Yazar "Altundag, Huseyin" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim Yok
    Öğe
    Development of deep eutectic solvent extraction method for extraction of Zn2+ from powdered milk and baby formula samples and its determination by ICP-OES
    (Springer, 2020) Kurt, Aylin; Bagda, Esra; Altundag, Huseyin
    In the present study, the deep eutectic solvent-based extraction method has been developed for separation of Zn prior to ICP-OES analysis. Five different choline chloride-based deep eutectic solvents were prepared. The Zn extraction ability of DESs from milk powder and baby formulation samples which contain a high amount of milk protein was investigated. The parameters affecting the extraction efficiency such as the amount and type of solvent, extraction time and ambient temperature were optimized. Oxalic acid-based DES gave the highest yield and reproducible results and appropriate preparation criteria. It is concluded from the results that DES-oxalic can quantitatively extract Zn from milky samples. The analytical parameters such as linear calibration range, limit of detection (4.2 mu g L-1) and quantification (14.1 mu g L-1) were also calculated. The added-found method is successfully applied to ERM BD 150 standard reference material, and the recovery values and standard deviation values were found between the ranges of 79.4-97.21 mu g L-1 and 4.1-6.31 mu g L-1, respectively. The method was also applied to real samples, and the recovery values were found between 73 and 106% in the spiked samples.
  • Küçük Resim Yok
    Öğe
    Highly Simple Deep Eutectic Solvent Extraction of Manganese in Vegetable Samples Prior to Its ICP-OES Analysis
    (HUMANA PRESS INC, 2017) Bagda, Esra; Altundag, Huseyin; Soylak, Mustafa
    In the present work, simple and sensitive extraction methods for selective determination of manganese have been successfully developed. The methods were based on solubilization of manganese in deep eutectic solvent medium. Three deep eutectic solvents with choline chloride (vitamin B4) and tartaric/oxalic/citric acids have been prepared. Extraction parameters were optimized with using standard reference material (1573a tomato leaves). The quantitative recovery values were obtained with 1.25 g/L sample to deep eutectic solvent (DES) volume, at 95 A degrees C for 2 h. The limit of detection was found as 0.50, 0.34, and 1.23 mu g/L for DES/tartaric, DES/oxalic, and DES/citric acid, respectively. At optimum conditions, the analytical signal was linear for the range of 10-3000 mu g/L for all studied DESs with the correlation coefficient > 0.99. The extraction methods were applied to different real samples such as basil herb, spinach, dill, and cucumber barks. The known amount of manganese was spiked to samples, and good recovery results were obtained.
  • Küçük Resim Yok
    Öğe
    A Novel Selective Deep Eutectic Solvent Extraction Method for Versatile Determination of Copper in Sediment Samples by ICP-OES
    (SPRINGER, 2017) Bagda, Esra; Altundag, Huseyin; Tuzen, Mustafa; Soylak, Mustafa
    In the present study, a simple, mono step deep eutectic solvent (DES) extraction was developed for selective extraction of copper from sediment samples. The optimization of all experimental parameters, e.g. DES type, sample/DES ratio, contact time and temperature were performed with using BCR-280 R (lake sediment certified reference material). The limit of detection (LOD) and the limit of quantification (LOQ) were found as 1.2 and 3.97 A mu g L-1, respectively. The RSD of the procedure was 7.5%. The proposed extraction method was applied to river and lake sediments sampled from Serpincik, Celtek, KA +/- zA +/- lA +/- rmak (Fadl and Tecer region of the river), Sivas-Turkey.
  • Küçük Resim Yok
    Öğe
    The relationship between lymphocyte DNA damage, coronary artery disease, and blood trace elements
    (Oxford Univ Press, 2024) Erdogu, Ismail; Dural, Emrah; Altundag, Huseyin; Oymak, Tulay; Suzen, Halit Sinan
    Somatic DNA damage and causative factors (occupational exposures, foods, habits, etc.) are thought to contribute to the pathogenesis of atherosclerosis, although knowledge about their role in coronary artery disease (CAD) is still insufficient. This study aimed to determine the effects of lymphocyte-DNA damage and blood trace element concentrations on CAD. The single-cell alkaline comet was used in the measuring of the lymphocyte DNA damage in blood samples obtained from patients (n = 99) whose CAD grade was determined by the syntax score while the angiographic intervention was carried out. Blood trace element (n = 14) concentrations were monitored by the inductively coupled plasma-optical emission spectroscopy (ICP-OES) after microwave digestion. The relationship between the DNA damage frequencies of the participants and their syntax scores, blood trace element concentrations, and other demographic and clinic parameters were statistically analyzed. Significant correlations were detected between comet data and syntax score (r = 0.858, P < .001), age (r = 0.337, P < .001), blood-urea (r = 0.360, P < .001), creatinine (r = 0.388, P < .001), HbA1c (0.218, P < .05), ECG-QRS time (r = 0.286, P < .01), ECHO-EF (r = -0.377, P < .001), and platelet (r = -0.222, P < .05). The DNA damage frequencies of the groups formed according to their CAD scores were significantly different from the control group (P < .001) and also each other (P <= .01). Comet frequencies and CAD grades were found to be correlated with aging (P < .05). DNA damage frequency and syntax score values were significantly (P < .05) higher in males compared to females. Syntax scores were correlated with aging (r = 0.348, P < .01), ECHO-EF (r = 0.374, P < .001), blood-urea (r = 0.398, P < .001), creatinine (r = 0.433, P < .001), glucose (0.218, P < .05), and HbA1c (r = 0.200, P < .05). Significant correlations were observed between trace elements and demographic values, blood parameters, diseases, angio parameters, ECHO, and ECG parameters. It was observed that the concentrations of trace elements detected in the blood were 93.4% correlated with each other. Lymphocyte DNA damage is a strong biomarker for the atherosclerotic indicator of CAD. Aging is an effective factor both in the DNA damage frequency and CAD risk index. Creatinine and urea are factors that have the power to change the CAD risk index and DNA damage frequency. The higher DNA damage and CAD risk were monitored in males compared to females. The relationship between some biomarkers and blood trace element concentrations showed that further studies are needed to more accurately evaluate the relationship between trace elements, DNA damage frequencies, and CAD.