Yazar "Dumanli, Nazir" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • Küçük Resim Yok
    Öğe
    First Molecular Detection ofDirofilaria immitisandD. repensin Dogs from Kyrgyzstan
    (Springer Int Publ Ag, 2020) Aydin, Mehmet Fatih; Altay, Kursat; Aytmirzakizi, Ayperi; Dumanli, Nazir
    Background Dirofilaria immitisandDirofilaria repensare the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan. Purpose The goal of this study was to investigateDirofilariaspecies using PCR and sequencing in dogs from Kyrgyzstan. Method Dirofilariaspp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan. Result The overall prevalence ofDirofilariaspp. was 0.59% (2/337): DNA ofD. immitiswas detected in one sample and DNA ofD. repensin second positive sample. In second sample, parallel co-infection ofD. repenswithWolbachiawas also found. WhileD. immitissequence showed 98.70-100% similarity with previously reported sequences ofD. immitisfrom dog blood,D. repensshared 100% identity with other sequences ofD. repens. Conclusion These results provided first evidence forDirofilariaspp. in Kyrgyzstan and emphasized the veterinary and medical importance.
  • Küçük Resim Yok
    Öğe
    First Molecular Evidence for Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum in Asymptomatic Shelter Dogs in Kyrgyzstan
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2020) Altay, Kursat; Aydin, Mehmet Fatih; Aytmirzakizi, Ayperi; Jumakanova, Zarima; Cunusova, Ayday; Dumanli, Nazir
    Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) have been investigated using species specific PCR and sequencing in 170 dogs from Kyrgyzstan. Maximum likelihood estimation (MLE) of the infection rates with 95% confidence intervals (CI) was calculated. The molecular prevalence of hemoplasma infection was 5.29% (CI 2.57-9.34). It was found that, five (2.94%, CI 1.06-6.22) samples were found to be infected with Mhc, one (0.59%, CI 0.03-2.57) sample with CMhp and three (1.76%, CI 0.44-4.52) samples with both species. These results demonstrate that dogs can be exposed to each haemoplasma species and provide first molecular evidence for these species in Kyrgyzstan.
  • Küçük Resim Yok
    Öğe
    First Molecular Evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in Dogs from Kyrgyzstan
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023) Altay, Kursat; Erol, Ufuk; Sahin, Omer Faruk; Aydin, Mehmet Fatih; Aytmirzakizi, Ayperi; Dumanli, Nazir
    Tick-borne parasitic diseases cause mild to severe infections among vertebrate hosts, including dogs. Species in the genus Babesia are important tick-borne pathogens and have worldwide distributions. Although there are data on the prevalence and distribution of Babesia species among dogs around the world, there is no information available in Kyrgyzstan, according to a literature review. In this study, 337 dogs were screened by nested PCR for the presence of the 18S small subunit ribosomal RNA (18S SSU rRNA) gene of piroplasm species. Overall prevalence was 6.23% (21/337) for Babesia/Theileria spp. DNA sequencing of positively tested samples revealed that eighteen samples were infected with Babesia vogeli (B. vogeli) (5.34%), two samples with B. vulpes (0.59%), and one sample with Theileria ovis (T. ovis) (0.29%). The phylogenetic analyses and nucleotide sequences in contrast with those present in GenBank revealed that two nucleotide substitutions (594th and 627th) were found between B. vogeli isolates, including ours, indicating that the mutation is relatively rare. The sequences of other pathogens obtained in this study confirmed 100% nucleotide identity with B. vulpes and T. ovis sequences in GenBank. To the best of our knowledge, B. vogeli, B. vulpes, and T. ovis were detected for the first time in dogs from Kyrgyzstan, and it is thought that results will contribute to the understanding of the epidemiology of canine tick-borne pathogens in the country. © 2023 by the authors.
  • Küçük Resim Yok
    Öğe
    A molecular and parasitological survey of Hepatozoon canis in domestic dogs in Turkey
    (ELSEVIER SCIENCE BV, 2015) Aktas, Munir; Ozubek, Sezayi; Altay, Kursat; Balkaya, Ibrahim; Utuk, Armagan Erdem; Kirbas, Akin; Simsek, Sami; Dumanli, Nazir
    In this study, asymptomatic dogs in nine provinces of Turkey were surveyed to investigate the prevalence and intensity of Hepatozoon canis infection. DNA obtained from blood samples collected from 694 domestic dogs (243 stray, 288 shelter, and 163 pets) of both genders and varying ages were evaluated by polymerase chain reaction (PCR). In addition, 285 thin blood smears prepared from these blood samples were also evaluated for microscopic examination. Direct microscopy revealed Hepatozoon gamonts in the peripheral blood of three of 285(1.0%; 95% confidence interval (CI): 0.21-3.04) tested. Using PCR, 155 of the 694(22.3%; 95% CI: 19.28-25.61) were found to be positive for the presence of H. canis DNA. The prevalence of infection was higher in adult dogs (26.2%; 95% CI: 22.1-30.7) than young animals (16.4%; 95% CI: 12.2-21.3). Although the prevalence determined by PCR was higher in male dogs (24.5%; 95% CI: 19.6-29.9) than in female dogs (20.8%; 95% CI: 16.9-25.1), gender differences were not significant. Pet dogs had a lower prevalence of infection (10.4%; 95% CI: 6.2-16.2) compared to stray (26.3%; 95% CI: 20.9-32.3) and shelter dogs (25.7%; 95% CI: 20.7-31.1), but no significant association between stray and shelter dogs was found for the presence of the parasite. Partial sequences of the 18S ribosomal RNA (rRNA) gene shared 99-100% similarity with the corresponding H. canis isolates. This epidemiological survey revealed a high prevalence of H. canis in dogs from several provinces in Turkey, and it suggests that the age and origin are associated with the parasite. (C) 2015 Elsevier B.V. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from Turkey
    (BMC, 2015) Aktas, Munir; Ozubek, Sezayi; Altay, Kursat; Ipek, Neval Duygu Sayin; Balkaya, Ibrahim; Utuk, Armagan Erdem; Kirbas, Akin; Simsek, Sami; Dumanli, Nazir
    Background: Canine tick-borne parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. In addition to their veterinary importance, domestic dogs play an important role in the transmission cycles of some agents by acting as reservoirs and sentinels. This study investigated Babesia, Theileria, Anaplasma, and Ehrlichia species in asymptomatic dogs in ten provinces of Turkey. Methods: DNA obtained from blood samples collected from 757 domestic dogs (243 stray, 351 shelter, 163 pet) of both sexes and various ages were evaluated using PCR and reverse line blotting (RLB) assays. Results: Of the 757 dogs tested, 41 (5.4%) were found to be infected with one or more parasites. Ehrlichia canis (37/757, 4.9%) was the most common canine tick-borne pathogen, followed by Anaplasma platys (4/757, 0.5%). Babesia canis and Theileria annulata were each detected in 1 (0.13%) sample. Combined infection of E. canis and A. platys was detected in 2 (0.3%) samples. The prevalence of tick-borne pathogens was higher in adult dogs (6.8%) than in those under one year old (3.1%). Difference in infection rate of male and female dogs was not significant. Pet dogs had a lower prevalence of infection (1.2%) compared to stray (7.4%) and shelter dogs (6%) although the difference between stray and shelter dogs was not significant. Conclusions: Babesia canis, T. annulata, A. platys, and E. canis species were identified at the molecular level in dogs in several provinces of Turkey, with E. canis being the most common species among tick-borne pathogens. Detailed studies should be conducted regarding the existence and prevalence of B. canis and Dermacentor reticulatus in eastern Turkey.