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Öğe Clarification on a published paper in iran j parasitol(Tehran University of Medical Sciences (TUMS), 2015) Degerli, Serpil; Tepe, Bektas[No abstract available]Öğe Clarification on a Published Paper in Iran J Parasitol(IRANIAN SCIENTIFIC SOCIETY MEDICAL ENTOMOLOGY, 2015) Degerli, Serpil; Tepe, Bektas…Öğe Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica(ELSEVIER SCIENCE BV, 2011) Tepe, Bektas; Degerli, Serpil; Arslan, Serdal; Malatyali, Erdogan; Sarikurkcu, CengizThis work deals with the evaluation of in vitro antioxidant, DNA preventing and antiamoebic activities of the water extracts of Teucrium polium and Stachys iberica. In all systems, T. polium extract exhibited excellent activity potential than that of S. iberica. The amount of total phenolics and flavonoids were high in this extract. In the presence of 40 mg/ml T. polium extract, a significant protection was observed for native super coiled DNA of pBR322 plasmid DNA. In the presence of 32 mg/ml of T polium extract, no trophozoite was after the 24th hour. (C) 2010 Elsevier B.V. All rights reserved.Öğe Effects of Enterobiasis on primary school children(ACADEMIC JOURNALS, 2010) Celiksoz, Ali; Acioz, Mehmet; Degerli, Serpil; Oztop, A. Yasemin; Alim, AhmetThe aim of the present study was to investigate the possible adverse effects of Enterobiasis vermicularis infection on primary school children in Sivas, Turkey, to know whether it affects their school success, mean weight and height or not. Seven primary schools in the central region of Sivas were included in the present study. A total number of 3659 questionnaires were completed by class teachers and parents for each student. In the questionnaire, the height and weight of children, school success and other information about children were asked. The stool and cellophane tape specimens collected from the participated students were examined by light microscopy for the diagnosis of enterobiasis. The 365 (17.0%) out of 3569 of parasitic infections were E. vermicularis along with other parasites, and the 225 (10.5%) out of 3569 had only E. vermicularis. The weights and heights were found to be lower in children with enterobiasis. When school successes of the students were compared, there were statistically significant differences between the non parasitic group and the group with E. vermicularis. Enterobiasis is still an important problem seen among primary school children causing adverse effects on school success and physical growth especially in children from the first grades of primary schools.Öğe Genotyping by Sequencing of Acanthamoeba and Naegleria Isolates from the Thermal Pool Distributed Throughout Turkey(Springer Int Publ Ag, 2020) Degerli, Serpil; Degerli, Naci; Camur, Derya; Dogan, Ozgul; Ilter, HuseyinPurpose The main goal of this study was genotyping of free-living parasites and sub-grouping of pathogenic or non-pathogenic amebae obtained from Turkey's thermal springs. In so doing, distribution and abundance of possible pathogenic or causative strain for humans, which are caused by Acanthamoeba and Naegleria strains, would be elaborated. The number of extensive studies on the general occurrence and distribution of parasitic strains is very high worldwide, but there has been a paucity of information with regard to Turkey. Methods From a total of 434 obtained thermal pool samples, free-living amebas were isolated from 148 water samples using the non-nutrient agar (NNA) culture method. Subsequently, the cultivated samples were used for DNA isolation; then 102 obtained DNA samples were subjected to PCR amplification using various primers for samples of genera Acanthamoeba and Naegleria. Ultimately, estimation of genotype or subtype was evaluated by sequencing. Results About 29 samples that belong to Acanthamoeba and Naegleria were estimated from a total of 102 amplified PCR samples. These eukaryotic PCR products which have Acanthamoeba genus appearance, generated 26 subtypes and 3 Naegleria samples. Among the 26 Acanthamoeba genotypes, 22 aligned sequences were matched with various GenBank reference samples, while the 4 divergent genotypes were not elaborated and marked as ND. Most of the Acanthamoeba genera were determined as likely dominating groups and clustered as T form within totally eight groups. Eight, seven and three subtypes were found as T4A, T15 and T11 genotypes, respectively while the remainings were ultimately found in four groups. Results confirming the predominance of T4A, which is known the most causative form, the presence in the pools. Despite being uncommon, N. fowleri, lovaniensis and australiensis were also observed among the surveyed pools. Conclusion The present study is descriptive and is not unique. However, this is the most comprehensive study of the molecular distribution sampling of thermophilic Acanthamoeba and Naegleria that confirmed and demonstrated their ubiquitous presence throughout Turkey. By this estimation, in some spas, the most and likely causative form Acanthamoeba including T4 and Naegleria fowleri has also been confirmed.Öğe Genotyping of Giardia intestinalis isolated from people living in Sivas, Turkey(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2012) Degerli, Serpil; Degerli, Naci; Celiksoz, Ali; Ozcelik, SemraAim: The technique of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) genotyping was used to characterise morphologically identical isolates of Giardia intestinalis from human stool samples. Materials and methods: In this study a total of 17 trophozoite samples, obtained either directly from stool samples or after excystation, or by duodenal aspiration, were used. A set of primers was chosen to amplify the different regions of triose phosphate isomerase (tpi) and a segment of the glutamate dehydrogenase (gdh) genes. A single-stranded conformational polymorphism technique was also used in an attempt to discriminate among some subgroups. Results: Only primers of the 683-bp segment of the tpi gene from the trophozoite samples were suitable for obtaining a PCR product. In the total of 17 trophozoite DNAs where the tpi gene segment was amplified, 9 belonged to assemblage A (53%) and 4 to assemblage B (23.5%). It was not possible to identify assemblages for the remaining 4 samples (23.5%). Conclusion: PCR RFLP tpi gene application was able to discriminate between G. intestinalis assemblage A and B, but not the other subgroups. Since assemblage A is the more prevalent subgroup compared with assemblage B, this subgroup can be said to be responsible for common Giardia infections in Turkey.Öğe In vitro amoebicidal activities of Teucrium polium and T. chamaedrys on Acanthamoeba castellanii trophozoites and cysts(SPRINGER, 2012) Tepe, Bektas; Malatyali, Erdogan; Degerli, Serpil; Berk, SeydaAmoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Teucrium polium and Teucrium chamaedrys. In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellani trophozoites and cysts were decreased during the experimental process. Both extracts showed time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, T. chamaedrys showed the strongest amoebicidal effect on the trophozoites. In the presence of 16 mg/ml or above extract concentrations, no viable trophozoites were observed within 48 h. In the case of T. polium, no viable trophozoites were observed within 48 h at 32 mg/ml concentration. As expected, cysts were found more resistant to the extracts than the trophozoites.Öğe In vitro amoebicidal activity of four Peucedanum species on Acanthamoeba castellanii cysts and trophozoites(SPRINGER, 2012) Malatyali, Erdogan; Tepe, Bektas; Degerli, Serpil; Berk, Seyda; Akpulat, H. AskinAmoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which is less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Peucedanum caucasicum, Peucedanum palimbioides, Peucedanum chryseum, and Peucedanum longibracteolatum, which are endemic in Turkish flora except P. caucasicum. Extracts were evaluated for their amoebicidal activities using an inverted light microscope. In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellani trophozoites and cysts were determined during the experimental process (72nd hour). All of the extracts showed a time and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, P. longibracteolatum showed the strongest amoebicidal effect on the trophozoites and cysts. In the case of 32 mg/ml concentration of extract, no viable trophozoites or cysts were determined between 24th and 72nd hour. Similar results were obtained from the extract at 16.0 mg/ml concentration against trophozoites. At this concentration value, number of viable cysts was determined as 10.6 +/- 2.1 in the 24th hour. trophozoites were determined in the 48th hour. At the same concentration, 51% of the cysts were killed by the extract in the 72nd hour. As expected, cysts were found more resistant to the extracts than the trophozoites.Öğe In vitro amoebicidal activity of Origanum syriacum and Origanum laevigatum on Acanthamoeba castellanii cysts and trophozoites(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012) Degerli, Serpil; Tepe, Bektas; Celiksoz, Ali; Berk, Seyda; Malatyali, ErdoganIn some patients, complete treatment of amoebic keratitis is difficult because of the resistance of cysts to therapeutic agents. The aim of this study was to evaluate the in vitro amoebicidal activity of methanolic extracts of Origanum syriacum and Origanum laevigatum. In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellanii trophozoites and cysts were decreased. Both extracts showed a time and dose dependent amoebicidal action on the trophozoites and cysts. Of the extracts tested, O. syriacum showed the stronger amoebicidal effect on the trophozoites and cysts. In the presence of 32 mg/ml extract, no viable trophozoites were observed within third hour. The extract was also found effective against the cysts within 24th hour. In the case of O. laevigatum, no viable trophozoites were observed within 72nd hour at the concentrations of 16 and 32 mg/ml. As expected, cysts were found more resistant to the extracts than the trophozoites. (C) 2012 Elsevier Inc. All rights reserved.Öğe The Investigation of Cryptosporidium spp. Prevalence in Cattle and Farmers by ELISA(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2012) Ozcelik, Semra; Poyraz, Omer; Kalkan, Kadir; Malatyali, Erdogan; Degerli, SerpilCryptosporidium spp. is an important cause of enteritis for animals and humans. As the parasite has zoonotic potential, it is aimed to investigate the prevalence in people who had close contact with livestock, in control group who lived in Centrum and cattle. Fecal samples collected from 50 farmers who lived in different villages of Sivas and 65 people to be control group lived in Sivas Center without gastrointestinal discomfort and 200 cattle and calf breeding different county during the period from September 2009 to September 2010. Fecal samples were stored in 10% formalin until examination and then studied with Cryptosporidium Stool Antigen Microwell ELISA Kit. As a result, positive rate were found 4(6.2%) in control group and 9(18%) in study group, (chi(2)=3.95, P<0.05). On the other hand, 15 (7.5%) of the cattle and calve samples gave positive reaction with Cryptosporidium spp. ELISA.Öğe Investigation of Demodex Spp. prevalence among managers and workers of health hazard bearing and sanitary establishment(ELSEVIER TAIWAN, 2012) Ozer, Ali; Karaman, Ulku; Degerli, Serpil; Colak, Cemil; Karadan, Mesut; Karci, ErdalBackground/Purpose: Two Demodex species are known to live on people. Demodex folliculorum lives in the openings of hair follicles alone or in groups. D brevis lives in the depths of the sebaceous glands alone. There are different related on the epidemiology of Demodex species have been published. Method: In this study, taking into account that the parasite is transmitted through close contact from person to person, we aimed to evaluate the relation between the presence of Demodex spp with gender and age among the Health hazard bearing and sanitary establishment operators and workers who came for porter examination. Results: For this purpose, 862 male and 215 female patients with a total of 1077 volunteers from the face area of the standard superficial skin biopsy (SSSB) method and studied samples were taken. More than five mites in 1 cm(2) density was defined as positive. In investigated samples 37.3% Demodex spp. positivity was detected. Only two were found to be D brevis and the others were D folliculorum. The study revealed statistically significant relationships between the positivity of parasites with the occupational group, age and sex. Conclusion: Since the prevalence of Demodex among healthy individuals without any complaint was found to be 37%, we believe Demodex spp should be investigated in porter examinations of people who have dermatological complaints. Copyright (C) 2012, Elsevier Taiwan LLC & Formosan Medical Association. All rights reserved.Öğe Phenolic Acid Composition and Anti-Parasitic Effects of Four Peucedanum Species on Entamoeba histolytica Trophozoites(IRANIAN SCIENTIFIC SOCIETY MEDICAL ENTOMOLOGY, 2015) Degerli, Serpil; Tepe, BektasBackground: The aim of this study was to determine phenolic acid composition and anti-parasitic effects of Peucedanum caucasicum, P. palimbioides, P. longibracteolatum and P. chryseum on Entamoeba histolytica. Methods: Methanol extracts of the plant species were prepared by soxhlet extraction. Phenolic acid compositions were determined by HPLC. Anti-proliferative effect of extracts on trophozoites was determined by using trypan blue dye exclusion test. For counting the cells, approximately a hundred of E. histolytica trophozoites were examined in each time. The data were presented as mean values with standard deviations and analyzed by repeated measures of ANOVA followed by Tukey test for post-hoc pairwise comparisons. The P-value was set at 0.05 for significance level. Results: All of the extracts showed a time and dose dependent amoebicidal action on trophozoites. Among the extracts tested, P. longibracteolatum showed the strongest amoebicidal effect on the trophozoites. As expected, this plant species also exhibited time and dose dependent activity on the trophozoites. At 4.0 mg/ml extract concentration, all of the trophozoites were killed by the extract in 72(nd) hour. Gallic (11.144 mg/g), P-hydroxybenzoic (17.646 mg/g), and o-coumaric acids (14.442 mg/g) were determined as the major phenolic acids of P. longibracteolatum. Gallic and P-hydroxybenzoic acids found in P. longibracteolatum could not be determined in other extracts. Therefore, high activity potential of this plant could probably be attributed to the presence of these phytochemicals. Conclusion: P. longibracteolatum can be further evaluated as potential therapeutic drugs for the treatment of Entamoeba infections.Öğe Photocatalytic disinfection of Giardia intestinalis and Acanthamoeba castellani cysts in water(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2008) Soekmen, Munevver; Degerli, Serpil; Aslan, AlperIn this study, disinfection of water containing Giardia intestinalis and Acanthamoeba castellani cysts with TiO2 and modified catalyst silver loaded TiO2 (Ag-TiO2) was investigated. Destruction of the parasites was evaluated after UV illumination of the suspension consisting 5 x 10(8)-13.5 x 10(8) cysts/mL in the presence of 2 g/L neat or modified TiO2 at neutral pH. In the initial stage, the solid photocatalyst particles penetrated the cyst wall and then oxidant species produced by TiO2/UV destroyed both cell wall and intracellular structure. In the case of G. intestinalis inactivation (disinfection) performance of TiO2/UV system reached 52.5% only after 25 min illumination and total parasite disinfection was achieved after 30 min illumination. However, silver loaded TiO2 seemed to be more effective as this loading provided better catalytic action as well as additional antimicrobial properties. Cell viability tests showed that parasite cysts, their walls in particular, were irreversibly damaged and cysts did not re-grow. Nevertheless the studied system seemed to be ineffective for the inactivation of A. castellani. Inactivation percentages of TiO2/UV and Ag-TiO2/UV systems were far lower than that of UV alone, being 50.1% and 46.1%, respectively. (C) 2008 Elsevier Inc. All rights reserved.Öğe The Prevalence of Pediculus humanus capitis and the Coexistence of Intestinal Parasites in Young Children in Boarding Schools in Sivas, Turkey(WILEY-BLACKWELL, 2012) Degerli, Serpil; Malatyali, Erdogan; Celiksoz, Ali; Ozcelik, Semra; Mumcuoglu, Kosta Y.The aim of this study was to investigate the prevalence of Pediculus humanus capitis and the coexistence of intestinal parasites in boarding primary schools in Sivas, Turkey. Seven hundred seventy-two students (350 [45.3%] girls, 422 [54.7%] boys) were evaluated with combing for the presence of head lice, collection of fecal samples, and examination of the perianal region for intestinal parasites using the cellophane tape method. The overall infestation rate for head lice was 6% (n = 46). Nine children had evidence of nits only (1.2%), whereas living lice and nits or eggs were found in 37 children (4.8%). Girls were significantly more commonly infested (12.9%) than boys (0.2%). Of the parameters evaluated, socioeconomic level, number of rooms per family, and size and weight of the children were statistically significantly different between the children with and without lice. Although the infestation rate of children with intestinal parasites was higher in the head louse-infested group (23.9%) than in the group of children without lice (17.6%), the differences were not statistically significant.Öğe Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch & CAMey.) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites(SPRINGER, 2012) Degerli, Serpil; Berk, Seyda; Malatyali, Erdogan; Tepe, BektasThe aim of this study is to evaluate the in vitro amoebicidal activity of the aqueous extracts of Pastinaca armenea and Inula oculus-christi from Turkey. In the presence of aqueous extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellanii trophozoites and cysts were decreased during the experimental process. Both extracts showed a time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, I. oculus showed the strongest amoebicidal effect on the trophozoites and cysts. In the presence of 32.00 mg/ml extract solution in the media, no viable trophozoites were determined from the time of 24 h. Moreover, in the presence of 16.00 mg/ml I. oculus-christi extract, no viable trophozoites were detected in 72 h of the experiment. Effectiveness of I. oculus-christi extract was found moderate against the cysts. In the presence of 32.00 mg/ml extract, only 25.3% of the total cysts were killed effectively. In the case of P. armenea, 40.3% and 23.0% of the trophozoites and cysts were killed in the presence of 32.00 mg/ml extract at the end of the experimental process (72 h), respectively.Öğe Search of house mite's fauna and investigation of relationship between house dust mite and allergy in the province of Hatay, Turkey(Kuwait Medical Assoc, 2019) Gulkan, Burcu; Degerli, Serpil; Culha, Gulnaz; Savas, Nazan; Ozcelik, SemraObjective: This study aims to search house mite's fauna and to investigate the relationship between house dust mite prevalence and house-related risk factors and allergic diseases. Design: Cross sectional study Setting : Two hundred houses in 25 different neigborhoods in Hatay province of Turkey. Hatay province is located in East Mediterranean Region with a population of 1,519,836. It has the border with Syria. The sample was selected by clustered sociodemographic region systematic random sampling method. Subjects: Two hundred samples of house dust Intervention: A survey was used. Pearson Chi-Square test was used. Main outcome measure(s): Samples of house dust in total were analyzed according to Spieksma-Boezman method of lactic acid precipitation; and the identification of the samples in which mites were detected was made according to the Coloff's type identification key. Results: Out of 200 samples of house dust, mite was detected in 57.5% of them. The most frequently found type of mite was determined as D. pteronyssinus (72.2%). There was no significant relationship found between the mite prevalence and other criteria, except the number of rooms when the ground clearance of houses, the case of garden, carpet type, washing temperature and duration of duvets, times of the insolation of pillows and quilts, heating devices, cleaning times, insolation conditions, presence of a pet and the economic situation of the family were analyzed (p > 0.05). Conclusion It is determined that mite prevalence in the province of Hatay, Turkey is higher than many regions.Öğe Search of house mite’s fauna and investigation of relationship between house dust mite and allergy in the province of Hatay, Turkey(Kuwait Medical Association, 2019) Gulkan, Burcu; Degerli, Serpil; Culha, Gulnaz; Savas, Nazan; Ozcelik, SemraObjective: This study aims to search house mite’s fauna and to investigate the relationship between house dust mite prevalence and house-related risk factors and allergic diseases. Design: Cross sectional study Setting: Two hundred houses in 25 different neigborhoods in Hatay province of Turkey. Hatay province is located in East Mediterranean Region with a population of 1,519,836. It has the border with Syria. The sample was selected by clustered sociodemographic region systematic random sampling method. Subjects: Two hundred samples of house dust Intervention: A survey was used. Pearson Chi-Square test was used. Main outcome measure(s): Samples of house dust in total were analyzed according to Spieksma-Boezman method of lactic acid precipitation; and the identification of the samples in which mites were detected was made according to the Coloff’s type identification key. Results: Out of 200 samples of house dust, mite was detected in 57.5% of them. The most frequently found type of mite was determined as D. pteronyssinus (72.2%). There was no significant relationship found between the mite prevalence and other criteria, except the number of rooms when the ground clearance of houses, the case of garden, carpet type, washing temperature and duration of duvets, times of the insolation of pillows and quilts, heating devices, cleaning times, insolation conditions, presence of a pet and the economic situation of the family were analyzed (p >0.05). Conclusion It is determined that mite prevalence in the province of Hatay, Turkey is higher than many regions. © 2019, Kuwait Medical Association. 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