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Öğe Antiinflammatory and antioxidant activities of different hemostatics and pulp capping materials applied on rat teeth Biochemical effects of direct pulp capping with different hemostatics(Bayrakol Medical Publisher, 2021) Saygin, Aysegul Goze; Unal, Murat; Ercan, Nazli; Demir, PinarAim: This study aimed to investigate the anti-inflammatory and antioxidant activities of different hemostatic agents and pulp capping materials. Material and Methods: Ninety-six Wistar albino mate rats were divided into the following groups: Sterile Saline (SS), Sodium Hypochlorite (NaOCl), Mecsina Hemostopper (MIAS). Occlusal cavities were prepared in the first molar teeth. Hemostatic agents were used to control bleeding and different pulp capping materials were applied on the exposed pulp area. Subgroups were created according to pulp capping materials (Dycal, Biodentine Theracal, MTA RepairHP). Half of the groups were left to wait for sacrifice on the 7th day and the others on the 28th day. Cardiac blood was taken to determine of antiinflammatory and antioxidant serum markers. Results: The use of different hemostatic agents in terms of antioxidant (CAT, GPX) and anti-inflammatory (IL1-beta, TNF-alpha, IL-6) activity was statistically significant (p <0.05). There was no statistical significance between groups for SOD (p> 0.05). IL-6 was significantly higher on the 7th day than on the 28th day (p <0.05). The and-inflammatory activity reduced from the 7th to the 28th day, in contrast, antioxidant activity induced. Discussion: The inflammation process was related to the hemostatic agents and the capping materials used in DPC treatment. MHS may be an alternative for DPC treatment for bleeding control.Öğe Determination of 8-hydroxy-2?deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour(Ankara Univ Press, 2020) Ercan, Nazli; Yuksel, Murat; Kockaya, MustafaCanine transmissible venereal tumour (TVT) is a contagious cancer and sexually transmitted one dog to another by allogenic transfer of living cancer cells. The aim of this study is to examine the relationship between antioxidative metabolism and venereal tumour at Kangal Dogs and to show the size of oxidative damage it causes through 8-hydroxy-2'deoxyguanosine (8-OHdG). The materials of this study included the blood sera of 15 Kangal Dogs with TVT, and as a control group of 15 healthy Kangal Dogs. The sera levels of 8-OHdG were determined by competitive enzyme linked immunosorbent assay (ELISA) method. The level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) which are antioxidative enzymes were investigated by spectrophotometric methods as described. Serum 8-OHdG and MDA levels of dogs with TVT were found to be significantly increased compared to the control group (P<0.001). The levels of GPx were statistically different (P<0.05). In the light of the findings, which are obtained in this study, the transmissible venereal tumour, which is an infectious and treatable type of cancer, can lead to new studies that may be caused by oxidant-antioxidant metabolism.Öğe Diagnostic value of serum procalcitonin, neopterin, and gamma interferon in neonatal calves with septicemic colibacillosis(SAGE PUBLICATIONS INC, 2016) Ercan, Nazli; Tuzcu, Nevin; Basbug, Onur; Tuzcu, Mehmet; Alim, AhmetThe objective of our study was to compare the serum concentrations of procalcitonin (PCT), neopterin, tumor necrosis factor alpha (TNF-), prostaglandin E-2, malondialdehyde, interleukin 8, and gamma interferon (IFN-) in neonatal calves with septicemic colibacillosis (n = 15) with healthy controls (n = 15). Septicemic colibacillosis was diagnosed based on clinical signs and the isolation of Escherichia coli from heparinized blood in clinically suspected cases. PCT, neopterin, TNF- (p < 0.05), and IFN- concentrations in calves with septicemic colibacillosis were significantly higher than in the healthy controls (p < 0.01). Serum PCT concentrations were similar to 4 times higher in the calves with septicemic colibacillosis than in the healthy calves, suggesting that PCT could be a useful marker of septicemic colibacillosis in neonatal calves.Öğe Early prognostic markers to predict unsuccessful pregnancy in dairy cattle(Wiley, 2024) Yokus, Beran; Takci, Abdurrahman; Ercan, Nazli; Em, Bernan; Uysal, ErsinThis study aimed to investigate maternal serum levels of some angiogenic factors and certain proteins in dairy cattle for (1) early prediction of unsuccessful fertilization and (2) early detection of possible pregnancy failures (early EM) after positive insemination Serum samples were collected from the same cattle at three distinct time points: 30 days before artificial insemination (B-AI), on the day of artificial insemination (AI), and 30 days after artificial insemination (A-AI). As a result of the pregnancy examination, the cows were divided into two main groups according to whether they were pregnant. The results showed that leucyl/cystinyl aminopeptidase (LNPEP) concentration was significantly decreased B-AI and Secreted frizzled-related proteins (SFRP-3), Vascular Endothelial Growth Factor (VEGF) and LNPEP levels were significantly decreased on day of AI, while PRL level was increased, and these data have prognostic significance as early indicator of the risk of potentially failed pregnancy. Additionally, a significant decrease in LNPEP, SFRP3, and VEGF levels, along with an increase in PRL levels was also observed in A-AI. These results suggest that these biomarkers can be used as a screening test to monitor the course of pregnancy. There were no significant differences in serum levels of Insulin-Like Growth Factor 2 (IGF-2), Tissue inhibitors of metalloproteinases (TIMP-1), angiopoietin (ANG), Endoglin (ENG), Fibroblast growth factor (FGF), Inhibine-A (INH-A) and Transforming growth factors-beta 1 (TGF-beta 1) between the evaluated periods neither unsuccessful nor the successful pregnancy groups. This is the first study reporting that the maternal serum levels of LNPEP, SFRP3, VEGF, and PRL have important roles in pregnancy success and may indicate whether insemination outcome will be successful B-AI and predict the risk of unsuccessful pregnancy after AI in dairy cattle. The increase in such studies will allow the development of more specific, practical, and applicable markers.Öğe Effect of hesperidin supplementation on blood profile, antioxidant capacity, intestinal histomorphology and fecal microbial counts in Japanese quails(Inifap-Cenid Parasitologia Veterinaria, 2023) Ozbilgin, Abdullah; Mogulkoc, Mahmut Niyazi; Baycumendur, Fusun Erhan; Ercan, NazliThis study was conducted to determine the effects of hesperidin, a flavonoid added to quail diets, on blood serum, enzymes in tissues, intestinal histomorphology and fecal microflora. In the study, first treatment [(control) (0g hesperidin/kg feed)], second treatment [(HES1) (1 g hesperidin/kg feed)], third treatment [(HES2) (2 g hesperidin/kg feed)] was added to with the basal diet through 35 d. The study was carried out with 3 main groups, 20 quails with 5 sub-repeats in each group and a total of 300 quails. At the end of the study, blood, liver and thigh muscle tissue and fecal samples were taken. Alanine transaminase (ALT) and lactate dehydrogenase (LDH) decreased in the HES1 group but increased in the HES2 group compared to the control group (P<0.05). Aspartate transaminase (AST) increased in the HES1 group compared to the control group and decreased in the HES2 group (P<0.05). Amylase, on the other hand, showed a regular increase in HES1 and HES2 groups to which hesperidin was added to the control group in hesperidin added groups (P<0.05). The tissue antioxidant GSH, CAT and SOD enzyme parameters showed a significant increase in the hesperidin added groups compared to the control group, and this increase was found to be significant compared to the control group (P<0.05). In intestinal histomorphology, in hesperidin treatment groups increased the height of villus in the cecum tissue; in colon tissue, it was determined that hesperidin added groups increased villus height but decreased crypt depth (P<0.05). Consequently, diets hesperidin with treatments positively is thought to affect the lipid, thigh, liver and serum antioxidant enzyme levels, intestinal histomorphology and fecal microflora in quail.Öğe Effect of topical sildenafil on wound healing and oxidative stress in rats(Elsevier Sci Ltd, 2024) Tunc, Arda Selin; Ercan, NazliBackground: In this study, the effects of topical sildenafil applications on oxidative damage levels and antioxidative metabolism and their contribution to wound healing and treatment were investigated. Materials and Methods: A total of 24 healthy male rats aged 16-18 weeks, each weighing 200-250 g, were randomly divided into three groups: Group A received a saline solution, Group B received an epithelializing cream, and Group C received sildenafil cream. Following skin preparation and anesthesia, 6 mm diameter punch biopsies created wounds on the rats' backs. The treatment protocol involved daily topical dressing for seven days, after which tissue and blood samples were collected for analysis. Tissue samples underwent histopathological examination, while malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase activities in wound tissue and blood samples were measured. Results: The wound surface area created by the punch decreased in all groups by the end of the seventh day; However, the degree of wound healing differed in favor of the sildenafil cream group. Histopathologically, according to Greenhalgh's Modified Wound Healing Scoring System, all findings were graded. In the Anova test, the differences between glutathione peroxidase, catalase, and malondialdehyde levels in the serum and tissue of rats was statistically significant (P < 0.05), whereas superoxide dismutase levels were not statistically significant (P > 0.05). In the Bonferroni test, the serum CAT levels between groups A and C (P = 0.003), between groups B and C (P = 0.035), and the serum MDA levels between groups A and B (P = 0.018) and between groups A and C (P = 0.001) were found to be significant statistically. By the way, the results between tissue CAT levels in the B and C groups (P = 0.020) and between tissue GPx levels (P = 0.001) in all groups were also significant statistically. Conclusions: The study findings indicated that topical application of sildenafil led to noteworthy alterations in serum and tissue antioxidative metabolism as well as oxidative damage levels among rats with induced wounds. Sildenafil may be useful in wound treatment; it has been concluded that it is capable of directing new studies to be carried out.Öğe Effects of Lactobacillus Rhamnosus on dental implant osseointegration in osteoporotic rabbit model(Bayrakol Medical Publisher, 2021) Saygin, Aysegul Goze; Gorler, Oguzhan; Yildirim, Ali; Ercan, Nazli; Goze, Omer FahrettinAim: The effect of Lactobacillus rhamnosus (LR), probiotic bacteria that manage inflammatory disease, on osseointegration is unknown. The study aimed to evaluate the effects of LR administration on osseointegration in experimental osteoporosis induced by ovariectomy. Material and Methods: Sixteen New Zeland female rabbits were randomly divided into the following groups: SHAM (SH), SHAM and LR (SH+LR), Ovariectomy (OVX), OVX and LR (OVX+LR). Animals in the OVX group were subjected to bilateral ovariectomy to create artificial osteoporosis. After 16 weeks, a titanium dental implant was bilaterally implanted into each tibia of the animals. Animals in the SH+LR and OVX+LR groups were given oral probiotics. All animals were sacrificed and all tibia bones were separated at postoperative 8 weeks after implantation. Cardiac blood was taken to determine biochemical markers. Resonance frequency analysis (RFA) and bone-to-implant contact area (BIC) were administered. Results: RFA and BIC area were statistically significant for osseointegration in OVX+LR compared with OVX (p<0.05). The BIC area in the OVX group was statistically lower (p<0.05). ALP, BALP, Trap5b and MPO were highest in OVX groups(p<0.05). Discussion: This study concluded that osseointegration may be improved by LR administration in an estrogen-deficient state. The success rate of the implant may be increased by using probiotics in patients with osteoporosis.Öğe The Evaluation of Important Biomarkers in Healthy Cattle(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2014) Ercan, Nazli; Tuzcu, Nevin; Basbug, Onur; Gok, Kurtulus; Isidan, Hakan; Ograk, Yusuf ZiyaIn this study the aim is to determine the blood serum levels of biological markers as procalcitonin, neopterin, TNF-alpha, MDA, PGE2, IL8, IFN-gamma which are considered as highly beneficial on diagnosing the infections in the veterinary medicine and evaluating the prognosis in the healthy cattle at different ages and in different gender. The materials of this study are 48 (25 female and 23 male) cattle and calf bred (neonatal <1 month, young 12-24 month and mature >24 month) in operations in Sivas region and which are determined to be healthy via the biochemical and hematological findings. Serum procalcitonin level was found lower in neonatal group than the young and adult group (P<0.05). It was realized that neopterin level is reasonably higher in neonatal group than both young and adult group (P<0.05). MDA level in the young and adult group was measured as higher than of the neonatal group (P<0.05). There was found between the adult group and neonatal group a statistically reasonable difference for the serum PGE2 levels (P<0.05). In IL-8 level, there was found a statistically important difference only between young group and neonatal group (P<0.05). There was found no statistic difference among the levels of procalcitonin, neopterin, TNF-alpha, IFN-gamma, MDA, PGE2, IL-8 between the sexes. As a result, it is concluded that determining the levels of markers used in defining the prognosis of the infection in healthy cattle at different ages would be a base data for further studies.Öğe Lipid peroxidation level and antioxidant enzyme activities in septicemic calves(ANKARA UNIV PRESS, 2017) Meral, Ogunc; Ercan, Nazli; Fidanci, Ulvi RehaIn recent years, studies showed that there is a close relationship between oxidative stress and pathogenesis of the disease. The aim of the study is to determine the serum malondialdehyde (MDA) levels for lipid peroxidation status and serum glutathione peroxidase (GPx), superoxide dismutase (SOD), total bilirubin and albumin levels for antioxidant activities to find out the possible relationship between oxidative stress and septicemia in calves. The materials of this study are 40 septicemic and 13 healthy calves. No significant differences were observed for serum MDA and albumin levels between septicemic and healthy groups (p>0.05). Septicemic calves had significantly higher levels of GPx, SOD and total bilirubin as compared to the controls (p<0.001). The results obtained from this study suggest that septicemia in calves induced changes of antioxidant enzymes activity. The data obtained from this study indicates that oxidative stress might play important roles in the pathogenesis of septicemia in calves.Öğe Paraoxonase activity an indicator of complications at early stage of complicated pregnant cows(AGRICULTURAL RESEARCH COMMUNICATION CENTRE, 2017) Ercan, Nazli; Yokus, Beran; Gun, M. Can; Kochan, AkinIn healthy and complicated pregnant cows, on the 2nd and 6th months of pregnancy in order to determine the levels of maternal serum Paraoxonase 1 (PON1) activity and the possibility of complications can occur during the pregnancy might be a premise indication. Serum samples were taken at 2nd and 6th months of 252 pregnant cows at the end of their pregnancies. The cows were classified into two groups such as complicated (Abortion, Dystocia) and non-complicated. Maternal serum PON1 activity in 6th months was lower at complicated group than normally pregnant group (P=0.004; P<0.01), no difference was discovered between these groups in their 2nd month of pregnancy (P>0.05). Among the concentration of HDL, TP and globulin no statistical difference was observed between complicated, subgroups and normal births (P>0.05). Levels of PON1 in 2 and 6 months were statistically different between the groups of dystocia and normal pregnancy (P<0.01; P = 0.003), and abort and normal pregnancy (P<0.05; P=0.033). In this study, it was inferred that the evaluation of PON1 activity early indicator of complications for clinicians that might occur in further periods of pregnancy. These results showed the fact that PON1 activity can be used as a marker relatively at the early phases of pregnancy in complicated cows.Öğe Seasonal variation in the expression pattern of heat shock protein 70 and 90 in Common carp (Cyprinus carpio) from Karatas Lake, Burdur, Turkiye(Urmia Univ, 2023) Kuru, Nilgun; Oztop, Mustafa; Demirbag, Emel; Ercan, NazliOrganisms have evolved defense mechanisms to protect themselves from stressful conditions. The expression of heat shock proteins is considered a valid indication of protection from the adverse effects of hostile conditions. In this study, we used immunohistochemistry to investigate the seasonal effects of some abiotic factors on heat shock protein 70 and 90 (HSP70 and HSP90) expression in the liver, gills, and muscle tissues of 24 Common carp (Cyprinus carpio) caught in Karatas Lake (Burdur, Turkiye) using gillnets of various mesh sizes. We also measured some physicochemical parameters on-site at sampling time and took water samples for further analyses of other physicochemical parameters and heavy metals. Immunostaining for HSP90 was stronger than for HSP70 in both liver and gill samples. Liver and gill structures exhibited significant seasonal differences in HSP70 and HSP90 immunoreactivity, and the same was true for immunostaining for HSP70 and HSP90 in muscle samples. Some physicochemical properties seemed to vary considerably between seasons, with Fe, Mn, and Zn levels tending to exhibit changes throughout the seasons. However, these levels were considered acceptable for human health. In conclusion, this study suggests that substantial changes in HSP70 and HSP90 expression may be essential for seasonal adaptation and tolerance. Further research on fish HSPs would greatly contribute to aquaculture, which is essential for meeting food requirements.(c) 2023 Urmia University. All rights reserved.Öğe Serum oxytocin and lipid levels of dogs with maternal cannibalism(ELSEVIER SCIENCE INC, 2018) Kockaya, Mustafa; Ercan, Nazli; Demirbas, Yasemin Salgirli; Pereira, Goncalo Da GracaMaternal cannibalism is a condition whereby a dam consumes her own offspring after killing them. Although biological factors such as low levels of serum oxytocin and lipid levels have been shown to link to failure in maternal behaviors in different animals, underlying mechanisms responsible for abnormal maternal behavior has not yet been investigated in detail in dogs. To fill this gap, this study aimed to investigate serum lipid and oxytocin levels of the dogs having a previous history of maternal cannibalism. Mature female Kangal dogs with a history of maternal cannibalism (n = 15) and with a normal postpartum history (n = 15) were enrolled in the study. Serum oxytocin and lipid levels were significantly lower in dogs with a previous history of maternal aggression (P < 0.05). Findings of this study suggest that oxytocin is an important neuroendocrine factor in dogs for the normal onset of maternal behavior. Furthermore, low levels of high-density lipoprotein, low-density lipoprotein, and cholesterol might be contributing factors for development of abnormal maternal behavior. Thus, low levels of oxytocin and lipids should be taken into consideration in dogs with abnormal maternal behavior. (C) 2018 Elsevier Inc. All rights reserved.Öğe The effect of adding wheat and corn gluten to the diet of rats on the autoimmune and histopathological parameters in the intestine and liver(Univ Zulia, Facultad Ciencias Veterinarias, 2024) Gumus, Recep; Kapakin, Kubra Asena Terim; Kirman, Esra Manavoglu; Bolat, Ismail; Imik, Aybuke; Ercan, NazliThis study investigated the histopathological and immunohistochemical effect on the intestine and liver tissues with addition of the soybean meal (SBM), wheat Gluten meal (WGM) and Corn gluten meal (CGM) to rat diet. A total of 24 average twenty-day-old male rats (Wistar albino) were used in the study. The rats were randomly divided into 3 groups with 8 animals in each group (Control, Wheat and Corn groups). The diet provided to all three groups contained proteins, which were SBM, WGM and CGM in the Control, Wheat and Corn groups, respectively. In the study, the group fed with SBM was used as the Control group. Rats were fed a diet containing 22% crude protein and 2,598 kcalkg(-1) metabolic energy throughout the experimental period. The feeding trial was continued for a period of 50 days. Degenerative changes of varying severity in intestinal epithelial cells and atrophy in villi were observed. Similarly, the degenerative changes, especially vacuolar or hydropic degeneration were determined in hepatocytes. It was determined that the CD4 level were statistically significantly increased in the Wheat and Corn groups compared to the Control group ( P <0.01) on intestine tissue. Also, it was determined that the IgA level was statistically significantly increased of the Wheat and Corn groups in liver tissue. ( P <0.05). As a result, it was observed that the histopathological and immunohistochemical parameters of the intestine and liver tissues of the rats fed with diets containing highly WGM and CGM were limitedly affected.Öğe Urine 8-Hydroxy-2 '-Deoxyguanosine (8-OHdG) Levels of Dogs in Pyoderma(ANKARA UNIV PRESS, 2012) Ercan, Nazli; Fidanci, Ulvi RehaIn this study, it is aimed to investigate correlation between antioxidative metabolism and the significant skin disease pyoderma in dogs and to demonstrate the extent of oxidative damage with 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels. Healthy (n=13), females diagnosed as pyoderma (untreated) (n=16), heathy (n=14) males and males diagnosed as pyoderma (untreated) (n=12) consisted the four study groups. In this aspect two control and two diseased groups were formed according to gender. Blood and urine samples were obtained from all the groups and bacterial swabs were collected from the diseased groups as well. Plasma malondialdehyde (MDA) besides urine 8-hydroxy-2'-deoxyguanosine levels measured with LC/MS/MS. MDA and 8-OHdG levels were increased in diseased groups. Differences among groups were found to be significant (p <= 0.05). In conclusion in our opinion untreated pyoderma cases could result in DNA damage.
