Yazar "Dogan, Kubra" seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Effects of electronic cigarettes on oxidative stress markers in the rat kidney tissues(Kare Publishing, 2022) Dogan, Kubra; Saygin, Huseyin; Yalman, YucelObjectives: Electronic cigarettes (e-cigarettes) are an alternative to traditional cigarettes. Although numerous studies have been conducted regarding the effects of traditional cigarettes on oxidative stress biomarkers in the kidney, there are only a few studies on the effects of e-cigarettes. Methods: A total of 24 male Wistar albino rats were separated into three groups: Group 1 was treated with traditional cigarettes, Group 2 with e-cigarettes, and Group 3 formed the control group. Kidney homogenates and plasma samples were obtained, and the glutathione peroxidase, protein carbonyl, superoxide dismutase (SOD), catalase (CAT), lipid hydroperoxide (LPO), and symmetric dimethylarginine (SDMA) levels were examined. Results: Higher plasma SDMA levels were determined in Group 1 and Group 2 compared with Group 3 (<0.0001). Higher SOD activity was found in Group 1 compared with Group 2 (p=0.0094). Lower CAT activity was found in Group 1 compared with both Group 2 (p=0.0035) and Group 3 (p<0.0001). Higher LPO levels were determined in the traditional cigarette smoking group compared with the control group (p=0.028), and no statistically significant difference was found between the e-cigarette and the control groups. Conclusion: E-cigarettes and traditional cigarettes are associated with the dysregulation of particular oxidative stress markers in the kidney. However, e-cigarettes have less effect on some oxidative stress markers than traditional ciga-rettes. Long-term use of traditional cigarettes and e-cigarettes causes oxidative stress, which may lead to renal tissue damage and diminished kidney function. © 2022, Kare Publishing. All rights reserved.Öğe Elevated Levels of Activin-A, TNF-Alpha and IL-6 in Acromegaly(Maik Nauka/Interperiodica/Springer, 2021) Dogan, Kubra; Yildiz, Seyma Nur; Sariakcali, Baris; Duman, Gulhan; Bolat, SerkanAlthough systemic inflammation has been linked to acromegaly, little is known about the abnormalities in cytokine networks during the disease. The aim of this study was to evaluate the serum concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6), and activin-A in acromegaly, and to investigate the relationship between TNF-alpha, IL-6, activin-A and comorbidities of acromegaly. A total of 56 subjects were recruited to the study. Serum samples were collected from 31 acromegaly patients and 25 healthy control subjects. Serum activin-A, IL-6 and TNF-alpha were determined using the enzyme-linked immunosorbent assay. Circulating levels of activin-A, TNF-alpha, and IL-6 were higher in patients than in the control group. Receiver operating characteristic analysis for activin-A for the differentiation of acromegaly patients from healthy control subjects showed an area under the curve of 0.90. Higher activin-A levels were determined in patients with residual pituitary tumor. These findings provide evidence for the role of activin-A, TNF-alpha and IL-6 in acromegaly. Serum activin-A levels could be used as a novel biomarker to diagnose acromegaly. These findings confirm previous studies that inflammation might be involved in the pathogenesis of acromegaly. Finally, treatment strategies targeting the regulation of inflammation cannot be sufficient alone in reducing comorbidities.Öğe Exploring free amino acid profiles in CCHF patients: Implications for disease progression(Wiley, 2024) Buyuktuna, Seyit Ali; Yerlitas, Serra Ilayda; Zararsiz, Gozde Ertuk; Dogan, Kubra; Kablan, Demet; Bagci, Gokhan; Ozer, SeldaThis study investigated the intricate interplay between Crimean-Congo hemorrhagic fever virus (CCHFV) infection and alterations in amino acid metabolism. Our primary aim is to elucidate the impact of Crimean-Congo hemorrhagic fever (CCHF) on specific amino acid concentrations and identify potential metabolic markers associated with viral infection. One hundred ninety individuals participated in this study, comprising 115 CCHF patients, 30 CCHF negative patients, and 45 healthy controls. Liquid chromatography-tandem mass spectrometry techniques were employed to quantify amino acid concentrations. The amino acid metabolic profiles in CCHF patients exhibit substantial distinctions from those in the control group. Patients highlight distinct metabolic reprogramming, notably characterized by arginine, histidine, taurine, glutamic acid, and glutamine metabolism shifts. These changes have been associated with the underlying molecular mechanisms of the disease. Exploring novel therapeutic and diagnostic strategies addressing specific amino acids may offer potential means to mitigate the severity of the disease.Öğe Exploring free amino acid profiles in Crimean-Congo hemorrhagic fever patients: Implications for disease progression(Wiley, 2024) Buyuktuna, Seyit Ali; Yerlitas, Serra Ilayda; Zararsiz, Gozde Ertuk; Dogan, Kubra; Kablan, Demet; Bagci, Gokhan; Ozer, SeldaThis study investigated the intricate interplay between Crimean-Congo hemorrhagic fever virus infection and alterations in amino acid metabolism. The primary aim is to elucidate the impact of Crimean-Congo hemorrhagic fever (CCHF) on specific amino acid concentrations and identify potential metabolic markers associated with viral infection. One hundred ninety individuals participated in this study, comprising 115 CCHF patients, 30 CCHF negative patients, and 45 healthy controls. Liquid chromatography-tandem mass spectrometry techniques were employed to quantify amino acid concentrations. The amino acid metabolic profiles in CCHF patients exhibit substantial distinctions from those in the control group. Patients highlight distinct metabolic reprogramming, notably characterized by arginine, histidine, taurine, glutamic acid, and glutamine metabolism shifts. These changes have been associated with the underlying molecular mechanisms of the disease. Exploring novel therapeutic and diagnostic strategies addressing specific amino acids may offer potential means to mitigate the severity of the disease.Öğe An Investigation of the Effects of Curcumin on the Changes in the Central Nervous System of Rats Exposed to Aroclor 1254 in the Prenatal Period(BENTHAM SCIENCE PUBL, 2018) Alcigir, Mehmet Eray; Dogan, Halef Okan; Dikmen, Begum Yurdakok; Dogan, Kubra; Vural, Sevil Atalay; Yilmaz, Fatma Meric; Isgoren, AtillaBackground & Objective: Aroclor 1254 is a widespread toxic compound of Polychlorinated Biphenyls (PCBs), which can create significant nervous problems. No remedies have been found to date. The aim of this study was to reveal the damage that occurs in the central nervous system of rat pups exposed to Aroclor 1254 in the prenatal period and to show the inhibiting effect of curcumin, which is a strong anti-oxidant and neuroprotective substance. Method: The study established 3 groups of adult female and male Wistar albino rats. The rats were mated within these groups and the offspring rats were evaluated within the group given Aroclor 1254 only (n=10) and the group was given both Aroclor 1254 and curcumin (n=10) and the control group (n=10). The groups were compared in respect of pathomorphological damage. The immunohistochemical evaluation was made of 8-hydroxdeoxyguanosine (8-OHdG), 4-hydroxynoneal (4HNE), myelin basic protein (MBP) expressions and TUNEL reaction. The biochemical evaluation was made of the changes in the TAS-TOS and Neuron Specific Enolase (NSE) levels. Damage was seen to have been reduced with curcumin in the 8OHdG and TUNEL reactions, especially in the forebrain and the midbrain, although the dosage applied did not significantly change TAS and TOS levels. Consequently, it was understood that Aroclor 1254 caused damage in the central nervous system of the pup in the prenatal period, and curcumin reduced these negative effects, particularly in the forebrain and the midbrain. Conclusion: It was concluded that curcumin could be a potential neuroprotective agent and would be more effective at higher doses.Öğe Level of Serum Adiponectin in Sjogren's Syndrome(Erciyes Univ Sch Medicine, 2019) Dogan, Kubra; Urhan, Muhammad Emre; Yildiz, Seymanur; Derin, Emin; Sahin, Ali; Dogan, Halef OkanObjective: To evaluate the serum adiponectin level and determine the association between adiponectin and various clinical and laboratory findings in patients with primary Sjogren's syndrome (pSS). Materials and Methods: A total of 50 patients and 30 healthy volunteers were enrolled in the present study. Serum adiponectin levels were detected by colorimetric enzyme-linked immunosorbent assay. The medical history of patients including complete blood count analysis; high sensitive C-reactive protein; erythrocyte sedimentation rate (ESR); complement component 3; complement component 4; low density lipoprotein cholesterol; triglyceride; immunoglobulin G (IgG), IgA, and IgM levels; and the status of Ro 60, Ro 52, Sjogren's syndrome A, Sjogren's syndrome B, and rheumatoid factor were obtained from laboratory information system. Results: Serum adiponectin levels were 2.34 (0.77-4.95) ng/mL and 1.73 (0.01-7.76) ng/mL in patients and controls, respectively (p=0.316). Positive correlation was observed between the values of serum adiponectin, ESR (p=0.013, rho=0.362), and body mass index (p=0.018, rho=0.362) in patients. Conclusion: These findings indicate that adiponectin does not play a crucial role in the immunological and clinical patterns of pSS.Öğe Predictive role of non-viral leukocyte parameters in tracking the development and progression of Crimean-Congo hemorrhagic fever(Academic Press Ltd- Elsevier Science Ltd, 2025) Kiymaz, Yasemin Cakir; Bolat, Serkan; Hasbek, Mursit; Buyuktuna, Seyit Ali; Ipekten, Funda; Zararsiz, Gokmen; Dogan, KubraThis study aims to investigate the extended leukocyte parameters, known as cell population data (CPD), in Crimean-Congo hemorrhagic fever (CCHF) and to evaluate their diagnostic performance in assessing mortality risk and intensive care unit (ICU) admission requirements. The study included a total of 260 participants, comprising 200 individuals diagnosed with CCHF and 60 healthy controls. CPD analyses were conducted using a Sysmex-XN 9000 complete blood count analyzer. Elevated levels of immature granulocyte (IG) percentage, neutrophil reactivity indices (Ne-X, Ne-Y), dispersion of neutrophil NE-SSC and NE-SFL signals (Ne-WX, Ne-WY), monocyte metabolic activity (Mo-X), and lymphocyte metabolic activity (Ly-X) were observed in CCHF patients compared to controls. Conversely, lymphocyte metabolic activity (Ly-Y) and monocyte fluorescence intensity (Mo-WY) were lower in patients than in controls. In discriminating mortality, IG#, IG%, Ne-WY, and Ly-Y exhibited higher AUC values than other CPDs. Additionally, for predicting ICU requirements, IG#, IG%, and Ne-WY demonstrated higher AUC values compared to other CPDs. IG, Ne-WY, and Ly-Y have the potential to serve as biomarkers for predicting mortality in CCHF. Furthermore, IG and Ne-WY may emerge as innovative and reliable biomarkers for identifying severe cases and predicting ICU admission requirements.Öğe The effect of smoking and electronic cigarettes on rat testicles(Elsevier Espana Slu, 2023) Saygin, Huseyin; Korgali, Esat; Koc, Tulay; Dogan, KubraObjective: After the negative effects of smoking on public health were proven, smoking cessation campaigns were initiated by health ministries and non-governmental organizations. Many drugs have been tried to reduce the addiction to smoking and the nicotine contained in it. Recently, e-cigarettes (EC) are widely used for smoking cessation efforts, although the effects and possible harms are not fully known. In our study, we planned to show the effect of cigarette and EC smoke on the male urogenital system. Methods: Adult male wistar rats were exposed to cigarette and EC smoke in a specially designed glass bell jar. Urine cotinine levels, testicular weights, gonadosomatic index, sperm count and sperm motility, testicular histology, and biochemical findings were compared with the control group. Results: In some rats in the cigarette and EC group, the seminiferous tubules were disorganized, and the germ cells and Sertoli cells were separated and shed. Stopped germ cell separation, cavity formation, necrosis, fibrosis, and atrophy were observed in severe cases. Higher PCO levels were found in the cigarette group compared to controls. Tissue homogenates levels of LPO were higher in both EC and cigarette groups compared to controls. No significant differences were observed between groups in terms of sperm motility and sperm count. Conclusion: Cigarette and EC liquid can increase oxidative stress as well as cause morphological changes in the testicle. To be a safe option in smoking cessation studies, its effect on people needs to be enlightened. (c) 2023 Asociacion Espanola de Androlog ' ia, Medicina Sexual y Reproductiva. Published by Elsevier Espana, S.L.U. All rights reserved.Öğe Urine iodine deficiency in pregnant women living in Sivas(DERMAN MEDICAL PUBL, 2018) Dogan, Halef Okan; Karakaya, Ozgur; Dogan, Kubra; Karakus, Savas; Yildiz, Seyma NurAim: The study aimed to assess the iodine status and maternal thyroid function in the pregnant women in Sivas that is a city in central Turkey. Material and Method: One hundred-ninety-three pregnant women in their second trimester who attended the hospital for routine antenatal care were included this study. Morning spot urine samples were collected in deiodized test tubes from the study population. Urine iodine levels were determined by colorimetric modified Sandell-Kolthoff reaction. Serum free triiodothyronine (fT3), free thyroxine (fT4) and thyrotropin (TSH) levels were measured by electrochemiluminescence immunoassays. Results: Median urine iodine levels were lower in Sarkisla, Susehri Gurun, Divrigi and Kangal than Sivas Center. Median urine iodine deficiency levels of pregnant women living in Sarkisla, Susehri Gurun, Divrigi and Kangal indicated mild iodine deficiency. Discussion: Iodine deficiency is a significant problem in Sivas. Therefore, there is need for a policy such as iodine prophylaxis for pregnant women living in Sivas to eliminate this problem.
