR5 homozygote was normal in 57 (98.3%) volunteers in the control group. Chemokine receptor 5 ?32 heterozygote gene mutation was significantly higher in the AAA group. (p=0.004). Conclusion: Consequently, a relationship between CCR5 gene polymorphism and AAA was demonstrated in this study. We think that hereditary factors considered between unchanged etiologic factors play a role in the development of AAA and we believe that AAA can be treated before serious complications occur with frequent clinical check ups in people with hereditary predisposition.Öğe Detection of genotoxic effect of potassium permanganate by using in vitro micronucleus assay(Sivas Cumhuriyet University, 2020) Gencer, Gökhan; Özdemir, Levent; Özdemir, ÖztürkObjective: Hydrogen peroxide is a bleaching agent accepted as safe for environment and used for producing cotton fabrics. This process requires alkaline medium, stabilizer and high temperature. For this purpose, it is used extensively in the textile industry. Potassium permanganate is a widely used agent in our country as it is easier to obtain compared to other oxidizing agents. In this study, we investigated whether occupational exposure to potassium permanganate causes genotoxic effect or not.Method: This cross-sectional study was carried out in a denim factory located in the Organized Industrial Zone in Sivas. The total number of employees was 600 in the factory including 50 workers in the office and 550 workers in the production department.The study group was consisted of 32 healthy males, non-smokers, without chronic diseases and infections. They had been working at least 2 years in denim bleaching unit. The control group was consisted of 30 healthy male volunteers, at similar age, non-smokers, working in administrative or desk jobs in the same factory, with little or no risk of chemical exposure. Workers who had an infection in the last month and who were on medication were excluded from the study. In Vitro Micronucleus Test was used to determine the genotoxic effect of potassium permanganate. The venous blood samples taken from the workers and controls was cultured in laboratory conditions according to Fenech’s method with minor modifications. Evaluation of slides was carried out according to Fenech’s microscopic survey criteria at ×1000 magnification. The frequencies of the total number of micronuclei in the lymphocytes (MNL) and bi-nucleated cells with the micronuclei (BNMN) were determined. In addition, to determine the frequencies of nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs), 1000 nucleated cells with well-preserved cytoplasm were also evaluated. Student’s T-test was used for statistical evaluation. Results: When groups were compared, the micronucleus frequency was detected as about twice high in study group. Statistically significant difference was detected between the study and control group (p<0.05). The nuclear bud frequency was significantly higher in study groups than in controls (p<0.05). Although the number of nucleoplasmic bridges were higher in study group, this difference was not statistically significant (p>0.05).Conclusions: Indicators of genotoxic effect were higher in the group with potassium permanganate exposure than the control group. High micronucleus and nuclear bud frequencies in the study group may be indicative of genotoxicity. It may be thought that clastogenic and mutagenic risk increases for employees.Keywords: Potassium permanganate, micronucleus, nucleoplasmic bridge, nuclear bud, genotoxicityÖğe Genetic constitution analysis of idiopathic sudden hearing loss(İstanbul Üniversitesi, 2010) Bora, Adem; Altuntaş, Emine Elif; Özdemir, Öztürk; Uysal, İsmail Önder; Müderris, SuphiObjectives: The purpose of this research is to understand the etiology of sudden hearing loss due to genetic factors in Turkish people. Determination of these genetic factors and better understanding of molecular pathogenesis may guide more realistic planning and treatment recommendations. Patients and Methods: Forty patients Group 1; 19 males, 21 females; mean age 37.9±15.6 years; range 9 to 76 years who presented with sudden hearing loss to the Ear, Nose and Throat Clinic of Medical Faculty Hospital of Cumhuriyet University between January 2008 and June 2009, and were diagnosed with sudden hearing loss through history, physical examination and review of audiometric findings, and 20 healthy volunteers Group 2; 14 males, 6 females; mean age 31.7±4.4 years; range 24 to 43 years for the control group were included in this study. All Patients were evaluated by the genetic clinic for the GJB2, GJB3, GJB6 and WFS1 gene using multiplex ligation-dependent probe amplification MLPA method mutation analysis. Results: No difference was found in the peripheral blood sample analyses of the two groups at WFS1 exon 8 and connexin 26, 30 and 31 gene zones using the MLPA method with respect to heterozygous mutation p=0.291, p>0.05 . In four patients in group 1 heterozygous mutation was detected at the target gene zone. Heterozygous mutation was in the WFS1 exon 8 zone in two patients; and in the WFS1 exon 1 zone in other two patients. Conclusion: Sudden hearing loss studies in the future should include connexin 26, connexin 30 and other gene mutations that may affect the function of the gap-junction located in the region of the cochlea stria vascularis stV , basal membrane BM , spiral limbus Li and spiral ligament SL . These studies should be performed on larger series, and should include family members of patients with sudden hearing loss.Öğe Karyometric analysis of nasal polyps(İstanbul Üniversitesi, 2006) Yıldırım, Altan; Özdemir, Öztürk; Gül, Eylem; Yıldırım, Beyazıt; Elagöz, Şahende; Kunt, TanferObjectives: Nuclear characteristics of epithelial cells in nasal polyps were analyzed.Patients and Methods: The slides of 35 patients who undervvent surgery for nasal polyposis and 18 slides of normal mucosa were examined. The specimens were stained with hematoxylineosin. Tissue sections were analyzed by a CCD camera and the Karyotype Program of Human Cytogenetics Nomenclature System on a Macintosh Computer OS 9 . Fifty epithelial cells in each slide were randomly selected and transferred to the automated karyotyping system and photographed. The diameters of the nuclei were mea- sured and some peculiar nuclear features were exam- ined, including unsmooth appearance of the nuclear membrane, anaphase-bridge, and binucleation.Results: There were significant differences between nasal polyp and normal mucosa groups in terms of nuclear diameter, perimeter, and volüme, with ali variables being greater in the nasal polyp group p=0.001 . Unusual nuclear features differed significantly unsmooth nuclear membrane appearance, pÖğe MEME KANSERLİ HASTALAR VE BİRİNCİ DERECE YAKINLARINDA PERİFERAL TRANSKRİPTOM PROFİLLERİNİN ARAŞTIRILMASI(2019) Erdiş, Eda; Yücel, Birsen; Özdemir, ÖztürkAmaç: Meme kanserinde gen ekspresyon profillerinin kantitatif olarak ölçümü klinik düzeyde önemli bir araçolma potansiyeline sahiptir. Bu çalışmada, meme kanserli hastalar, bu hastaların birinci derece yakınlarınınperiferal transkriptom profilleri sağlıklı kontrol grubu da dahil edilerek kantitatif olarak karşılaştırılması hedeflenmiştir.Materyal-metod: Çalışma popülasyonu, cerrahi ve sonrasında kemoterapi ile tedavi edilmiş olup, radyoterapi için kliniğimize başvuran 30 meme kanserli hasta (aile öyküsü olmayan sporadik vakalar), bu hastaların30 kişilik birinci derece yakınları (kız kardeş veya kız çocukları) ve 30 kişilik sağlıklı, aile öyküsü negatif olanbireylerden oluşmaktadır. Total genomik RNA, her üç grupta da periferal kandan ayrılmıştır ve gen ekspresyon profilleri niceliksel olarak belirlenmiştir. Çalışmamızda 36 gen ürünü değerlendirilmiştir. Ancak sadeceER, HER2, p53, GATA–3, GRB–7, EGFR, MYC, BCL–2, VEGF gibi 9 genin ekspresyon profilleri kantitatif olarakölçülebilmiştir.Bulgular: Kantitatif olarak ölçülen 9 gen profili, her 3 grup için karşılaştırıldığında, gruplar arasında farksaptanmamıştır (p<0.050). Ancak HER2, GATA3, GRB-7 ve EGFR’nin gen ekspresyon profilinin ortanca kantitatif değerleri hasta grubunda, hasta yakını ve meme kanseri olmayan bireylere göre anlamlı daha yüksekbulunmuştur (p<0.050).Sonuç: HER2, GATA3, GRB-7 ve EGFR’nin gen profilinin kantitatif değerleri periferik kandan ölçülmüş olup,hasta grubunda, hasta yakını ve meme kanseri olmayan bireylere göre anlamlı daha yüksek bulunmuştur.Öğe Mitochondrial DNA variation in turkish brown trout (Salmo trutta L., 1758) populations(2004) Bardakcı, Fevzi; Değerli, Naci; Başıbüyük, Hüseyin Hasan; Özdemir, Öztürk[Abstract Not Available]Öğe Populasyonda kromozom polimorfizm sıklığı ve beraberindeki genetik stigmatlar(Cumhuriyet Üniversitesi, 1989) Özdemir, Öztürk; Sezgin, İlhan60 ÖZET Bu araştırmada, toplumumuzun kromozom polimor- fizm sıklığını saptamak amacı ile yaş ortalaması 19 olan (17-40) sağlıklı 100 kişide kromozom analizi yapılmıştır. Elde edilen bulgulara "iki grup arasın daki farkın önemlilik testi" uygulanmış ve istatis tiksel olarak değerlendirilmiştir. Tüm kromozomlar ele alınarak, yapılarında po- limorfizra gösterenler Satellit,Sentromer,Yq Hacmi ve Frajil yanlar bakımından incelenmiş ve beraberinde ki bazı genetik stigmatlar değerlendirilmiştir. Bu yapılar bakımından toplumumuz için ortala ma kromozom polimorfizm sıklığı % 25.5, stigmatlarda ise % 30 bulunmuştur. Bunlardan Satellit Polimorfiz- rai % 71.6 (% 74 ü sayı ve hacim, % 26 sı pozisyon polimorf izmi), Yq Hacim Polimorf izmi % 10.6 (genel polimorfik yapıların % 6.8 i), Sentromer Polimorf iz mi % 11 ve Frajil Yan Polimorf izmi % 10.8 olarak saptanmıştır. Ayrıca % 11 oranında Disentrik Yapı ve % 19 oranında Satellit Saperasyon anomalisine rastlanmıştır. Belirlenen bu değerler İstatistiksel olarak önemli bulunmuştur (P<0.05).Öğe The differences of prothrombotic gene polymorphism in deep venous thrombosis cases with lung cancer(2010) Erdiş, Eda; Karahan, O?uz; Manduz, Şinasi; Özdemir, ÖztürkObjective: Deep vein thrombosis (DVT) is a common and troubled disease that can result in higher rates of morbidity and mortality if not diagnosed and treated carefully. The basic hypothesis on etiology of DVT is that most patients who complain idiopathic DVT have a genetic predisposition, which remains subclinical until an extra stress occurs. Cancer is another important risk factor for DVT. Cancer and its treatment can affect all three arms of Virchow's classical triad of causation of thromboembolic disease: alteration in blood flow (by creating mass), damage of endothelial cells, and trigger of procoagulants. In this study we aimed to observe the differences of prothrombotic gene polymorphisms in DVT cases with lung cancer versus DVT cases without cancer. Material and Methods: All cases with DVT were evaluated in terms of cancer. Cases were divided in two groups, the first with 33 patients that had DVT with lung cancer and the second with 63 patients that had DVT without cancer. We studied prothrombotic gene profile in each two group. Therefore we detected the prevalence of prothrombin G20210A, factor V G1691A (factor V Leiden), methylenetetrahydrofolate reductase (MTHFR) C677T and MTHFR A1298C, angiotensin converting enzyme (ACE), plasminogen activator inhibitor-1 (PAI-1), Glycoprotein IIIa gene polymorphisms, which are known cause thrombosis in our study groups. Results: We found that homozygote gene polymorphisms of PAI-1 (PAI-1 4G/4G) were significantly higher in cancer patients with DVT (x2=9.41 p=0.002). Furthermore, no significant difference was observed between genotypes for prothrombin G20210A, factor V Leiden, MTHFR C677T and MTHFR A1298C, ACE, Glycoprotein IIIa gene polymorphisms. Conclusion: The gene polymorphism of homozygote PAI-1 (PAI-1 4G/4G) may be an important risk factor for DVT patients with lung cancer. However detection of no significant difference between two groups in terms of other prothrombotic gene polymorphisms suggests other genes together with non-genetic factors. Copyright © 2010 by Türkiye Klinikleri.Öğe Treatment of ethylnitrosourea induced lymphocyte hyperproliferation by DNA hypomethylation in the rat colon(2002) Özdemir, Öztürk; Bulut, Hüseyin Eray; Korkmaz, Mehmet; E?ilmez, Reyhan; Atalay, AtillaN-ethyl-N-nitrosourea (ENU) is a potential carcinogenic agent commonly used in industry, and it may cause an uncontrollable cell proliferation and eventually tumourgenesis. On the other hand, the hypomethilation of DNA by 5-aza-2?-deoxycytidine is the best known anti-tumoural mechanism used for the treatment of leukemia. Therefore the present study aimed to find out the possible healing effects of 5-aza-2?-deoxycytidine on lymphocyte hyperproliferation in the rat colon through the above mentioned DNA hypomethylation mechanism. Rats were injected with 300 mg/kg body weight ENU (i.p.) in order to induce tumour development. Following 45 weeks when the tumourgenesis was proved visually, animals were treated with 5-aza-2?-deoxycytidine 100 ?g/100g body weight twice a week intraperitoneally for 15 weeks. After the experimental procedure, all animals were sacrificed and colonal tissues were obtained. Tissues were processed for light and electron microscopy. While no colonal tumour development was observed in the control group, an extensive tumour development was seen in the subcutaneous region in the high dose ENU treated group. The light and electron microscopical examination of the rat colonal tissue revealed a lymphocyte hyperproliferation and invasion in the submucosal region, an increased number of polimorphonuclear leukocytes (PMNLs) and occasional epithelial lesions. On the other hand, the evaluation of the 5-aza-2?-deoxycytidine treatment group rat colon demonstrated features similar to those seen in the control and PEG treated groups indicating a possible anti-neoplastic effect of 5-aza-2?-deoxycytidine via DNA hypomethylation.
