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    A histopathological and clinical assessment on the effectof microneedling on the autologous platelet-rich plasma inthe experimental wound healing model
    (2021) Özkaya, Neşe Kurt; İnan, Zeynep Deniz Şahin; Zereyak, Umut; Yılmaz, Sarper
    Aim: Platelet Rich Plasma (PRP) is a method used in dentistry and aesthetic applications in order to accelerate tissue healing. The purpose of this study is to assess the effect of PRP on accelerating wound healing and the contribution of microneedling application, i.e. multiple injection during PRP, and the volume effect of the PRP material to healing.Materials and Methods: In the study, 16 5-month-old male white New Zealand rabbits with an average weight of 2.7-3.0 kg were used. The four wounds were made on the back of each rabbit, and PRP, Normal saline(NS), microneedling(MN), and Sham procedures applied. Eight rabbits were sacrificed by taking their tissues for histological assessment at the end of 14 days. Epithelization, VEGF expression, angiogenesis, inflammatory cell, fibroblast, reticular fiber, and collagen parameters were evaluated. Eight rabbits were followed up for macroscopic healing. Result: The PRP procedure decreased the healing duration and wound area compared to other procedures(p<0.05). The NS procedure decreased the healing duration compared to the Sham procedure and provided a significant decrease in wound area compared to the MN and Sham procedures(p<0.05). Although the MN procedure decreased the wound area compared the Sham procedure, this difference was not statistically significant(p>0.05). PRP procedure increased staining scores and densities of epithelization, VEGF expression, angiogenesis compared to Sham procedure(p<0.05). PRP procedure increased staining score of collagen compared to Sham and NS procedures and staining density of collagen compared to other procedures(p<0.05). MN procedure decreased staining density of inflammatory cell compared to Sham procedure(p<0.05). Conclusion: The evaluation of simultaneous microneedling and PRP procedure on the 14th day of wound healing did not provide more positive synergistic effects on healing. In order to reduce the volume effect of the PRP amount given, the amount of PRP applied at each point should be as low as possible.
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    Effect of the wrapping of experimentally induced ischemic colonic anastomoses with the amniotic membrane on anastomosis safety in rats.
    (2018) Soylu, Sinan; İnan, Zeynep Deniz Şahin
    Objective: Colonic anastomotic leakage represents a complication with high mortality and morbidity. In the presentstudy, it was aimed to examine the effect of the amniotic membrane on the healing of ischemic colonic anastomosis.Method: 25 pregnant Wistar albino rats aged 19-21 days were randomly divided into 5 groups. With midlinelaparotomy, pregnancies were terminated, and fresh amniotic membranes were obtained. In Group 1 Sham (S), noprocedure except for laparotomy was performed. In Group 2 of colonic anastomosis (A), the descending colon wascompletely transected and anastomosed. In Group 3 of amniotic anastomosis (AA), the anastomosis line was fixed bybeing wrapped with the fresh amniotic membrane. In Group 4 of colonic ischemic anastomosis (IA), the marginalarteries were ligated after descending colon transection. Ischemia was induced, and anastomosis was performed. InGroup 5 of colonic ischemic anastomosis wrapped with the amniotic membrane (AIA), after ischemic colonicanastomosis was performed, the anastomosis line was fixed by being wrapped with the amniotic membrane. On thepostoperative 7th day, rats were sacrificed, and their anastomotic bursting pressures were measured. For histologicalexamination, the colon segment containing the anastomosis line was removed. Hematoxylin-eosin and Van Giesontrichrome stained specimens were histologically evaluated and scored according to the Ehrlich-Hunt model.Results: Anastomotic bursting pressures were measured in group S as the highest, in AA, AIA, A in a decreasing orderand in group IA as the lowest. The difference between groups S and AA was statistically insignificant in the pairedcomparison of groups according to their bursting pressure values (p=0.222). When the other pairs were compared, thedifference between the groups was calculated as significant (p< 0.05). According to the Ehrlich-Hunt model, the totalscore was the highest in AIA group, decreased in AA, IA, A groups in a decreasing order, and was the lowest in groupS. A paired comparison for total scores between the groups was statistically insignificant between groups S and A(p=0.222). Other paired comparisons were statistically significant (p< 0.05).Conclusions: Wrapping the colon anastomosis line with the amniotic membrane gave positive results both in theischemic colon as well as in the non-ischemic colon in terms of bursting pressure and histological anastomosis safety.
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    The Effect of Two Different Botulinum Neurotoxin A On The Cortical Neuron Cells In Terms of Apoptosis and MMP 2, MMP 7, and MMP9 Localizations
    (2023) İnan, Zeynep Deniz Şahin; Polat, Zübeyda Akın; Hamutoğlu, Rasim
    This study aimed to associate the possible cytotoxic and apoptotic effects of Botox (Allergan) and Dysport (Ipsen) and immunolocalization of matrix metalloproteinase (MMP) proteins with HCN2 cortical neuron cell line. Accordingly, cytotoxic potentials of Botox and Dysport were determined on different concentrations. Then, the apoptosis rates of these cells were evaluated by TUNEL method. MMP2, MMP7, and MMP9 proteins were also visualized using immunofluorescence method. There was a significant difference in cytotoxicity between those treated with 3.2, 6.4, and 12.8IU compared with the control. The Dysport 12.8 IU group was statistically more toxic than Botox group at the same concentration. Therefore, the number of apoptotic cells increased from 0.1 IU in Botox and 0.01 IU in Dysport groups compared to the control. The number of apoptotic cells was significantly higher in Dysport group at 1.6, 3.2, 6.4, and 12.8 IU concentrations than in Botox group. It was determined that MMPs increased gradually at the concentrations where the number of apoptotic cells was highest compared to the control group. As a result, we consider that it may be necessary to deal with the dose adjustment in Botox and Dysport applications, together with detailed studies to be carried out in the future.