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Öğe Antitumoral effects of Allium sivasicum on breast cancer in vitro and in vivo(SPRINGER, 2013) Tepe, Bektas; Tuncer, Ersin; Saraydin, Serpil Unver; Ozer, Hatice; Sen, Metin; Karadayi, Kursat; Inan, Deniz Sahin; Karadayi, Sule; Polat, Zubeyde; Akpulat, Askin; Duman, Mustafa; Koksal, Binnur; Turan, MustafaThis work aims to investigate the antiproliferative properties of Allium sivasicum (AS) on breast cancer. AS extracts were studied for cytotoxicity against the breast cancer cell lines. In vitro apoptosis studies of breast cancer cells were performed by annexin V staining in flow cytometry analyses. AS showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in AS treated cell lines were higher than the untreated control cells. The expressions of caspase-7 protein and TUNEL positive cells were much higher for the rats treated by AS, compared with the untreated control group. The expressions of the Ki-67 decreased in treatment groups compared with the control group. In vivo studies showed that mean tumor volume inhibition ratio in AS treated group was 38 % compared with the untreated rats. These results indicate that A. sivasicum has antitumoral potential against breast cancer.Öğe Antitumoral Effects of Melissa officinalis on Breast Cancer in Vitro and in Vivo(ASIAN PACIFIC ORGANIZATION CANCER PREVENTION, 2012) Saraydin, Serpil Unver; Tuncer, Ersin; Tepe, Bektas; Karadayi, Sule; Ozer, Hatice; Sen, Metin; Karadayi, Kursat; Inan, Deniz; Elagoz, Sahande; Polat, Zubeyde; Duman, Mustafa; Turan, MustafaBackground: There is a long standing interest in the identification of medicinal plants and derived natural products for developing cancer therapeutics. Here we investigated the antiproliferative properties of Melissa officinalis (MO) from Turkey on breast cancer. Methods: MO extracts were studied for cytotoxicity against breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). In vitro apoptosis studies were performed by annexin V staining and flow cytometry analyses. Immunohistochemistry for Ki-67 and caspase 7 in the tumoral tissue sections of DMBA-induced mammary tumors in rats was also performed, along with TUNEL assays to detect apoptotic cells. In vivo anticancer activity testing was carried out with reference to inhibition of growth of DMBA induced mammary tumors in rats. Results: MO showed cytotoxicity against three cancer cell lines, inducing increase in Annexin-positive cells. Expression of caspase-7 protein and TUNEL positive cells were much higher in rats treated by MO, compared with the untreated control group, while expression of Ki-67 was decreased. Furthermore, in vivo studies showed that mean tumor volume inhibition ratio in MO treated group was 40% compared with the untreated rats. Conclusion: These results indicated that MO extrcts have antitumoral potential against breast cancer.Öğe Comparative relaxant effects of YC-1 and DEA/NO on the sheep sphincter of Oddi(KARGER, 2006) Bagcivan, Ihsan; Kaya, Tijen; Turan, Mustafa; Karadas, Baris; Sarac, Buelent; Duman, MustafaBackground/Aims: Nitric oxide (NO) is a major inhibitor in various parts of the gastrointestinal tract. This study was designed to compare the effects of YC-1, NO-independent soluble guanylate cyclase (sGC) activator, and DEA/NO, NO-nucleophile adduct, on sheep sphincters of Oddi (SO). Methods: SO rings were mounted in a tissue bath and tested for changes in isometric tension in response to 3-(5'-hydroxymethyl- 2'-furyl)-1-benzylindazole (YC-1, 10(-10)-10(-5) M), diethylamine/NO complex (DEA/NO, 10(-8)-10(-4) M). We also evaluated the effect of YC-1 (10(-6) and 10(-5) M) and DEA/NO (10(-5) and 10(-4) M) on the levels cyclic GMP (cGMP) in isolated SO. Results: YC-1 (10(-10)-10(-5) M) and DEA/NO (10(-8)-10(-4) M) induced concentration-dependent relaxation of isolated SO rings precontracted with carbachol (10(-6) M). The pEC(50) value of DEA/NO was significantly lower than those for YC-1 (p < 0.05), with no change of E-max values. YC-1 increased cGMP levels more than control, carbachol and DEA/NO groups (p < 0.05). Conclusion: These results show that YC-1 is a more potent relaxant than DEA/NO and causes more elevation of cGMP levels in isolated SO rings. Copyright (C) 2006 S. Karger AG, Basel and IAP.Öğe Comparison of Growth Curves by Growth Models in Slow-Growing Chicken Genotypes Raised the Organic System(FRIENDS SCIENCE PUBL, 2014) Eleroglu, Hasan; Yildirim, Arda; Sekeroglu, Ahmet; Coksoyler, Fikret Nafi; Duman, MustafaTwo hundred and forty slow-growing chickens consisting of equal numbers of Hubbard S757 (S757) and Hubbard Grey Barred JA (GB-JA) strains were utilized for the investigation in organics system and were used to estimate growth curve in Gompertz and Logistic model. The asymptotic weights for GB-JA and S757 genotype female; male in the Gompertz model were estimated 3725.34 g; 6109.60 g and 4876.10 g; 6496.47 g and same parameter were found in Logistic model 2133.33 g; 2906.35 g and 2790.37 g; 3635.00 g respectively. The Gompertz model was higher estimate than Logistic model for the asymptotic weights parameter. The instantaneous growth rate for GB-JA and S757 genotype female; male in the Gompertz model were estimated 0.1424; 0.1288 and 0.1525; 0.1495 and same parameter values were found in Logistic model 0.3753; 0.3734 and 0.3873; 0.3949 respectively. Significant difference was observed for the instantaneous growth rate parameter between GB-JA and S757 genotypes in each of models. According to the results of goodness of fit in Gompertz and Logistic growth curve models, the coefficient of determination (R-2) and adjusted coefficient of determination (adj. R-2) were detected above 0.996 in boot models for two genotype broilers. The highest value of R-2 and adj. R-2 were obtained from the Logistic model in GB-JA. The two models were all fitted the growth curves of slow-growing chicken genotypes in organic system very well, and the fitting degrees R-2 were all above 0.998; for the two models; however Logistic model was the best (0.999%). c 2014 Friends Science PublishersÖğe Comparison of meat quality and fatty acid profile in slow-growing chicken genotypes fed diets supplemented with Origanum vulgare or Melissa officinalis leaves under the organic system(TAYLOR & FRANCIS LTD, 2013) Eleroglu, Hasan; Yildirim, Arda; Isikli, Nursel D.; Sekeroglu, Ahmet; Duman, MustafaThis study was conducted to compare the meat quality and selected fatty acids profile of two different slow-growing broiler genotypes (Hubbard S757; S757 and Hubbard Grey Barred JA; GB-JA) fed diets supplemented with dry oregano (Origanum vulgare L., OV; 10 g/kg basal diets) or lemon balm leaves (Melissa officinalis L., MO; 10 g/kg basal diets) under organic housing system. It is concluded that slow-growing genotypes had no effect on L* parameter of the breast, thigh and abdominal fat meat quality. Two hundred and forty chicks were allocated randomly into 4 experimental groups according to a 2x2 factorial arrangement. Birds were raised until 98 days in order to achieve an acceptable market live weight. The b* colours of breast and thigh meat were significant different among genotypes and also a* colour of breast meat of GB-JA increased (P<0.05). Slow-growing female broilers produced a higher dry matter content and lower fat content of breast meat as compared with males. There were the higher concentrations of linoleic (C18:2n-6) acid and the lower concentrations of linolenic acid (C18:3n-3) in genotypes fed with supplemented dry oregano or lemon balm leaves diet. Sex affected total unsaturated fatty acids (UFA) composition, polyunsaturated fatty acid (PUFA) and linoleic acid, were higher in slow growing males breast meat as compared with females breast meat. These results suggested that the slow-growing genotypes might had influenced the colour of breast and thigh meat, although overall meat quality was not affected under the organic system.Öğe Effect of peritoneal lavage with chlorhexidine gluconate solution on survival and adhesion formation(Turkish Surgical Assoc, 2005) Topcu, Omer; Duman, Mustafa; Koyuncu, Ayhan; Aydin, Cengiz; Turan, Mustafa; Sen, MetinPurpose: In this experimental study; effects of chlorhexidine glucunate solution on peritoneal adhesion formation and perioperative mortality were investigated. Peritoneal Hydatidosis (PH) still remains the most important complication of hydatid cyst surgery. Peritoneal lavage with scolocidal agents and chemotherapy are suggested as a therapeutic approach in the peritoneal dissemination. Materials and Methods: Eighty male rats were divided into four equal groups. Group 1: 0.9% NaCl (Control), Group 2: 20% NaCl, Group 3: 0.4% Chlorhexidine Glucunate (Chx-Glu), and Grup 4: 0.04% Chx-Glu. Two ml solution in each group was instilled into the peritoneal cavity through a median laparotomy and aspirated 5 minutes later. Animals that survived the perioperative period were sacrificed at the fifteenth postoperative day. Adhesion formation were macroscopically graded by Nair criteria. Results: Mortality rate was not significantly different between groups (p > 0.05). Adhesion formation was significantly low in study groups compared to control group (p < 0.05). Adhesion formation was significantly low in Chx-Glu groups compared to 20% NaCl group (p < 0.05). There was no diffirence of adhesion formation between Chx-Glu groups (p > 0.05). Conclusion: Currently, intraabdominal adhesions are still important problems in the surgical area. We recommend the use of 0.04% Chx-Glu solution in the treatment of hydatid disease and Peritoneal Hydatidosis as a potent and non-toxic agent in addition to its ability for decreasing intrabdominal adhesion formation.Öğe The effects of various scolicidal agents on the hepatopancreatic biliary system(KARGER, 2006) Topcu, Omer; Aydin, Cengiz; Arici, Sema; Duman, Mustafa; Koyuncu, Ayhan; Sen, MetinBackground: Sclerosing cholangitis is a rare but dreadful complication of liver hydatid surgery. Almost all scolicidal agents were shown to be potentially associated with this complication clinically or experimentally. This experimental study was designed to examine the effects of 0.04% chlorhexidine gluconate and 95% ethanol on the hepatobiliary system and pancreas and also to compare their effects with 10% povidone iodine and hypertonic 20% saline. Materials and Methods: 50 Wistar rats were divided into equal five groups identified as: 0.9% NaCl ( control group), 0.04% chlorhexidine gluconate, hypertonic 20% saline, 10% povidone iodine, and 95% ethanol. Scolicidal agents were injected into the biliary tract through a duodenal puncture. The animals were kept for 3 months. The animals were sacrificed, and histopathological examinations of the liver, biliary tract, and pancreas were performed. Results: The most severe histopathological changes of the liver, biliary tract, and pancreas were found in the ethanol group, and the least changes were detected in the chlorhexidine gluconate group. Conclusion: This experimental study confirms the deleterious effect of 95% ethanol on hepatopancreatic biliary system when used for the percutaneous drainage of hydatid diseases. For treatment of hydatid cysts in the hepatobiliary system, 0.04% chlorhexidine gluconate should be used as scolicidal agent.Öğe Ratlarda postoperatif adezyon gelişimine karşı lornosikam ve metilen mavisinin etkileri(Cumhuriyet Üniversitesi, 2004) Duman, Mustafa; Koyuncu, AyhanÖZET Karın operasyonlarından sonra oluşan karın içi yapışıklıklar ve bunlara bağlı olarak gelişen intestinal obstruksiyonlar, cerrahlar için önemli bir problemdir. Bu yapışıklıklar sonraki operasyonlarda da cerrahi yaralanma riskini artırır. Jinekolojik hastalarda da görülen pelvik adezyonlar; kronik pelvik ağrı, infertilite gibi ciddi sorunlara neden olur. Adezyon gelişimini önlemek veya azaltmak için çeşitli adjuvanlar denenmektedir. Bu çalışma rat çekum modelinde, NSAİ ajan olan Lornoksikam ve daha önce adezyon oluşumuna karşı etkinliği gösterilmiş bir ajan olan Metilen mavisinin adezyon önleyici etkilerini araştırmak ve lornoksikam ile karşılaştırmak amacı ile yapıldı. Kontrol, serum fiyolojik, metilen mavisi ve lornoksikam gruplarına ayrılan ratlarda çekumda abrezyon oluşturulduktan sonra kontrol grubuna herhangi bir işlem yapmadan, SF grubuna sadece serum fizyolojik verilerek karınları kapatıldı. Metilen mavisi grubuna %1 lik metilen mavisinden 2 mi karın içine konularak, lornoksikam grubuna ise 0.5 ve 1/10 dilüe dozlarda lornoksikam verilerek karınları kapatıldı. Postoperatif 14. gün bütün ratlann karınları tekrar açılarak Adezyon Skor Örgütünün skorlama sistemine göre puanlar verilerek değerlendirme yapıldı. Postoperatif adezyon gelişimi karşılaştrnldığrnda lornoksikam grupları ve metilen mavisi grubu, serum fizyolojik ve kontrol grubuna göre anlamlı düzeyde etkin olarak bulundu. En iyi sonuç lornoksikam 0.5 ve metilen mavisi grubunda bulundu. Lornoksikam ve metilen mavisinin adezyon engelleyici etkileri arasında anlamlı bir fark bulunamadı. Lornoksikan ve metilen mavisinin daha kapsamlı çalışmalardan sonra klinik uygulamaya katılma potansiyellerinin yüksek olduğu düşünüldü.Öğe Small bowel tumors in emergency surgery(Turkish Association of Trauma and Emergency Surgery, 2010) Turan, Mustafa; Karadayi, Kursat; Duman, Mustafa; Özer, Hatice; Arici, Sema; Yildirir, Cihan; Koçak, OsmanBACKGROUND The aim of the present study was to describe the clinical presentation, diagnostic work-up, surgical therapy, and prognosis of 13 patients with small bowel tumor admitted for surgical procedures in an emergency setting. METHODS From 1996 to 2008, 13 consecutive surgical cases of small bowel tumors were treated at the Cumhuriyet University Faculty of Medicine, Department of General Surgery, and Kütahya State Hospital, Department of General Surgery. Clinical and radiological charts of these patients were reviewed retrospectively from the department database. RESULTS Intestinal obstruction (7 cases) and perforation (5 cases) were the most common clinical presentations, followed by intussusception (1 case). Adenocarcinoma was the most frequent histologic type (4 cases), while small bowel sarcoma was seen in three cases and non-Hodgkin lymphoma in two cases. The remaining cases had carcinoid tumor, small bowel angioleiomyoma, Brunner's gland adenoma, and inflammatory pseudotumor of the small intestine. CONCLUSION Small bowel tumors are rare, the symptoms often non-specific, and the accuracy of different diagnostic tests remains to be improved. Timing and type of the intervention to the process and biological behavior of the pathological cells predict the prognosis.Öğe THE EFFECT OF DRY CAPER (Capparis spinosa) FRUIT ON EGG PRODUCTION AND QUALITY CHARACTERISTICS OF LAYING HENS(Univ Agriculture, Fac Veterinary Science, 2014) Yildirim, Arda; Sekeroglu, Ahmet; Koc, Huseyin; Eleroglu, Hasan; Tahtali, Yalcin; Sen, Merve Isil; Duman, MustafaThis study was conducted to determine the effect of increasing dietary inclusion of dry Caper (Capparis spinosa) fruit (DCF) on egg production and quality characteristics of laying hens between 20 and 32 weeks of age. Four groups of commercial hens (ATAK-S) were fed with diets containing 0, 5, 10 and 15 g DCF/kg. The results showed that final body weight, feed intake, shape index (SI) and morning hen-day egg yield were influenced by dietary supplementation of DCF (P<0.05). Henday egg production (HDP) value of commercial hens groups was 73.47%, 56.67%, 54.18% and 51.72% respectively. The effect of increased the levels of DCF on egg weight, specific gravity, shell breaking strength, shell thickness, surface area, albumen pH, albumen index (AI), yolk index (YI), shell weight and haugh unit (HU) was not statistically significant (P>0.05) during the entire experiment. The lightness (L*) and redness (a*) values for egg shell color were similar (P>0.05) in the DCL supplemented groups as compared to the control group. The yellowness (b*), Hue angle (H), chroma (C*) and Delta E* values were lower (P<0.05) in 15 g DFC/kg group when compared with the control. Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and total phenolics amount of DCF were 43.75 +/- 0.680 mmol trolox/kg, 60.03 +/- 3.710 mmol TEAC/kg and 3.16 +/- 0.060 g gallic acid equivalents (GAE)/kg, respectively. Based on the results from the current study, the dietary supplementation with dry DCF had adverse effects on productivity performance traits and egg quality.Öğe The welfare of slow growing broiler genotypes reared in organic system(UNITED ARAB EMIRATES UNIV, 2015) Eleroglu, Hasan; Yildirim, Arda; Duman, Mustafa; Sekeroglu, AhmetThis study study was conducted to compare welfare indicators and some serum biochemical parameters of two different slow-growing genotypes (Hubbard S757; S757 and Hubbard Grey Barred JA; GB-JA) under organic housing system. A total of 240 day-old chicks were allocated randomly into 2 experimental group with 6 replicates and 20 chicks was reared in each replicate. The tonic immobility was a significant difference between genotypes and sex at 10 and 14 weeks, respectively (P < 0.05). Overall duration of tonic immobility was similar (P > 0.05) in genotypes and sex. The effect of sex was significant for width of tarsometatarsus at the joint with tibiotarsus; males had greater fluctuating and relativite asymmetry than females (P < 0.05). There were positively correlations between fluctuating and relative asimetry values of the third phalanx of the mid toe length and the fourth phalanx of the outer toe length from specific feet trait measurements. Serum cholesterol and uric acid had significant differences between genotypes (P < 0.01). Cholesterol levels was significantly (P < 0.01) higher in male than in female slow-growing broiler. It is concluded that slow-growing genotypes and sex had no effect on measures of welfare, such as the fluctuating asymmetry, relativite asymmetry, tonic immobility and rectal temperatures.
