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  • Küçük Resim
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    Experimental and theoretical insights about the effect of some newly designed azomethine group?contained macroheterocycles on oxidative stress and DNA repair gene profiles in neuroblastoma cell lines
    (Elsevier, 2023/8/5) Cinar, Gulcihan; Agbektas, Tugba; Huseynzada, Alakbar; Aliyeva, Gunel; Aghayev, Mirjavid; Hasanova, Ulviyya; Kaya, Savas; Chtita, Samir; Nour, Hassan; Tas, Ayca; Silig, Yavuz
    We report herein the synthesis of new azomethine group containing forty-, sixty-eight- and seventy- two-membered macroheterocyclic compounds and the investigation of their activity against the neurob- lastoma cell line. The synthesis of targeted compounds was done by condensation of dialdehydes with polyamines and their structures were investigated by 1 H and 13 C NMR and MALDI MS methods. Anti- cancer activity of these newly synthesized molecules was studied in the neuroblastoma (SH-SY5Y) cell line at eight different concentrations (1–100 μg/ml) for 24 h, 48 hrs and 72 h by MTT method. In ad- dition to it, oxidative stress (GPX1, PRDX1, CAT, SOD1, NQO1) and DNA repair (ATR, ERCC1, CDKN1A, PRKDC) gene profiles were also investigated by RT-PCR method. It was found that all synthesized compounds showed the highest activity after 72 h of incubation. PRDX1 gene expression in the case of all investi- gated compounds was found to be higher than the control group, whereas ABCB1 (MDR) gene expression increased only in the case of molecule 1. Results also demonstrate that the expression levels of GPX and SOD1 genes were high in the case of molecule 2, whereas molecule 1 manifested low expression levels of ERCC1, ATR, CDKN1A, PRKDC, GPX1, ABCB1(MDR), CAT, SOD1 and NQO1 genes. Along with experimen- tal studies, theoretical studies were also carried out. The String v11 program was used to determine the interaction of proteins involved in oxidative stress and DNA repair mechanisms with other proteins. The results of the molecular docking analysis were found to be in good agreement with the experiments.
  • Küçük Resim Yok
    Öğe
    Investigation of The Effect of B-108 Contaınıng Azomethin Group On DNA Repair Gene
    (Sivas Cumhuriyet University, 2022) Eğilmez, Elif; Zontul, Cemile; Huseynzada, Alakbar; Aliyeva, Gunel; Hasanova, Ulviyya; Taş, Ayça; Siliğ, Yavuz
    Objective: Cancer arises as a result of the failure of the mechanisms controlling normal division in a group of cells. It is known that some new synthesis compounds intended for use in cancer treatment have anti-fungal, anti-bacterial, anti-carcinogenic effects. In this study, it was aimed to apply the newly synthesized B-108 compound to the A-549 cell line and then to investigate the effect of this compound on the ERCC1 gene expression profile. Materials and Methods: Firstly, compound B-108 was synthesized in our study. Afterwards, this synthesized molecule was applied in eight different concentrations (1-100 μg/ml) in A-549 cell line and 3-(4,5-dimethylthiazol-2-yl)-2,5-yl for 24 hours, 48 hours and 72 hours. Anticancer activities were determined using diphenyltetrazolium bromide (MTT) method. Expression level of DNA repair gene (ERCC1) was determined using RT-PCR method. Results: As a result, it was determined that the molecule applied to the A-549 cell line showed the highest activity after 72 hours of incubation. It was observed that the ERCC1 gene expression of the molecule applied on lung cancer was lower than the control group. Discussion: Considering the current study results, low expression of ERCC1 shows that compound B-108 correlates with overall survival on lung cancer cells.