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    Anticancer, Antioxidant, Antimicrobial and Enzyme Inhibitory Activities of Inula aucheriana
    (Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Guclu, Gulsen; Ergul, Merve; Ucar, Esra; Eruygur, Nuraniye; Atas, Mehmet; Akpulat, Huseyin Askin
    The Inula aucheriana, of the Asteraceae family, is widespread in Turkey. The aim of this study was to investigate different biological properties and thereby reveal the pharmacological potential of this plant, which is already known to be used in the treatment of various diseases,. Following the preparation of 80% ethanol extract from I. aucheriana, qualitative and quantitative methods were used to investigate the chemical composition (Q-TOF analysis), antioxidant (spectrophotometric analysis), inhibitory enzyme (Ellman's method), antimicrobial (MIC concentration value), and anti-cancer (XTT analysis) activities. The results showed that 80% ethanol extract from I. aucheriana was a potent antioxidant, with anti-cancer and enzyme inhibitor effects. In the chemical composition analysis, the primary compound of the extract was determined to be luteolin (32.55%). I. aucheriana extract was seen to have AChE and BChE inhibition, , and when compared with the reference drug, the extract was determined to have an inhibitory effect an enzyme called alpha-glucosidase. Besides, relatively high tyrosinase enzyme inhibition was also detected. The extract significantly showed antiproliferative activity on the MDA-MB-231 cells at 0.0625 mg/mL and higher concentrations,for 24 hours in a a dose-dependent manner.This study is the first to evaluate enzyme inhibitory effect and antioxidant activity of I. aucheriana.
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    Chemical composition and antioxidant activity of the essential oil and various extracts of Inula graveolens (L.) Desf.
    (Kilis 7 Aralık Üniversitesi, 2021) Akpulat, Huseyin Askin; Sahınler, Saliha Seyma
    In this study, chemical composition and in vitro antioxidant activity potential of the essential oil and various extracts of Inula graveolens (L.) Desf. were evaluated. While identifying the phytochemical composition of the essential oil and extract, GC-MS analyses were used. Chromatographic analysis of the essential oil resulted in identifying twenty compounds representing 99.5% of the total oil. Main constituents of the oil were determined as bornyl acetate (68.5%), borneol (7.7%), camphene (4.6%), epi-alpha-cadinol (4.0%) and eicosane (3.2%), respectively. Antioxidant activity was determined using four complementary test systems named beta-carotene/linoleic acid, DPPH free radical scavenging, reducing power, and chelating effect. A strong correlation between the antioxidant activity and phenolic acid contents of the samples was determined. The methanol extract was the most active one in all tested systems. The weakest activity was exhibited by chloroform extract. While methanol extract showed 88.34%, 91.38, and 63.43 activities in beta-carotene bleaching, DPPH radical scavenging, and chelating effect tests, respectively, the absorbance value in reducing power assay was measured as 0.273 nm.
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    Evaluation of Various Biological Activities of Endemic Sideritis libanotica Extracts
    (Horticulture and Forestry Society from Transylvania, 2019) Atas, Mehmet; Eruygur, Nuraniye; Sozmen, Fazli; Ergul, Mustafa; Ergul, Merve; Akpulat, Huseyin Askin; Ucar, Esra
    In recent years, using of plants to develop combine therapies for treatment of certain diseases such as cancer, Alzheimer's disease, and diabetes mellitus is extremely plausible approach. Perhaps, the definitive treatment of some of these diseases is hidden in the complex content of a plant in nature. Thus, in the present study, we decided to determine the biological activities of methanol and water extracts of the endemic Sideritis libanotica. To the best of our knowledge, the present study is the first investigation on antioxidant, enzyme inhibitory activity, antimicrobial activity, and in vitro cytotoxicity of S. libanotica. According to results, while the methanol extract shows better anti-cholinesterase, ?-glucosidase, and ?-amylase inhibition activity and cytotoxicity than the water extract, the tyrosinase inhibitory activity of the water extract was found to be better than the methanol extract. This study provides valuable information on how the biological activity of endemic S. libanotica changes in different solvent extractions such as water and methanol. © 2019, Horticulture and Forestry Society from Transylvania. All rights reserved.
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    In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium
    (SPRINGER, 2019) Eruygur, Nuraniye; Ucar, Esra; Akpulat, Huseyin Askin; Shahsavari, Keyhan; Safavi, Seyed Mehdi; Kahrizi, Danial
    Hypericum lydium Boiss. is a perennial plant of the Hypericaceae family, which has been used in particular to treat depression. The aim of this study was to determine in vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (-glucosidase/-amylase) and Tyrosinase inhibitor activity of methanol and water extracts of H. lydium. Also, gene expression has been evaluated in the shoot and root by microarray technology. So, in general, the purpose of this study is to study the active molecules such as antioxidant, antimicrobial, antidiabetic, enzymes and genes in the plant, which is the first to be reported. The experiments were conducted in a completely randomized design with three replications. In addition, gene expression was compared in the shoot and root parts. Expression profiling was carried out by microarrays. According to the results, the highest chemical components were determined in methanol extract rather than water extract. There was a difference between the obtained components. While the highest antioxidant activity was determined from the methanol extract of plant herbs for DPPH Free Radical Scavenging Activity, antioxidant activity was the same in both methanol and water extracts using the ABTS method. The methanol extract demonstrated stronger anticholinesterase (AChE and BChE) and -amylase inhibition activity. This study was complemented by the detection of antioxidant activity and some enzyme inhibition activity in the methanol extract. Microarray showed 10,784 genes had significantly different expression in root and shoot. There was a positive effect of methanol extract in respect of different activities compared to the water extract. Gene expression showed that the number of expressed genes in the root was greater than the shoot.
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    IN VITRO ANTIOXIDANT, ANTIMICROBIAL, ANTICANCER ACTIVITIES ASSESSMENT OF THYMUS PECTINATUS, SCREENING OF ENZYME INHIBITORY
    (Parlar Scientific Publications (P S P), 2021) Ergul, Merve; Guclu, Gulsen; Atas, Mehmet; Cetin, Metin Durmus; Eruygur, Nuraniye; Ucar, Esra; Akpulat, Huseyin Askin
    According to the data obtained in this study, Thymus pectinatus has antioxidant and enzyme inhibitory activities while has not showed antimicrobial activities. The results of the current study provide valuable information, showing that the major component of the water extract of T. pectinatus is 1,3-Propanediol, 2 -methyl-, dipropanoate (59.34 %). This is the first investigation of the antioxidant and enzyme inhibitory activity of T. pectinatus. The results showed that the water extract had high antioxidant, anti-amylase, and anticancer effects, and a higher total phenolic content. Therefore, further phytochemical and bioactivity-guided isolation of T. pectinatus water extract could be carried out to identify the active compounds.
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    In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas
    (Mdpi, 2019) Ustuner, Oya; Anlas, Ceren; Bakirel, Tulay; Ustun-Alkan, Fulya; Sigirci, Belgi Diren; Ak, Seyyal; Akpulat, Huseyin Askin
    Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H2O2) by increasing cell viability compared to only H2O2-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds.
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    A new record for the flora of Turkey: Sorbus caucasica var. caucasica (Rosaceae)
    (TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2012) Eminagaoglu, Ozgur; Ozkaya, Mimar Sinan; Akpulat, Huseyin Askin
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    The pharmacological properties of Gypsophila eriocalyx : The endemic medicinal plant of northern central Turkey
    (Elsevier, 2024) Inanir, Merve; Ucar, Esra; Tuzun, Burak; Eruygur, Nuraniye; Atas, Mehmet; Akpulat, Huseyin Askin
    The aim of this study is to evaluate and compare the biological properties of different extracts (methanol, ethanol, and water) obtained from Gypsophila eriocalyx (G. eriocalyx), a medicinal plant traditionally used in Turkey. The components of different extracts were defined using the GC-MS method. The effects of G. eriocalyx extracts on cell proliferation, apoptosis, and cell cycle arrest in MDA-MB-231 breast cancer as well as in vitro antioxidant, enzyme inhibition, and antimicrobial activities were investigated. In accordance with the results obtained, although ethanol and methanol extracts of G. eriocalyx show higher antioxidant activity than G. eriocalyx water extract, enzyme inhibition activities of the extracts were not found to be significant compared to the reference drug. The methanol and ethanol extract of G. eriocalyx exhibited moderate antimicrobial activity against Staphylococcus aureus and methanol extract showed significant antimicrobial activity against Bacillus cereus. In addition, both extracts significantly inhibited cell viability in a dose-dependent manner in breast cancer cells. The cell growth inhibition by methanol and ethanol extracts induced S phase cell-cycle arrest and apoptosis in MDA-MB-231 cells. Lastly, in order to compare the activities of the chemicals found in Gypsophila eriocalyx plant extract, their activities against various proteins that are breast cancer protein (PDB ID:1A52 and 1JNX), antioxidant protein (PDB ID: 1HD2), AChE enzyme protein (PDB ID: 4M0E), BChE enzyme protein (PDB ID: 5NN0), and Escherichia coli protein (PDB ID: 4PRV)were compared. Then, ADME/T analysis calculations were made to examine the effects of molecules with high activity on human metabolism. Eventually, G. eriocalyx is thought to be a potent therapeutic herb that can be considered as an alternative and functional therapy for the management of diseases of a progressive nature related to oxidative damage such as infection, diabetes, cancer, and Alzheimer's disease.