Yazar "Cetinkaya, Burhan" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • Küçük Resim Yok
    Öğe
    Development of A Multiplex PCR Method for Direct Detection of Common Mastitis Pathogens in Bovine Milk Samples
    (KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2017) Kalin, Recep; Karahan, Murat; Acik, Mehmet Nuri; Tasdemir, Bulent; Cetinkaya, Burhan
    The aim of this study was to evaluate a simple and rapid DNA extraction method combined with a multiplex polymerase chain reaction (mPCR) for the identification of the major mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli and Mycoplasma bovis) from milk samples. Of the 200 California Mastitis Test (CMT) positive milk samples, 45 (22.5%), 21 (10.5%) and 11 (5.5%) were detected as positive for the presence of S. aureus, S. agalactiae and E. coli by culture, respectively. In mPCR by DNA isolation method optimised here, S. aureus, S. agalactiae and E. coli were detected in 26.5% (53/200), 12% (24/200) and 6% (12/200) of the milk samples, respectively. The abovementioned agents were observed in similar proportions when the samples were analysed by a commercial DNA isolation kit. On the other hand, M. bovis was not detected in any of the milk samples by either culture or mPCR methods. A significant difference was determined between the results of culture and mPCRs (P< 0.001). Diagnostic sensitivity and specificity of the optimised mPCR were calculated as 100% and 89.2% respectively, when culture results were considered as reference. The results suggest that the mPCR assay employed in this study could be used as an alternative routine diagnostic method for rapid, sensitive, and specific simultaneous detection of major mastitis agents in bovine milk samples.
  • Küçük Resim Yok
    Öğe
    Investigation of Virulence and Cytolethal Distending Toxin Genes in Campylobacter spp. Isolated from Sheep in Turkey
    (MARY ANN LIEBERT INC, 2013) Acik, Mehmet N.; Karahan, Murat; Ongor, Hasan; Cetinkaya, Burhan
    The presence of virulence and cytolethal distending toxin (Cdt) genes was investigated in isolates of Campylobacter jejuni, C. coli, C. lanienae, and C. lari that originated from intestinal contents and gallbladders of clinically healthy sheep. These genes have important roles in the pathogenicity of campylobacters. A total of 363 Campylobacter isolates (221 C. jejuni, 135 C. coli, five C. lanienae, and two C. lari) were used in this study. The frequency of racR, dnaJ, ciaB, pldA, flaA, and cadF virulence genes in all the isolates were determined to be 34.4%, 30%, 24.8%, 30.9%, 95%, and 81.3%, respectively, while the virB11 virulence gene could not be detected in any isolates. CdtA, cdtB, and cdtC genes were detected in 54.5%, 55.9%, and 52.3% of the isolates, respectively. None of the virulence and toxin genes examined here were detected in a total of 19 Campylobacter isolates consisting of 10 C. jejuni and nine C. coli. This is the first study investigating the presence of virulence and toxin genes in a large number of Campylobacter species isolated from clinically healthy sheep by scanning a large area. In addition, this is the first report investigating the presence of virulence and toxin genes in sheep-originated C. lanienae and C. lari isolates.
  • Küçük Resim Yok
    Öğe
    Investigation of Yersinia ruckeri Infection in Rainbow Trout (Oncorhynchus mykiss Walbaum 1792) Farms by Polymerase Chain Reaction (PCR) and Bacteriological Culture
    (KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2012) Seker, Engin; Karahan, Murat; Ispir, Unal; Cetinkaya, Burhan; Saglam, Naim; Sarieyyupoglu, Mustafa
    This study has been carried out to investigate the presence of Yersinia ruckeri, the causative agent of Enteric Red Mouth (ERM) disease, in rainbow trout fish breeding farms located in Elazig and Malatya provinces in Eastern Turkey. For this purpose, bacteriological culture and a specific polymerase chain reaction (PCR) have been compare with examine blood and intestine samples collected from adult and young rainbow trout fish as well as water samples collected from all the farms. Y. ruckeri was isolated and identified from eight of the 17 fish farms giving a proportion of 52.9% at fish farm. The disease was noted in five of the rainbow trout farms located in Elazig and three of the 11 rainbow trout farms located in Malatya. The agent was detected in five of the six young fish breeding farms. The isolation percentages of the causative agent at fish level were calculated as 25.7% (131/510) in adult and 31.7% (19/60) in young fish, giving an overall proportion of 26.3% (150/570). All the isolates have been successfully identified as Y ruckeri by species-specific PCR. No isolation could be made from water samples. It is therefore concluded that Y. ruckeri infection poses a significant threat to the fish farms in the study area and necessary precautions should urgently be taken in order to minimize economical losses due to ERM disease. A specific PCR can be utilized successfully in aquaculture for rapid identification of bacterial agents which will help to prevent spread of infectious diseases and will therefore contribute to the productivity of fishery sector.
  • Küçük Resim Yok
    Öğe
    Isolation and Molecular Characterization of Escherichia coli O157 from Broiler and Human Samples
    (Mary Ann Liebert Inc, 2012) Kalin, Recep; Ongor, Hasan; Cetinkaya, Burhan
    There is a lack of information about the role of poultry, specifically chicken, in transmission of Escherichia coli (E. coli) O157 and subsequent human illnesses. This study was therefore aimed at investigating the presence of E. coli O157 and its virulence genes in various samples collected from broiler chickens and humans in Eastern Turkey by culture, immunomagnetic separation (IMS), and polymerase chain reaction (PCR). The genetic relationship between broiler and human isolates was also examined by pulsed-field gel electrophoresis (PFGE). In the PCR analysis of sorbitol-negative isolates, E. coli O157 was identified in 0.1% (1/1000) and 0.4% (4/1000) of the liver and cecum samples of broiler chickens, respectively. On the other hand, none of the carcass samples were determined to be positive for E. coli O157. Overall, the results indicated that 12% (3/25) of the flocks were positive for E. coli O157. The differences between the flocks in terms of the positivity were determined to be statistically significant (p < 0.001). Ten (2.7%) of 367 human stool samples were also positive for E. coli O157 in the PCR examination. None of the broiler and human E. coli O157 isolates possessed H7, shigatoxins 1-2, or enterohemolysin genes, whereas all the broiler isolates and one of the human isolates were positive for intimin gene. In the PFGE analysis, a total of eight different profiles (four from broiler and four from human isolates) were observed. However, there were no genetic relationships between broiler and human E. coli O157 isolates. It can be concluded that more detailed studies are needed in poultry to better understand the role of these species in the epidemiology of E. coli 0157 infections in humans.
  • Küçük Resim Yok
    Öğe
    Production, characterization and therapeutic efficacy of egg yolk antibodies specific to Nosema ceranae
    (Public Library Science, 2024) Acik, Mehmet Nuri; Karagulle, Burcu; Yakut, Seda; Ozturk, Yasin; Kutlu, Mehmet Ali; Kalin, Recep; Cetinkaya, Burhan
    Nosema disease, caused by Nosema ceranae, one of the single-celled fungal microsporidian parasites, is one of the most important and common diseases of adult honey bees. Since fumagillin, which has been used for decades in the control of Nosema disease in honey bees (Apis mellifera), poses a toxic threat and its efficacy against N. ceranae is uncertain, there is an urgent need to develop alternative prophylactic and curative strategies for the treatment of this disease. The main aim of this study was to investigate the therapeutic potential of specific egg yolk immunoglobulins (IgY) on Nosema disease. For this purpose, the presence of N. ceranae was determined by microscopic and PCR methods in honey bees collected from Nosema suspicious colonies by conducting a field survey. Layered Ataks chickens, divided into four groups each containing 20 animals, were vaccinated with live and inactivated vaccines prepared from field isolates of N. ceranae. Eggs were collected weekly for 10 weeks following the last vaccination. IgY extraction was performed using the PEG precipitation method from egg yolks collected from each group, and the purity of the antibodies was determined by SDS-PAGE and Western Blot. The presence of N. ceranae-specific IgYs was investigated by Western Blot and indirect ELISA methods. It was determined that specific IgYs showed high therapeutic efficacy on Nosema disease in naturally infected bee colonies. In addition, honey bees collected from infected colonies were brought to the laboratory and placed in cages with 30 bees each, and the effectiveness of IgYs was investigated under controlled conditions. It was detected that specific IgY reduced the Nosema spore load and the number of infected bees significantly in both the field and experimental study groups treated for seven days. It was concluded that chicken IgYs, an innovative and eco-friendly method, had a significant potential for use as an alternative to antifungal drugs.