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Antioxidant-oxidant status in patients with hydatid cyst
(WALTER DE GRUYTER GMBH, 2012) Bakir, Sevtap; Celiksoz, Ali; Celik, V. Kenan; Sari, Ismail; Aydin, Huseyin
Objectives: To determine whether oxidative stress plays a role in the pathogenesis of hydatid cyst by examining levels of total antioxidant status, total oxidant status, oxidative stress index, and activities of the enzymes paraoxonase and arylesterase in patients with hydatid cyst. Methods: Thirty patients with hydatid cyst whom were positive for indirect hemagglutination and EchinococcusgranulosusIgGELISA tests were included in the patient group. Thirty-five healthy subjects that were negative for hydatid cyst by indirect hemagglutination and Echinococcus granulosus IgG ELISA tests, and did not have any parasites on stool inspection constituted the control group. Total antioxidant status, total oxidant status levels and paraoxonase, arylesterase activities were determined using commercial kits on an autoanalyzer. Oxidative stress index was calculated from a percent ratio of total peroxide level to the total antioxidant status level. Results: Serum total antioxidant status, total oxidant status and oxidative stress index levels of patients with hydatid cyst were significantly increased compared to the control group (p <0.05). Paraoxonase, and arylesterase activities of patients with hydatid cyst were significantly lower than the control group (p <0.05). Conclusion: Oxidative stress is increased in patients with hydatid cyst resulting in decreased paraoxonase and arylesterase activities. Increased oxidative stress might lead to increased tissue necrosis and inflammation. Therefore administration of an antioxidant therapy in addition to the routine treatment should be considered in this group of patients. It should also be noted that anti-atherosclerotic effect is reduced for a prolonged time secondary to decreased paraoxonase activity in cases of delayed diagnosis of hydatid cyst.
Apoptosis and its relation with clinical course in patients with Crimean-Congo hemorrhagic fever
(WILEY, 2019) Engin, Aynur; Aydin, Huseyin; Cinar, Ziynet; Buyuktuna, Seyit Ali; Bakir, Mehmet
Crimean-Congo hemorrhagic fever (CCHF) is a tick-mediated viral infection. Patients with CCHF may show various clinical presentations. The cause of this difference in the clinical course is not completely understood. Apoptosis is programmed cell death and plays an important role in regulating the immune system. Our knowledge of the role of apoptosis in CCHF disease is limited. We investigated the role of apoptosis and their relationship with the severity of the disease in CCHF. Thus, in 30 patients with CCHF and 30 healthy individuals, we analyzed the serum levels of cytochrome C, apoptotic protease activating factor-1 (Apaf 1), caspase 3, caspase 8, caspase 9, sFas, sFasL, perforin, granzyme B, and CK18 by enzyme-linked immunosorbent assay. This is the first study that research the serum levels of the mentioned apoptosis markers in adult patients with CCHF. We found that the serum levels of sFasL, cytochrome C, Apaf 1, caspase 3, caspase 8, caspase 9, perforin, granzyme B, and M30 were statistically significantly different in the acute phase of the disease compared with healthy individuals and patients in convalescent period. There was no association between the clinical severity of the disease and apoptosis markers. In conclusion, the results of our study suggested that the extrinsic and intrinsic apoptosis pathway play an important role in CCHF.
The Association between Diabetic Retinopathy and Levels of Ischemia-Modified Albumin, Total Thiol, Total Antioxidant Capacity, and Total Oxidative Stress in Serum and Aqueous Humor
(HINDAWI PUBLISHING CORPORATION, 2014) Kirboga, Kadir; Ozec, Ayse V.; Kosker, Mustafa; Dursun, Ayhan; Toker, Mustafa I.; Aydin, Huseyin; Erdogan, Haydar; Topalkara, Aysen; Arici, Mustafa K.
Purpose. To investigate the oxidant and antioxidant status of patients with type 2 diabetes mellitus and nonproliferative diabetic retinopathy (DRP). Methods. Forty-four patients who had cataract surgery were enrolled in the study. We included 22 patients with DRP in one group and 22 patients in the control group. Samples of aqueous humor and serum were taken from all patients. Serum and aqueous ischemia-modified albumin (IMA), total thiol, total antioxidant capacity (TAC), and total oxidative stress (TOS) levels were compared in two groups. Results. Median serum IMA levels were 44.80 absorbance units in the DRP group and 40.15 absorbance units in the control group (P = 0.031). Median serum total thiol levels in the DRP group were significantly less than those in the control group (3051.13 and 3910.12, resp., P = 0.004). Mean TOS levels in the serum were 2.93 +/- 0.19 in the DRP group and 2.61 +/- 0.26 in the control group (P = 0.039). The differences in mean total thiol, TAC, and TOS levels in the aqueous humor and mean TAC levels in the serum were not statistically significant. Conclusion. IMA, total thiol, and TOS levels in the serum might be useful markers in monitoring the risk of DRP development.
Association of left ventricular diastolic dysfunction with elevated NT-proBNP in type 2 diabetes mellitus patients with preserved ejection fraction: The supplemantary role of tissue doppler imaging parameters and NT-proBNP levels
(ELSEVIER IRELAND LTD, 2012) Ciftel, Sedat; Icagasioglu, Serhat; Yildiz, Gursel; Tekin, Gonca; Aydin, Huseyin
Background: Early diagnosis of cardiovascular disease in diabetic patients may be important to introduce treatment early. Echocardiography is a method used to show the ventricular functions. A ventricular hormone, BNP is used to identify the changes in the ventricular function in early period. NT-proBNP which is a more stable compound with a longer half-life is used in measurement of BNP. Methods: Left ventricular diastolic dysfunction (LVDD) was detected and NT-proBNP levels were measured in forty-four asymptomatic patients with ages of 30-70 and type 2 DM and control group consisted of 40 healthy individuals from the same age group. Results: NT-proBNP levels were found as 566.7 +/- 738.5 pg/ml in the diabetics with LVDD detected, 166.3 +/- 137.1 pg/ml in the diabetics without LVDD and 134.5 +/- 77.2 pg/ml in the control group. Levels of NT-proBNP were significantly higher in the group with left ventricular diastolic dysfunction (p < 0.05). However, when the levels of NT-proBNP in the diabetic patients without LVDD were compared with the controls, the difference was not significant (p > 0.05). NT-proBNP levels were found significantly higher in LVDD group compared to the controls without a difference between the ejection fractions (p < 0.05). Conclusion: High levels of NT-proBNP was correlated tissue Doppler echocardiography findings in type 2 DM patients with preserved ejection fraction. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
Changes in Plasma Amino Acid Levels in Crimean-Congo Hemorrhagic Fever Patients
(Pera Yayincilik Hizmetleri, 2024) Ertemur, Zeynep; Aydin, Huseyin; Engin, Aynur
Objective: Crimean Congo Hemorrhagic Fever (CCHF) has an important place in viral hemorrhagic fever. Plasmaaminoacid (AA)levels of patientswhowerediagnosedwith CCHF in the acute and convalescent period of the disease were investigated in this study. Methods: 35 patients were included in the study specific polymerase chain reaction (PCR) and/or IgM antibody positivity for CCHF virus. AA levels were measured in the plasma derived from the blood samples of the patient and control groups, using liquid chromatography-mass spectrometry (LC-MS/MS) technique. Results: In our study, we observed that plasma aspartate, glutamate, histidine, leucine, phenylalanine, tyrosine levels increased statistically significantly (p<0.05), while some AAlevelsdecreased(p<0.05)inacutestagepatientscomparedtothecontrolgroups.In addition,whiletherewasanincreaseinplasmaglutamatelevelsofconvalescentpatients compared to the control groups (p<0.05), there was a significant decrease in other AA levels (p<0.05). Conclusion: Further studies to investigate the relationship between increased or decreased AAs in the plasma levels of these patients and the immune system are likely to contribute to a better understanding of the pathogenesis of the disease and to guide the approach to whether AA supplementation is necessary for treatment.
Comparison of caspase-8, granzyme B and cytochrome C apoptosis biomarker levels in orthopedic trauma patients
(Turkish Assoc Trauma Emergency Surgery, 2020) Pazarci, Ozhan; Aydin, Huseyin; Kilinc, Seyran
BACKGROUND: The primary objective of this study was to investigate whether or not apoptosis is induced following bone fracture, and if so, to investigate whether the extrinsic or intrinsic pathway of cell death is stimulated. METHODS: A total of 30 patients who presented at our clinic and were diagnosed with bone fracture following trauma were included in the study group. A control group was formed of 37 age and gender-matched volunteers. On the day after the fracture, blood samples taken from the patients were examined for cytochrome C, granzyme B and caspase-8 with the ELISA method. RESULTS: A total of 67 individuals were evaluated (fracture group: 30, control group: 37) in this study. Caspase-8 was found to be statistically significantly high in the patient group (0.37 +/- 0.06 ng/mL, p=0.002). No significant difference was determined between the groups in respect to cytochrome C values (p=0. 173). The granzyme B values were determined to be significantly high in the patient group (52.56 +/- 8.51 pg/mL, p=0.007). CONCLUSION: These results obtained from patients with a long bone fracture demonstrated that serum caspase-8 and granzyme B levels were higher in patients than in the control group, thereby showing activation of the extrinsic pathway. However, no significant difference was determined between the groups concerning serum cytochrome C levels. This study may guide future studies designed for better understanding of the molecular pathways that govern the events during a fracture, which will be important for the future advancement of fracture treatment.
Comparison of sulfur transferases in various tissue and mitochondria of rats with type 1 diabetes mellitus induced by streptozotocin
(SPRINGER INDIA, 2016) Aydin, Huseyin; Celik, Veysel Kenan; Sari, Ismail; Tekin, Yusuf Kenan; Demirpence, Ozlem; Bakir, Sevtap
This study aims to investigate the relationship between sulfurtransferase (STS) activities [rhodanese (TST), mercaptopyruvate sulfurtransferase (MST)] involved in the catalysis of several biochemical reactions including detoxification of cyanide (CN-), restructuring of Fe-S cluster in proteins, and detoxification of oxygen radicals. Rats with type 1 diabetes mellitus induced by streptozotocin (STZ) were anesthetized at 14th day, and liver, lung, kidney, and heart tissues were extracted. All samples were homogenized, and mitochondrial parts were separated. Same processes were performed also in the control group, and TST and MST activities were measured in each part. The homogenate MST (MST (Homo) .) activities of the type 1 diabetes mellitus group were compared with the control group, and a decrease was observed in the lung, liver, and kidney, respectively; at the same time, an increase was seen in the heart tissue. The mitochondrial MST (MST (Mito) .) activities of rats with type 1 diabetes mellitus group were compared with the control group, and a decrease was found in all tissues. The highest decrease in the TST (Mito) . level of rats with type 1 diabetes mellitus was observed in kidney tissue. The TST activities of the type 1 diabetes mellitus group were compared with the control group, and a decrease was observed in the liver, lung, and kidney, respectively; at the same time, an increase was seen in the heart tissue. It is demonstrated in the present study that decreases occur both in enzyme levels of tissue homogenates and in mitochondria, of rats with induced type 1 diabetes mellitus. However, these results were not statistically significant. In the presence of these findings, we think that kidney, liver, lung, and heart tissue can be affected by type 1 diabetes in the long term.
Effect of Chronic Periodontitis on Serum and Gingival Crevicular Fluid Oxidant and Antioxidant Status in Patients With Familial Mediterranean Fever Before and After Periodontal Treatment
(AMER ACAD PERIODONTOLOGY, 2014) Bostanci, Vildan; Toker, Hulya; Senel, Soner; Ozdemir, Hakan; Aydin, Huseyin
Background: The aim of this study is to investigate the impact of periodontal status on oxidant/antioxidant status in patients with chronic periodontitis (CP) who experienced familial Mediterranean fever (FMF) and their response to non-surgical periodontal therapy. Methods: Data were obtained from 13 patients with FMF with generalized CP (FMF-CP), 15 systemically healthy patients with generalized CP, 15 systemically and periodontal healthy controls (HCs), and 14 periodontally healthy patients with FMF (FMF-HC). Each participant's total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) in their gingival crevicular fluid (GCF) and serum were recorded. Probing depth, clinical attachment level, and gingival and plaque indices in each participant were also measured. The GCF and clinical parameters at baseline and 6 weeks after periodontal treatment were recorded. Results: The study showed statistically significant improvement of clinical parameters in both FMF-CP and CP groups after periodontal treatment. The baseline GCF-TOS and OSI levels were significantly higher in the CP group compared with the FMF-CP group (P < 0.05). After periodontal treatment, the GCF-TOS levels were significantly reduced in members of the FMF-CP group (P < 0.05). The GCF-TAS levels in members of the FMF-CP group were significantly higher than those of members of the HC group at baseline (P < 0.05). Serum-TAS levels in the FMF-CP group were significantly higher than those in the CP and HC groups at baseline (P < 0.05). The GCF-TOS level in the FMF-CP group was significantly higher than that in the FMF-HC group at baseline and 6 weeks. However, there were no significant differences in the serum-TOS and serum-OSI levels of those in the FMF-CP and CP groups at baseline and 6 weeks (P > 0.05). Conclusion: The results of the present study show that patients with FMF-CP displayed reduced oxidative stress and increased antioxidant status compared with those in the CP and HC groups.
The effects of non-surgical periodontal therapy on oxidant and anti-oxidant status in smokers with chronic periodontitis
(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Akpinar, Aysun; Toker, Hulya; Ozdemir, Hakan; Bostanci, Vildan; Aydin, Huseyin
Aim: The aim of this study was to determine the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) and serum oxidant-antioxidant levels in smoking and non-smoking patients with chronic periodontitis. Methods: Twenty-nine patients with chronic periodontitis (15 smokers (CP-S) and 14 nonsmokers (CP-NS)) and 20 periodontally healthy subjects (10 smokers (H-S) and 10 non-smokers (H-NS)) totalling 49 subjects were included in this study. GCF was collected from at least two pre-selected sites (one moderate and one deep pocket) in patients with CP. In the healthy group, GCF samples were collected from one site. Probing pocket depth, clinical attachment level (CAL), gingival and plaque indices, and bleeding on probing were measured. To determine serum total oxidant status (TOS) and total antioxidant status (TAS), venous blood was drawn from each subject. The GCF, serum sampling, and clinical measurements were recorded at baseline and 6 weeks after periodontal treatment. Results: The study showed statistically significant improvement of clinical parameters after periodontal treatment in both smokers and non-smokers. In the CP-S group, there were no significant differences in GCF TAS levels at both moderate and deep pocket sites between baseline and 6 weeks (p > 0.05). GCF TAS levels in the CP-NS groups were significantly increased (p < 0.05) at moderate and deep pocket sites between baseline and 6 weeks. GCF TOS levels in the CP-S groups were significantly decreased (p < 0.05) at deep pocket sites between baseline and 6 weeks. There was no significant difference in serum TAS levels of the all periodontitis patient groups between at baseline and 6 weeks (p > 0.05). Serum TOS levels in the CP-S and CP-NS groups were significantly decreased (p < 0.05) after periodontal treatments. Conclusions: The periodontal treatment improves the clinical parameters in both smokers and non-smokers. These results confirm that non-surgical periodontal therapy can reduce oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.
The Effects of the Jak-Stat Signal Pathway Inhibition on Collagen Biosynthesis in Fibroblast Cell Culture
(WILEY, 2018) Sahin, Mehtap; Aydin, Huseyin; Altun, Ahmet; Derin, Mehmet Emin; Sahin, Ali
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Evaluation of association between atherogenic index of plasma and intima-media thickness of the carotid artery for subclinic atherosclerosis in patients on maintenance hemodialysis
(WILEY-BLACKWELL, 2013) Yildiz, Gursel; Duman, Alptekin; Aydin, Huseyin; Yilmaz, Abdulkerim; Hur, Ender; Magden, Kemal; Cetin, Gulay; Candan, Ferhan
Incidence of cardiovascular diseases in the patients having chronic kidney disease (CKD) is between 25% and 60%. This increased rate is proposed to be associated with accelerated atherosclerosis. Increased carotid intima-media thickness (CIMT) is a subclinical atherosclerosis marker. Small-dense low-density lipoprotein particles are a strong risk factor for atherosclerosis. It was shown that atherogenic index of plasma (AIP=log(TG/HDL-c)) is correlated with size of the lipoprotein particles. We investigated the correlation between AIP and CIMT which is a subclinical atherosclerosis marker, in hemodialysis (HD) patients. A total of 62 persons with 31 patients under HD therapy and 31 volunteers were included in the study. In all the participants, CIMT was measured and AIP were calculated. AIP and CIMT values of the participants were compared with blood pressures, lipid profiles and the other risk factors. AIP (0.39 +/- 0.32) and CIMT (0.57 +/- 0.13) were found significantly higher in the patient group than in the controls (0.04 +/- 0.36 and 0.45 +/- 0.119, respectively); (P=0.0001 and 0.0001, respectively). There was a significant correlation between AIP and increased CIMT in the patient group (P=0.0001, r=0.430). Among the lipid parameters, the strongest correlation was found between CIMT and AIP. We demonstrated the significant increase of AIP and CIMT in HD patients. A correlation was found between AIP and CIMT. AIP was found to show a correlation with a greater number of risk factors, both classical and CKD specific, than CIMT. These data suggest that AIP might be a method which can be used both in diagnosis of subclinical atherosclerosis and in deceleration processes of its progression.
EVALUATION OF ASSOCIATION BETWEEN ATHEROGENIC INDEX OF PLASMA AND INTIMA-MEDIA THICKNESS OF THE CAROTID ARTERY FOR SUBCLINIC ATHERSCLEROSIS IN PATIENTS ON MAINTENANCE HEMODIALYSIS
(OXFORD UNIV PRESS, 2013) Yildiz, Gursel; Duman, Alptekin; Aydin, Huseyin; Yilmaz, Abdulkerim; Hur, Ender; Magden, Kemal; Cetin, Gulay; Candan, Ferhan
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Evaluation of ovarian reserve using anti-mullerian hormone and antral follicle count in Sjogren's syndrome: Preliminary study
(WILEY, 2017) Karakus, Savas; Sahin, Ali; Durmaz, Yunus; Aydin, Huseyin; Yildiz, Caglar; Akkar, Ozlem; Dogan, Mansur; Cengiz, Ahmet; Cetin, Meral; Cetin, Ali
AimThe aim of this study was to determine ovarian reserve status using anti-mullerian hormone (AMH) level and antral follicle count (AFC) in patients with Sjogren's syndrome (SS). MethodsTwenty-four women with SS diagnosed according to the classification criteria proposed by the American-European Consensus Group and 25 healthy women as controls were enrolled in this study. Ovarian reserve was assessed on clinical findings, AFC, and serum AMH and reproductive hormone levels. ResultsCompared with the healthy controls, in the SS patients, the duration of menstrual cycle was significantly shorter (P = 0.043); serum AMH (P = 0.001) and AFC (P = 0.001) were significantly lower, and serum luteinizing hormone (LH) was significantly higher (P = 0.019). The right (P = 0.555) and left ovarian (P = 0.386) volumes were also lower but this did not reach statistical significance. Serum follicle-stimulating hormone (P = 0.327), estradiol (P = 0.241), and prolactin (P = 0.55) were similar between the two groups. ConclusionsOvarian reserve may be reduced in SS patients. For the assessment of ovarian reserve, serum AMH and ovarian AFC with serum LH may be useful. Further studies with long-term follow-up are required to determine the course of ovarian reserve abnormalities and best possible biomarkers of reduced ovarian reserve in SS patients.
Evaluation of Ovarian Reserve with Anti-Mullerian Hormone in Familial Mediterranean Fever
(HINDAWI LTD, 2015) Sahin, Ali; Karakus, Savag; Durmaz, Yunus; Yildiz, Caglar; Aydin, Huseyin; Cengiz, Ahmet Kivanc; Guler, Duygu
Objective. To investigate ovarian reserves in attack-free familial Mediterranean fever (AF-FMF) patients at the reproductive age by anti-Mullerian hormone (AMH), antral follicle count (AFC), ovarian volume, and hormonal parameters. Methods. Thirty-three AF-FMF patients aging 18-45 years and 34 healthy women were enrolled and FSH, LH, E2, PRL, and AMH levels were measured in the morning blood samples at 2nd-4th days of menstruation by ELISA. Concomitant pelvic ultrasonography was performed to calculate AFC and ovarian volumes. Results. In FMF patient group, median AMH levels were statistically significantly lower in the M69V mutation positive group than in the negative ones (P = 0.018). There was no statistically significant difference in median AMH levels between E148 Qmutation positive patients and the negative ones (P = 0.920). There was also no statistically significant difference in median AMH levels between M680I mutation positive patients and the negative ones (P = 0.868). No statistically significant difference was observed in median AMH levels between patients who had at least one mutation and those with no mutations (P = 0.868). We realized that there was no difference in comparisons between ovarian volumes, number of follicles, and AMH levels ovarian reserves when compared with FMF patients and healthy individuals. Conclusions. Ovarian reserves of FMF pateints were similar to those of healthy subjects according to AMH. However, AMH levels were lower in FMF patients with M694V mutation.
Expression of FGF-23 and FGFR1 is increased in uremic rat skin
(Dustri-Verlag Dr Karl Feistle, 2023) Koz, Sema Tulay; Ozkaynak, Ozge; Koz, Suleyman; Aydin, Huseyin; Goze, O. Fahrettin
Purpose: Alterations in skin structure and function are very common in uremic patients, but still there is no unifying hypothesis for uremic skin disorders. Fibroblast growth factor-23 (FGF-23) deficiency has been linked to skin disorders in non-uremic animals. We aimed to study alterations in FGF-23 and fibroblast growth factor-23 receptor 1 (FGFR1) expression in uremic rat skins. Material and methods: Wistar albino rats were divided into two groups: sham group (SG, n = 8) and uremic group (UG, n = 8). Uremia was induced by reduction of the total kidney mass in the UG. Animals were sacrificed after 14 weeks of the follow-up. Results: Serum creatinine and blood urea nitrogen levels in the UG increased significantly, compared to the SG, at the end of the experiment (0.69 & PLUSMN; 0.08 vs. 0.3 & PLUSMN; 0.04 Mann-Whitney U test (MWU), p = 0,003 and 55.2 & PLUSMN; 8.9 vs. 29.6 & PLUSMN; 6.8 MWU, p = 0.002, respectively). Serum FGF-23 level in the UG was increased non -significantly, compared to the SG (53.5 & PLUSMN; 20.9 vs. 37.2 & PLUSMN; 9.7 MWU, p = 0.072), whereas serum 1,25(OH)2D3 level was significantly lower in the UG (149.4 & PLUSMN; 33.5 vs. 213.8 & PLUSMN; 43.8 MWU, p < 0.05). Expression of FGF-23 in UG skins, assessed by western blot, was significantly higher than that in the SG (186.3 & PLUSMN; 16.8 vs. 148.9 & PLUSMN; 25.9, MWU, p < 0.01). FGFR1 expression was increased in almost all parts of the uremic skin. Receptor expression was most dense at the epidermis and hair follicles. Normal skin appendages and cells either expressed no receptor, or expressed it very weakly. Conclusion: This study shows increased FGF-23 levels and FGFR1 expression in uremic rat skins. It deserves further study to fully place this finding in the pathophysiology and clinical picture of uremic skin diseases.
Glutamine depletion in patients with Crimean-Congo hemorrhagic fever
(Wiley, 2020) Aydin, Huseyin; Engin, Aynur; Keles, Sami; Ertemur, Zeynep; Hekim, Nezih
Crimean-Congo hemorrhagic fever (CCHF) is a viral disease. There is not enough knowledge about plasma amino acid levels in CCHF. Therefore, we investigated plasma amino acid levels in patients with CCHF and the association between the levels of these amino acids and disease severity. The plasma amino acid levels (including glutamate [Glu], aspartate [Asp], glutamine [Gln], asparagine [Asn] and gamma-aminobutyric acid [GABA]) in CCHF patients and controls were measured by using liquid chromatography-mass spectrometry. Plasma levels of Gln were lower while Asp, Glu, and GABA levels were higher in patients. In fatal CCHF patients, we found the plasma level of Asn was increased whereas the plasma level of GABA was decreased. This study is the first in the literature to evaluate the plasma Gln, Glu, Asn, Asp, and GABA levels in CCHF patients. We found that the plasma Gln levels were significantly lower in CCHF patients while Asp, Glu, and GABA levels were elevated. Considering that these amino acids are important for immune cells, the plasma amino acid levels of CCHF patients may contribute to the understanding of the pathophysiology of disease and it can be important for supportive treatment of CCHF.
Glutamine-Driven Metabolic Adaptation to COVID-19 Infection
(Springer India, 2023) Aydin, Huseyin; Tekin, Yusuf Kenan; Korkmaz, Ilhan; Tekin, Gulacan; Yurtbay, Sefa; Keles, Sami; Hekim, Nezih
Background COVID-19 is known to be transmitted by direct contact, droplets or feces/orally. There are many factors which determines the clinical progression of the disease. Aminoacid disturbance in viral disease is shown in many studies. In this study we aimed to evaluate the change of aminoacid metabolism especially the aspartate, glutamine and glycine levels which have been associated with an immune defence effect in viral disease. Methods Blood samples from 35 volunteer patients with COVID-19, concretized diagnosis was made by oropharyngeal from nazofaringeal swab specimens and reverse transcriptase-polymerase chain reaction, and 35 control group were analyzed. The amino acid levels were measured with liquid chromatography-mass spectrometry technology. Two groups were compared by Kolmogorov-Smirnov analysis, Kruskal-Wallis and the Mann-Whitney U. The square test was used to evaluate the tests obtained by counting, and the error level was taken as 0.05. Results The average age of the patient and control group were 48.5 +/- 14.9 and 48.8 +/- 14.6 years respectively. The decrease in aspartate (p = 5.5 x 10(-9)) and glutamine levels (p = 9.0 x 10(-17)) were significiantly in COVID group, whereas Glycine (p = 0.243) increase was not significiant. Conclusions Metabolic pathways, are affected in rapidly dividing cells in viral diseases which are important for immun defence. We determined that aspartate, glutamine and glycine levels in Covid 19 patients were affected by the warburg effect, malate aspartate shuttle, glutaminolysis and pentose phosphate pathway. Enteral or parenteral administration of these plasma amino acid levels will correct the duration and pathophysiology of the patients' stay in hospital and intensive care.
In vitro swelling studies in simulated physiological solutions and biocompatibility of NIPAM-based hydrogels with some biochemical parameters of human sera
(TAYLOR & FRANCIS INC, 2017) Isikver, Yasemin; Saraydin, Dursun; Aydin, Huseyin
In modern medicine, commonly used biomaterials originating from metals, ceramics and polymers have shown biocompatibility with blood, tissues, cells, etc., in the human body. Polymeric biomaterials are usually understood as polymeric materials and articles made from them which are used in medicine, biotechnology biomedicine, bioengineering, pharmaceutical, veterinary, food industry, agriculture and related fields. In this in vitro study, swellings and the biocompatibility of environmentally sensitive N-isopropyl acrylamide-based (ES) hydrogels such as N-isopropyl acrylamide/acrylamide (ES/0), and N-isopropyl acrylamide/acrylamide/ carboxylic acids (ES/XAc) prepared by free radical polymerization in aqueous solutions has been investigated. Selected carboxylic acids for this study were acrylic, methacrylic, crotonic, itaconic, maleic, mesaconic and aconitic acid. The equilibrium swelling of the hydrogels are investigated in simulated physiological fluids or crystalloid solutions such as HCl-KCl buffer (pH = 1.1), universal buffer (pH = 5.5), phosphate buffer (pH = 7.4), urea, isotonic NaCl, isotonic KCl, 5% dextrose, 5% dextrose+isotonic NaCl, Ringer's lactate, human blood serum and human serum albumin solution at 37 degrees C. For the analysis of biocompatibility, ES hydrogels are incubated in 5 different human sera and their biocompatibilities with some biochemical parameters have been investigated for 24h at 37 degrees C. No significant differences in values before and after the test procedures have been found. It is therefore concluded that environmentally sensitive N-isopropyl acrylamide-based hydrogels are biocompatible for biochemical parameters of human sera.
Influence of smoking on interleukin-1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after periodontal treatment
(WILEY-BLACKWELL, 2012) Toker, Hulya; Akpinar, Aysun; Aydin, Huseyin; Poyraz, Omer
Toker H, Akpinar A, Aydin H, Poyraz O. Influence of smoking on interleukin-1beta level, oxidant status and antioxidant status in gingival crevicular fluid from chronic periodontitis patients before and after periodontal treatment. J Periodont Res 2012; 47: 572577. (C) 2012 John Wiley & Sons A/S Background and Objective: The aim of this study was to evaluate the impact of smoking on the relationship between interleukin-1 (IL-1 beta) and oxidation in patients with periodontitis and response to nonsurgical periodontal therapy. Material and Methods: Data were obtained from 30 patients with generalized chronic periodontitis (15 smokers and 15 nonsmokers) and from 10 periodontally healthy controls. IL-1 beta level, total oxidant status (TOS) and total antioxidant status (TAS) were recorded in gingival crevicular fluid. Probing depth, clinical attachment level, gingival and plaque indices and bleeding on probing were also measured. The gingival crevicular fluid and clinical parameters were recorded at baseline and 6 wk after periodontal treatment. Results: The study showed statistically significant improvement of clinical parameters in both smokers and nonsmokers after periodontal treatment. Moreover, the baseline IL-1 beta levels were significantly higher in smokers compared with nonsmokers (p < 0.05). After periodontal treatment, the IL-1 beta levels were significantly reduced in both smokers and nonsmokers (p < 0.05). There were no significant differences in TOS and TAS between periodontitis patients and healthy controls at baseline and 6 wk after periodontal treatment. The level of IL-1 beta in gingival crevicular fluid was positively correlated with TOS in both smokers and nonsmokers. Conclusions: Periodontal treatment improved the clinical parameters in both smokers and nonsmokers. The results confirm that periodontal therapy has an effect on IL-1 beta levels in gingival crevicular fluid, but not on TOS and TAS.
Leptin as an InflammatoryMarker in Dialysis Patients
(ORTADOGU AD PRES & PUBL CO, 2010) Yildiz, Gursel; Yilmaz, Abdulkerim; Nur, Naim; Aydin, Huseyin; Kayatas, Mansur
Objective: Markers of chronic inflammation, such as high-sensitive C-reactive protein (hs-CRP) or tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6, are predictive for cardiovascular mortality and morbidity in patients with chronic renal failure. Leptin stimulates angiogenesis and accelerates vascular calcification and it plays an active role in the process of atherosclerotic disease. This study investigated the relationship between leptin and inflammatory markers (hs-CRP,TNF-alpha ve IL-6) and leptin as an inflammatory marker in dialysis patients. Material and Methods: We examined the relationship of serum leptin levels with inflammatory markers in dialyzed patients. Serum CRP or TNF-alpha, IL-6 and leptin levels were determined as well as other metabolic parameters. Correlation studies and multivariate regression analysis were performed among variables. Results: Our patients showed elevated serum leptin and inflammatory markers. Serum leptin levels were positively correlated with CRP, TNF-alpha, IL-6 and body mass index (BMI). Serum CRP levels were directly related to leptin levels. Conclusion: Both hemodialysis and continuous ambulatory peritoneal dialysis patients showed high levels of chronic inflammation markers. Serum leptin levels were elevated in dialysis patients and significantly correlated with inflammatory markers.

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