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Öğe Anticancer and Cytotoxic Activities of Some Trigonella Species on MCF-7, L929 and HeLa Cell Lines(Pleiades Publishing Inc, 2024) Mojarradgandoukmolla, Shahrokh; Akan, Hasan; Kasim, Takim; Dastan, Sevgi Durna; Aktas, Hatice GumushanTrigonella filipes Boiss, Trigonella strangulata Boiss., and Trigonella uninata Banks and Sol., are three commonly used species that grow well in Iraq's northern suburbs and are frequently consumed and used in traditional medicine. The current study was planned to assess and identify the active chemicals in these plant species, as well as to analyze their anticancer and antiprofeliration effects in rats treated with alcohol and cholesterol. Plant extracts were tested for oxidative stress index (OSI), total oxidant status (TOS), and total antioxidant status (TAS). Administration of Trigonella foenum-graecum and Trigonella monantha significantly improved these changes in diseased rats. MTT cytotoxicity test was used to investigate the cytotoxic properties of Trigonella filipes, Trigonella strangulata, and Trigonella uncinata plant extracts on three distinct cell lines in varied concentrations (1-10-100-1000 g/mL) after 24 h of incubation. The results were supported that these three plant taxa have high phytochemical compounds that have physiological effects such as inhibit cell proliferation, improving the function of liver and kidney function, and antioxidants effect.Öğe The effectiveness of platelet-rich plasma in an anosmia-induced mice model(WILEY, 2018) Yasak, Ahmet Gorkem; Yigit, Ozgur; Server, Ela Araz; Dastan, Sevgi Durna; Gul, MehmetObjectiveIn this study, we aimed to functionally and morphologically demonstrate the effectiveness of platelet-rich plasma (PRP) on anosmia in a mouse model of anosmia. Study DesignAnimal study. MethodsA total of 16 male mice were included. When selecting the mice, the food-finding test (FFT) was used to make sure that the animals could smell, and anosmia was induced by administration of intraperitoneal 3-methylindole. The mice were randomly divided into two groups of eight (groups A and B). After 1 week, topical PRP was administered to the mice in group A and topical saline was administered to the mice in group B. The FFT was again administered at 7, 14, and 21 days. The mice were sacrificed on day 21, the olfactory neuroepithelium was histopathologically examined, and the epithelial damage scores and epithelial thickness were measured. ResultsAfter topical administration of PRP and saline, the difference in the average FFT values of the groups was statistically significant at 7, 14, and 21 days (P < 0,005). During the histopathological examination, the epithelial damage score was statistically significantly lower in the PRP group (P=0.001) than in the saline group, and epithelial thickness was statistically significantly greater in the PRP group compared to the saline group (P=0.003). ConclusionWe showed that PRP administration has a curative effect on olfactory functions in an anosmia-induced mice model. However, there is a need for further research before PRP can be considered for use in patients with anosmia in clinical practice. Level of EvidenceNA. Laryngoscope, 128:E157-E162, 2018Öğe The effects of wireless electromagnetic fields on the activities of carbonic anhydrase and acetylcholinesterase enzymes in various tissues of rats(WILEY, 2018) Kocyigit, Umit Muhammet; Taslimi, Parham; Gurses, Fatih; Soylu, Sinan; Dastan, Sevgi Durna; Gulcin, IlhamiThe purpose of our study is to assist in understanding the effects of wireless electromagnetic waves on carbonic anhydrase (CA) and acetylcholinesterase (AChE) enzymes activities in the different tissues of the rats. For this purpose, two different groups each of which contains eight rats (n=8) were formed as being control group and wireless electromagnetic wave-administered group. The rats were necropsied after 60min from the injection of chemicals into the rats intraperitoneally. The different tissues of the rats were extracted. CA and AChE enzymes activities were measured for each tissue. All the experimental results were provided in mean +/- S.D. Statistical significance was identified to be P<0.05. It was observed that there were significant changes of enzyme activities in wireless-administered group in salivary gland, stomach, colon, liver, and striated muscle tissues.Öğe GENE EXPRESSION LEVELS IN BRONCHIAL ASTHMA PATIENTS OF DUHOK POPULATION IN IRAQ(Parlar Scientific Publications (P S P), 2021) Issa, Hamdia Yousif; Al-Habib, Omar A. M.; Dastan, Sevgi Durna; Ramadhan, Ali A.; Sevindik, Mustafa; Selamoglu, ZelihaBronchitis, asthma, allergy and bronchial asthma diseases are very common in these years. These diseases, which have increased with the age in the past, are nowadays frequently seen from very early ages and even from infancy. In addition. the frequency of these diseases varies according to different populations. It is suggested that environmental factors, genotypic structure of individuals have special effects or additive effects in these diseases which are also associated with the immune system not working effectively. The aim of our study is to determine the expression levels in some genes that are suggested to be related to these diseases in blood samples between the patient and control groups. In this study, gene expression values performed with the RT-PCR analysis are compared between brochititis-asthma patient group and control group by using 16 gene region. According to RTPCR gene expression analysis, individuals with bronchial-asthma have had remarkable increases or decreases in some gene regions. Some of these changes were found statistically significant.Öğe Genetic Diversity of Cyprinion macrostomus Heckel, 1843 (Teleostei: Cyprinidae) in Anatolia(CENTRAL FISHERIES RESEARCH INST, 2012) Dastan, Sevgi Durna; Bardakci, Fevzi; Degerli, NaciIn this study, the phylogenetic and phylogeographic relationships among Cyprinion macrostomus HECKEL, 1843, Cyprinion kais HECKEL, 1843 and Carasobarbus chantrei SAUVAGE, 1882 samples from Anatolia were analysed. While doing this, mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and Nuclear DNA inter-simple sequence repeat (ISSR) were used. PCR amplified the complete mtDNA NADH 3/4 dehydrogenase (ND-3/4) gene. Amplified fragment was digested by 14 restriction enzymes that produced 14 composite haplotypes for these populations. All identified mtDNA haplotypes differed from each other having a specific genetic profile. The genetic diversity among the mtDNA haplotypes of C. macrostomus, C. kais, and C. chantrei populations from drainage basins of the Mediterranean and those from the drainage basin of the Persian Gulf was purported by the extrapolation of mtDNA PCR-RFLP data. This result is also supported by the data explaining the geological history of Anatolia. Broadly, the data from mtDNA and from nDNA was consistent.Öğe Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential(Hindawi Ltd, 2021) Tyagi, Pankaj Kumar; Gupta, Samridhi; Tyagi, Shruti; Kumar, Manoj; Pandiselvam, R.; Dastan, Sevgi Durna; Sharifi-Rad, JavadSpinacia oleracea (spinach) and Musa acuminata (banana) were chosen for the study, and aqueous extracts of spinach leaf extract (SLE) and banana peel extract (BPE) were prepared for the synthesis of iron nanoparticles (FeNPs), and their antibacterial potential against pathogenic bacteria Bacillus subtilis (MTTC 1133) and Escherichia coli (MTTC 62) was evaluated. In 10 minutes at 60 & DEG;C, the color of the mixture (FeCl3+SLE) changed from light green to dark blackish-brown, and the color of the mix (FeCl3+BPE) changed from transparent yellow to dark black, confirming the synthesis of FeNPs from SLE and BPE, respectively. The UV-Vis spectra of spinach- and banana-derived FeNPs revealed two peaks ranging from 240 to 430 nm and multiple peaks at 240, 270, and 395 nm, respectively. FTIR spectroscopy was used to show different functional groups on BPE and SLE, and their role in FeNP synthesis was predicted. TEM micrographs showed that the particles were in nanoscale, ranging in size from 20 to 50 nm for BPE-derived FeNPs and 10 to 70 nm for SLE-derived FeNPs. The FeNP (BPE and SLE) XRD analysis revealed amorphism, with a weak iron characteristic peak, indicating noncrystallinity. The antibacterial potential of BPE- and SLE-FeNPs was investigated, and inhibition zones (mm) against B. subtilis (22.70 & PLUSMN;0.4) and E. coli (20.45 & PLUSMN;1.66) were observed, as well as SLE-FeNPs against B. subtilis (23.56 & PLUSMN;1.00) and E. coli (20.33 & PLUSMN;0.58). There were no significant differences in antibacterial activities between BPE-FeNPs and SLE-FeNPs. Positive controls were tetracycline and gentamicin, both standard antibiotics, at 5 mu g/disk. SLE- and BPE-derived green FeNPs were also analysed in vivo of D. melanogaster life history traits, i.e., fecundity, hatchability, viability, and duration of development for toxicity evaluation. SLE- and BPE-derived green FeNPs at a concentration of 10 mg/L were fed flies compared to normal diet-fed flies (control sample), and no significant differences were observed between them. The findings suggest that FeNPs have a high antibacterial potential and could be used as antibacterial agents against pathogenic bacteria while being nontoxic in nature.Öğe Hypoxia and Normoxia Preconditioned Olfactory Stem Cells Against Noise-Induced Hearing Loss(Lippincott Williams & Wilkins, 2024) Durna, Yusuf M.; Yigit, Ozgur; Edizer, Deniz T.; Dastan, Sevgi Durna; Gul, Mehmet; Ovali, ErcumentObjective:Noise-induced hearing loss is one of the leading causes of permanent hearing loss in the adult population. In this experimental study, the authors aimed to investigate the effectiveness of hypoxia and normoxia preconditioned olfactory stem cells against noise trauma.Methods:Twenty-seven female guinea pigs were enrolled. Two guinea pigs were sacrificed for harvesting olfactory tissue and 1 for examining the architecture of the normal cochlea. The remaining 24 guinea pigs were exposed to noise trauma for 1 day and then randomly divided into 3 groups: intracochlear injection of (i) normoxic olfactory stem cells, (ii) hypoxic olfactory stem cells, and (iii) physiological serum. Auditory brainstem response (ABR) measurement was performed before and 2 weeks after noise trauma and weekly for 3 weeks following intracochlear injection. Both click and 16 kHz tone-burst stimuli were used for detection of ABR.Results:No significant difference was noted between the groups before and 2 weeks after noise trauma. ABR thresholds detected after intracochlear injections were significantly higher in the control group compared with stem cell groups. However, no significant difference was detected between the stem cell groups. Fluourescence microscopy revealed better engraftment for hypoxic stem cells. Light and electron microscopy examinations were consistent with predominant degenerative findings in the control group, whereas normoxic group had more similar findings with normal cochlea compared with hypoxic group.Conclusion:Olfactory stem cells were demonstrated to have the potential to have beneficial effects on noise trauma.Öğe In Vitro Antioxidant Evaluation of Some Mannich Bases which Contain Bis-1,2,4-Triazole Derivative(CHEM SOC PAKISTAN, 2015) Parlak, Akif Evren; Karatepe, Mustafa; Koparir, Metin; Dastan, Sevgi Durna; Alayunt, Naci Omer; Ulas, Mustafa; Keser, Serhat; Celik, SaitThis study aims to examine the antioxidant effects of some Mannich bases containing bis-1, 2, 4 triazole, which are synthesized afresh. The antioxidant activities of the derivatives were measured using different methods in this study, including reducing power capacity, metal chelating activity, superoxide anion radicals scavenging activity, H2O2 scavenging activity and hydroxyl radical scavenging. As a result, derivatives had efficient antioxidant and free radical scavenging activity when compared to ascorbic acid, BHT and alpha-tocopherol as associated antioxidants.Öğe Inflammatory protein levels in asthmatic bronchitis: A study in the Duhok population, Iraq(Elsevier Science Inc, 2025) Al-Habib, Omar A. M.; Issa, Hamdia Yousif; Dastan, Taner; Dastan, Sevgi Durna; Ramadhan, Ali A.; Selamoglu, ZelihaObjectives: This study aims to determine the levels of TNF-alpha, IGF-1, IL-6, and IL-10 protein in blood samples and their potential link to bronchitis-asthma diseases in the Iraq Duhok population, highlighting the prevalence of these long-term inflammatory diseases. Methods: Sixty blood samples were used and separated into patients (n = 43) and control (n = 17) groups. Serum samples were separated for each individual. Elisa method was used in terms of 4 different proteins investigated in blood samples with the manufacturer's instruction brand kits. Results: This study evaluated TNF-alpha, IGF-1, IL-6, and IL-10 protein levels in blood samples from asthmatic bronchitis patients in Duhok. Although these levels were elevated compared to controls, the differences were not statistically significant. Conclusion: The differences thought to be related to the bronchitis-asthma diseases could not be demonstrated between the patient and control groups in Iraq Duhok population. Future research should explore larger sample sizes and stratified patient groups to identify potential biomarkers.Öğe Inhibitory effects of some drugs on carbonic anhydrase enzyme purified from Kangal Akkaraman sheep in Sivas, Turkey(WILEY, 2018) Kocyigit, Umit M.; Dastan, Sevgi Durna; Taslimi, Parham; Dastan, Taner; Gulcin, IlhamiIn this study, carbonic anhydrase (CA) enzyme was purified and characterized from blood samples of Kangal Akkaraman sheep and inhibitory properties on certain antibiotics were examined. CA purification was composed of preparation of the hemolysate and conducting the Sepharose-4B-tyrosine-sulfanilamide affinity gel chromatography in having specific activity of 11626EUmg(-1), yield of 14.40%, and 242.76-fold purification. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed to assess the enzyme purity and a single band was observed. Some antibiotics were exhibited in vitro inhibition on the CA activity. IC50 values of these inhibitors were calculated by plotting activity percentage. IC50 values of certain drugs (dexamethasone; caffeine; metamizole sodium; tetramisol; ceftiofur HCl; ivermectin; tavilin 50; penokain G; neosym; and sulfamezathine) were found as 0.38, 8.24, 285.53, 114.77, 5.33, 2.76, 27.58, 213.50, 208.28, and 36.60M, respectively. K-i values of different drugs on Kangal Akkaraman sheep blood CA activity were found in the range of 0.21 +/- 0.038-266.64 +/- 37.11 mu M.Öğe Investigation of acetylcholinesterase and mammalian DNA topoisomerases, carbonic anhydrase inhibition profiles, and cytotoxic activity of novel bis(-aminoalkyl)phosphinic acid derivatives against human breast cancer(WILEY, 2017) Dastan, Taner; Kocyigit, Umit M.; Dastan, Sevgi Durna; Kilickaya, Pakize Canturk; Taslimi, Parham; Cevik, Ozge; Koparir, Metin; Orek, Cahit; Gulcin, Ilhami; Cetin, AhmetThe aim of this study was to evaluate biologically active novel molecules having potentials to be drugs by their antitumor properties and by activities of apoptotic caspase and topoisomerase. Following syntheses of novel eight bis(-aminoalkyl)phosphinic acid derivatives (4a-h) as a result of array of reactions, compounds were evaluated by cytotoxic effects in vitro on human breast cancer (MCF-7) and normal endothelial (HUVEC) cell lines. All phosphinic acid derivatives were effective for cytotoxicity on both MCF-7 and HUVEC lines, while 4c, 4e, and 4f compounds were found significantly more effective. For the evaluation of antitumor properties of compounds in a highly sensitive method, their effects on inhibiting topoisomerases I and II were investigated. Also, some of the bis(-aminoalkyl)phosphinic acid derivatives (4a, 4e-h) showed nice inhibitory action against acetylcholinesterase and human carbonic anhydrase isoforms I and II.Öğe Investigation of anti-proliferative and antioxidative effects of some bis (alpha-amino) phosphinic acid derivatives(UNIV KARACHI, 2016) Dastan, Taner; Dastan, Sevgi Durna; Atas, Mehmet; Orek, Cahit; Koparir, Pelin; Parlak, Akif Evren; Baral, Inanc; Koparir, Metin; Cetin, AhmetAminophosphinic acids which are organophosphorus compounds widely investigated for potential production of antibacterial, antitumor and antiviral materials. In vitro antioxidant, cytotoxic and antimicrobial activities of synthesized novel compounds of 8 different bis(a-amino alkyl)phosphinic acids (4a-h) were investigated on MCF-7 breast adenocarcinoma cell and human umbilical vein endothelial cell (HUVEC) cultures. Malondialdehyde (MDA) levels were evaluated as an indication of lipid peroxidation in cell cultures for antioxidant capacities. In vitro antioxidant activities in cell cultures were determined by evaluating totals of antioxidant, oxidant, thiol levels and activities of paraoxanase, aryl esterase. It was found that 4c compound reduced MDA level significantly while 4a and 4g compounds increased MDA levels significantly compared to control. 4c compound was found most effective in reducing MDA levels by neutralizing reactive oxygen species to prevent cell damage while compounds 4c, 4f and 4h were found presenting adequate activity with other antioxidants. In vitro anti-proliferation was evaluated on MCF-7 and HUVEC cells using XTT to investigate anti-cancer potentials as therapeutics. Compounds 4c, 4e and 4f were exhibited better compared to others. Most compounds were found cytotoxic to both MCF-7 and HUVECs. Antimicrobial and antifungal activities were investigated by disc diffusion and compared to MICs of Gentamycin and Nystatin.Öğe Investigation of gene expression levels in thyroid tissues of rats treated with Wi-Fi electromagnetic wave (2.4-3 GHz Wi-Fi RF-EMF)(Elsevier, 2023) Serin, Musa; Soylu, Sinan; Dastan, Sevgi Durna; Koc, Suleyman; Kurt, AtillaIn this study, it was aimed to examine the effects of wireless waves applied to rats in the thyroid tissue by evaluating gene expression levels on 12 determined gene regions. 20 healthy 16-week-old Wistar albino female rats weighting 200-220 g were used. In the experiments, two groups, control and experimental groups, were formed, and ten female rats were used in each group. While WI-FI electromagnetic field was applied to the experimental group of these rats, nothing was applied to the control group. Many different gene regions, including beta catenin, beta-actin, GAPDH, L3B, HIF 1-Alpha, GSk-3B, TCF, WNT7a, WNT10a, WNT2, Beclin 1 and Beclin 2, ATG5 and ATG12, were investigated. ACTB and GAPDH gene primers were used as a house keeping gene. The fold change values were calculated statistically from the data obtained from the gene expression re-sults. Compared to the control group, it was observed that the experimental group had significant increases in gene expression levels of many gene regions investigated in the study (P<0.05). Among the study groups, 12 different genes such as beta catenin, beta-actin, GAPDH, L3B, HIF 1-Alpha, GSk-3B, TCF, WNT7a, WNT10a, WNT2, Beclin 1 and Beclin 2, ATG5 and ATG12, which we have determined according to the literature, are known to be related to autophagy and oxidative stress. When the expression levels were investigated, it was determined that the expression coefficients of all the genes studied in the wnt/ss catenin pathway in the experimental group of rats exposed to the Wi-Fi EM field, except for ATG5 and ATG12, were quite high, and there was significant differences between the groups.Öğe INVESTIGATION OF THE GENETIC STRUCTURES AND PHYLOGENETIC RELATIONSHIPS FOR THE SPECIES OF THE GENUS ANTHRISCUS PERS. (APIACEAE) DISTRIBUTED IN TURKEY, USING THE NON - CODING "TRN" REGIONS OF THE CHLOROPLAST GENOME(PAKISTAN BOTANICAL SOC, 2019) Sancar, Pelin Yilmaz; Civelek, Semsettin; Tekin, Mehmet; Dastan, Sevgi DurnaThe genus Anthriscus Pers. of the family Apiaceae is a small sized genus with 16 species, distributed in the world. In Turkey it is represented by 8 taxa, distributed in 4 sections. The aim of this study was to determine the genetic proximity and distances of taxa to each other and to identify interrelationships, systematic and phylogenetic relationships using the sequence analysis information of the non-coding trn region in the chloroplast genome of the Anthriscus species in Turkey. The phylogenetic tree showed that the taxa A. caucalis var. caucalis and A. tenerrima var. tenerrima (belonging to sect. Anthriscus) with A. cerefolium var. trichocarpa (belonging to sect. Cerefolium) had completed their speciations and isolation with other species in terms of speciation was provided. It can be said that A. kotschyi, the only representative of the sect. Caroides in Turkey, is isolated having completed its speciation also. The presence of the continuing gene exchange between the taxa can be mentioned, while the taxonomy of the two taxa of A. sylvestris and A. lamprocarpa, two members of the sect. Cacosciadium, cannot be determined more clearly yet. For this reason, it can be said that the A. sylvestris subsp. sylvestris and A. sylvestris subsp. nemorosa taxa, previously identified as two subspecies belonging to A. sylvestris, should be raised again to A. sylvestris and A. nemorosa taxa. In addition, an infrageneric arrangement and subsequent taxonomic regulation need to be made for the subspecies belonging to the A. lamprocarpa taxa.Öğe Isolation and molecular identification of cellulose-degrading bacteria from rumen sheep Ovis aries and evaluation of their cellulase production(Elsevier, 2024) Ben Ghalib, Khaoula; Chadli, Mohamed; Dastan, Sevgi Durna; Elmtili, NoureddineThis study focuses on the isolation and identification of cellulose-degrading bacteria from the digestive tract of sheep ( Ovis aries) and to determine the cellulase capacity of different isolates for ulterior application. The bacterial strains were screened firstly using Congo red, where cellulase activity is indicated by the appearance of a hydrolysis zone on the Carboxymethylcellulose medium (CMC), the clearance zone value ranged between 8 and 15 mm for all isolates and the hydrolytic capacity was between 1.6 to 2.5 mm. The cellulolytic strain isolates were identified using MALDI-TOF Mass Spectrometry and 16S ribosomal RNA gene sequencing. MALDI-TOF MS test indicated that all strains belonged to Bacillaceae family. The strains Isolate2, Isolate3, and Isolate8 were reliable to B. tequilensis species with a score value 2.0. Whereas, the Isolate1, Isolate4, Isolate5, and Isolate6 were reliably identified to the genus level (Score value 1.7-1.99). The molecular identification results revealed that the strains indicate a high sequence similarity with 16S rRNA gene sequences accessible in GenBank database and belong to Priestia megaterium, Lysinibacillus capsici, Bacillus tequilensis, and Bacillus paralicheniformis. The highest Carboxymethylcellulase activity (CMCase) was obtained by B. tequilensis (0.827 +/- 0.035 U/ml) at a pH of 7.0 and temperature of 30 degrees C at 100 g. The carbon source utilized was CMC (1 %), while peptone (1 %) and ammonium sulfate (0.24 %) served as the nitrogen sources. Further research in optimizing and purifying of cellulase, could be useful for the future hydrolyzation of some green biomass for various biotechnological applications such as biofuel production.Öğe Methylation of the e-cadherin (ECAD) gene in clear cell renal cell carcinoma (CCRCC)(Parlar Scientific Publications, 2020) Kitapci, Aytul; Dastan, Taner; Dundar, Gokce; Pektas, Ayse Nur; Dastan, Sevgi Durna; Korgali, Esat; Turkoglu, SifaClear cell renal carcinoma is one of the most common sub-types of renal cell cancer. Methylation is the leading type of epigenetic changes. Many studies in the field emphasized that hypermethylation of some genes is efficient on the formation of many cancer types. In this study, the aim is to analyze DNA methylation, one of the epigenetic changes in the ECAD gene of renal cell carcinoma (RCC) patients, and to determine the relations by comparing the present epigenetic features with clinic-pathologic parameters. Studies were organized in two groups: a control group with 153 individuals and a patient group with 113 individuals. The methylation of blood samples was determined by displaying in the gel after methylation specific PCR (MSP) steps. Some descriptive statistics and Wilcoxon, Correlation and Logistic Regression analyses were performed using SPSS. As a result of this study, it was found out that the probability of the occurrence of this disease was 5 times higher in people who have methylation in their ECAD genes than those who have not. Furthermore, it was observed that women are 4.253 times more likely to have disease compared to men. This research is a very important and fundamental study on the diagnosis and treatment of CCRCC disease in terms of the effect of epigenetic mechanisms such as methylation analysis. © 2020 Parlar Scientific Publications. All rights reserved.Öğe METHYLATION OF THE E-CADHERIN (ECAD) GENE IN CLEAR CELL RENAL CELL CARCINOMA (CCRCC)(Parlar Scientific Publications (P S P), 2020) Kitapci, Aytul; Dastan, Taner; Dundar, Gokce; Pektas, Ayse Nur; Dastan, Sevgi Durna; Korgali, Esat; Turkoglu, SifaClear cell renal carcinoma is one of the most common sub-types of renal cell cancer. Methylation is the leading type of epigenetic changes. Many studies in the field emphasized that hypermethylation of some genes is efficient on the formation of many cancer types. In this study, the aim is to analyze DNA methylation, one of the epigenetic changes in the ECAD gene of renal cell carcinoma (RCC) patients, and to determine the relations by comparing the present epigenetic features with clinic-pathologic parameters. Studies were organized in two groups: a control group with 153 individuals and a patient group with 113 individuals. The methylation of blood samples was determined by displaying in the gel after methylation specific PCR (MSP) steps. Some descriptive statistics and Wilcoxon, Correlation and Logistic Regression analyses were performed using SPSS. As a result of this study, it was found out that the probability of the occurrence of this disease was 5 times higher in people who have methylation in their ECAD genes than those who have not. Furthermore, it was observed that women are 4.253 times more likely to have disease compared to men. This research is a very important and fundamental study on the diagnosis and treatment of CCRCC disease in terms of the effect of epigenetic mechanisms such as methylation analysis.Öğe Molecular diversity of Klebsiella pneumoniae clinical isolates: antimicrobial resistance, virulence, and biofilm formation(Taylor & Francis Inc, 2024) Kafa, Ayse Humeyra Taskin; Aslan, Rukiye; Dastan, Sevgi Durna; Celik, Cem; Hasbek, Mursit; Eminoglu, AysenurOne of the mechanisms responsible for antibiotic resistance in Klebsiella pneumoniae is the enzymes produced by the bacteria; another important mechanism is the ability to form biofilm. In this study, antibiotic resistance, genes associated with virulence, and biofilm-forming properties of K. pneumoniae strains were investigated. A total of 100 K. pneumoniae isolates were obtained from different clinical samples identified by Matrix-Assisted Laser Desorption/Ionization time-of-flight Mass Spectrometry. Antimicrobial susceptibility testing was performed with the Phoenix 100 apparatus. The biofilm forming properties of strains were determined by the microtiter plate method. For molecular analysis, genes encoding the carbapenemase enzyme (bla(OXA-48), bla(NDM-1), bla(IMP), and bla(VIM)) and biofilm-related genes (treC, luxS, mrkA, and wza) were investigated by polymerase chain reaction (PCR). While 76% of clinical isolates were resistant to three or more antimicrobials, 24% were classified as non-multidrug resistant (non-MDR). When biofilm-forming capacities of clinical isolates were tested, it was determined that the resistant-isolates produced 59.2% strong biofilm, and susceptible-isolates produced 12.5% strong biofilm. According to PCR results, carbapenemase genes were determined as follows: bla(OXA-48)-70%, bla(NDM)-49%, and bla(KPC)-19%, bla(OXA-48)/bla(NDM)/bla(KPC)-12%, bla(OXA-48)/bla(NDM)-26%, and bla(OXA-48)/bla(KPC)-4%. The biofilm-associated genes in bacterial isolates were determined as follows: luxS-98%, treC-94%, mrkA-88%, and wza-15%. In addition, Hierarchical Clustering Tree and Heatmap analysis revealed an association between isolates that lacks resistance genes and isolates lacks biofilm-formation related genes that were included in MDR or non-MDR classes. As a result, biofilm should be considered in the treatment of MDR infections, and therapy should be planned accordingly. In addition, pursuing the data and genes of antibiotic resistance is significant for combating resistance.Öğe Novel 1,2,4-triazole-maleamic acid derivatives: synthesis and evaluation as anticancer agents with carbonic anhydrase inhibitory activity(Elsevier, 2024) Tapera, Michael; Kekecmuhammed, Huseyin; Tuzun, Burak; Dastan, Sevgi Durna; Celik, Muhammed Safa; Taslimi, Parham; Dastan, TanerHybrid compounds with 1,2,4-triazole ring and a hydrazone moiety were designed and synthesized by amine acylation of uncommon triaminogunidine derivatives using maleic anhydride. Synthesized compounds were characterized by various spectral techniques, including FTIR, 1H NMR, 13C NMR, and HRMS. Furthermore, the proposed structure of TM-2 was resolved by single-crystal X-ray analysis. The compounds were evaluated for their anticancer activity in vitro against the colon cancer cell line HCT116 and the ovarian cancer cell line A2780. Among them, some compounds, TM-3, TM-4, and TM-14, exhibited remarkable antiproliferative activity in the cell line A2780, with IC50 values of 6.11, 5.15, and 5.93 mu M, respectively. Further investigation revealed that these compounds could inhibit cancerous cell growth by inducing an increase in caspase-3 activity. In addition, antioxidant capabilities, interaction with plasmid pBR322 DNA, and inhibitory effects against two physiologically and pharmacologically relevant human carbonic anhydrase (hCA) isoforms, namely hCA I and hCA II, of the synthesized compounds were also assessed. Finally, computational studies, such as DFT calculation, molecular docking, and in silico predictions of ADME/T analysis, were conducted to gain an understanding of the specific reactive sites of the compounds, the binding modes in the active sites, and the pharmacokinetic parameters of the newly synthesized hybrid compounds, which were calculated to establish their drug-likeness properties.Öğe Phenylthiocarbamide taste perception as a possible genetic association marker for nutritional habits and obesity tendency of people(UNIV KARACHI, 2015) Dastan, Sevgi Durna; Degerli, Naci; Dastan, Taner; Yildiz, Fazilet; Yildir, Yavuz; Durna, Yusuf Muhammed; Atessahin, Dilek; Karan, TunayAbility to taste Phenylthiocarbamide (PTC) a bitter molecule, is usually used to know the heritable characteristic in both genetic and physiological studies. So far, no research has yet attested whether PTC blindness relation with obesity and some nutrition behaviors of human. This stu.dy is the first attempt on a large scale to examine PTC sensitivity in healthy and overweight people in Turkish population to define in the perception of bitter senses which is associated with nutrition habits, body mass index, age, gender, and to be in stable weight. PTC taste perception was measured by tasting PTC solution filtered in a paper. The results showed that tasters were significantly more frequent (81,8%) than nontasters (18,2%) in all population. A "higher proportion of nontasters were observed in the quite fat individual group (BMI >= 40kg/m(2)). Alterations explained these differences in basic taste sensitivity, age, gender, BMI, individuals' family obesity situations, vegetarian nourishment. Increased frequency of nontasters allele is evident with obesity condition. This could be due to lack of preference for nutrition among nontasters. So the phenotypic variation in PTC sensitivity is genetic in origin; it may represent an association with obesity, dietary habits, regular weight, gender, and age.
