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Öğe In Vitro Antioxidant, Antimicrobial, Anticancer Activities Assessment of Thymus pectinatus, Screening of Enzyme Inhibitory(web of Science, 2021) Ergül, Merve; Güçlü, Gülşen; Ataş, Mehmet; Çetin, Metin Durmuş; Eruygur, Nuraniye; Uçar, Özmen Esra; Akpulat, Hüseyin AşkınAccording to the data obtained in this study, Thymus pectinatus has antioxidant and enzyme inhibitory activities while has not showed antimicrobial activities. The results of the current study provide valuable information, showing that the major component of the water extract of T. pectinatus is "1,3-Propanediol, 2 -methyl-, dipropanoate" (59.34 %). This is the first investigation of the antioxidant and enzyme inhibitory activity of T. pectinatus. The results showed that the water extract had high antioxidant, anti-amylase, and anticancer effects, and a higher total phenolic content. Therefore, further phytochemical and bioactivity-guided isolation of T. pectinatus water extract could be carried out to identify the active compounds.Öğe İzole gebe sıçan myometriyumunun kasılma hareketleri üzerinde adrenerjik, purinerjik, kannabinoid ve RHOA/RHO-kinaz sistemlerine etkili ajanların yanıtlarının incelenmesi(Cumhuriyet Üniversitesi, 2015) Ergül, Merve; Saraç, BülentBu çalışmada, izole gebe sıçan myometriyumunun kasılma hareketleri üzerine çeşitli sistemler aracılığıyla etkisini gösteren ajanların yanıtlarının incelenmesi konu edilmektedir. Erken doğum dünya genelinde nedeni tam olarak anlaşılamamış ve önlenmesi için yeterli tedavi yöntemleri geliştirilememiş bir halk sağlığı sorunudur. Mevcut tokolitik ajanların etkinliği ve güvenliği ile ilgili var olan eksiklikler, önemli maternal ve fetal yan etkilerin gözlenmediği yeni uterus gevşetici ilaçların geliştirilmesini gerektirmektedir. Ritmik uterus kasılmaları doğumda önemli bir role sahiptir. Çeşitli uyarıcı ve inhibe edici yolaklar uterus gevşemesi ve kasılması arasındaki dengeyi gebelik sırasında düzenlemektedirler. Fakat her iki zıt fonksiyonel durum arasında gerçekleşen önemli değişiklikler ve etkileşimler henüz tam olarak anlaşılamamıştır. Adrenerjik, purinerjik, kannabinoid ve rhoA/rho-kinaz sistemleri üzerinden etkilerini gösteren ajanların uterus hareketleri üzerindeki etkilerinin incelenmesi erken doğumun önlenmesi için umut vaat eden yeni araştırmalar arasında yer almaktadır. Çalışmamızda; ZD-2079, ZD-7114, AS-1892802, fasudil hidroklorid, WIN 55,212-2, ACEA ve PPAD'ın gebe sıçanlardan izole edilmiş myometriyum düz kası amplitüd ve frekansını azalttıkları gösterilmiştir (p<0.05). Myometriyal kasılmalar üzerinde kontrole göre anlamlı azalma oluşturan bu maddelerin yeni tokolitik ajanların geliştirilmesine katkı sağlayabileceği düşünülmektedir.Öğe Neuroprotective effect of astaxanthin (ATX) against cognitive impairment on PTZ-induced epileptic seizures in rats and against PTZ-induced neurotoxicity in SH-SY5Y human neuroblastoma cell culture(Sivas Cumhuriyet University, 2019) Karademir, Mustafa; Gümüş, Erkan; Taştemur, Yaşar; Ergül, Merve; Ergül, Mustafa; Karabulut, Sebahattin; Akkaya, RecepObjective: Epilepsy is a common brain disorder that seizures could cause neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of this way. The aim of this study was to investigate the neuroprotective effect of astaxanthin (ATX), on pentylenetetrazole (PTZ) induced epileptic seizures in rats and in SH-SY5Y human neuroblastoma cell culture.Method: In our study, we used 42 male 230-250 g Wistar Albino rats. Animals were divided into seven groups as control, saline (PTZ; 1 ml/kg serum physiologic), positive control (2,5 mg/kg diazepam), 10 mg/kg, 20 mg/kg, 40 mg/kg and 80 mg/kg ATX for seven days. Thirty min after the administration of the last drug at the indicated doses, PTZ was administered 45 mg/kg to induce an epileptic seizure. The animals were observed for 30 min. Seizure stages according to the Racine Scale (RC) and first myoclonic jerk times (FMJ). Twenty four hours after PTZ injection, passive avoidance test was performed, and then brain tissues were removed for biochemical and histopathological evaluation. The hippocampal Cornu Ammonis 1 (CA1), CA3 and dentate gyrus (DG) regions were evaluated histopathologically regarding neuronal damage. Besides, oxidative stress markers total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI)) were measured in brain tissues. Furthermore, ATX was performed in vitro SH-SY5Y human neuroblastoma cell culture to evaluate PTZ-induced neurotoxicity.Results: When epileptic behaviors were evaluated, ATX did not affect RC and FMJ (p>0, 05). However, ATX reduced both cognitive impairment in passive avoidance test and neuronal damage in the hippocampus (p<0, 05). Moreover, ATX reduced both TOS levels and OSI in the brain (p<0, 05). Besides of these in vitro studies, ATX increased neuronal viability in vitro. Conclusions: Although ATX does not have antiepileptic properties directly, it has a protective effect on not only in vivo but also in vitro. These effects may occur by possible oxidative pathways.Öğe Pharmacological and biological features of ethanol extract of Salvia virgata Jacq.(2021) Ergül, Merve; Ataş, Mehmet; Bal, Halil; Sözmen, Esra Uçar; Eruygur, Nuraniye; Şenkal, Belgin Coşge; Uskutoğlu, TansuSalvia virgata as an ethnomedicinal plant comprehends a variety of efficient active ingredients and shows diverse pharmacological actions, such as anti-proliferative, anti-inflammatory, and antimicrobial effects. In this study we aimed to determine the biological content of the ethanolic extract of S. virgata and to determine its possible pharmacological effects. The broth microdilution technique was carried out for determining antimicrobial activities, and the test microorganisms included E. coli, S. aureus, B. cereus, P. aeruginosa, C. albicans, and C. tropicalis. DPPH and ABTS methods were used to detect antioxidant activity. The XTT cell viability test was utilized to assess the antiproliferative activity of the ethanolic extract of S. virgata on L929 and MDA-MB-231 cell lines. S. virgata exhibited reasonable antimicrobial effects against E. coli (0.312 mg/mL) and S. aureus (0.312 mg/mL). DPPH and ABTS IC50 values were determined 291.58 ± 0.004 ?g/mL, 16.74 ± 0.007 ?g/mL respectively. S. virgata ethanolic extract TPC and TFC were observed 283.35 ± 10.4 mg GAE/g and 13.37 ± 1.6 mg QE/g, respectively. The extract was screened against ?-amylase and ?-glucosidase, AChE, and BChE enzymes, and inhibition activity was determined 75.73%, 62.72%, 67.19%, 3.18% respectively. The extracts did not significantly affect the L929 cell viability, while MDA-MB-231 remarkably reduced cell viability (IC50 = 0.118 mg/mL). The ethanolic extract of S. virgata can be considered as a potential therapeutic agent in the treatment of different pathological conditions due to its antimicrobial, antioxidant, and anticancer effects.Öğe Protein pump inhibitors esomeprazole and pantoprazole increase the chemosensitivity of Cml cells against imatinib(2018) Ergül, Merve; Ergül, MustafaObjective: Proton pump inhibitors (PPIs) largely used drug to treat gastroesophageal disease such as gastric ulcers.Moreover, in recent years, several studies suggest that PPIs have important anti-cancer effect in monotherapy and orcombination with chemotherapy.The aim of this study was to investigate whether esomeprazole and pantoprazole exhibit anti-cancer effect alone orcould enhance chemosensitivity of CML cell line K562 to imatinib.Method: Human chronic myeloid leukemia (CML) cells were cultured and treated with different concentrations ofesomeprazole, pantoprazole, and imatinib alone. Also these cells exposed to imatinib + esomeprazole and imatinib +pantoprazole combinations, respectively and incubated 24 h. The antiproliferative activities of the (PPIs) alone or incombination of imatinib was evaluated using the XTT colorimetric assay.Results: According to experimental data, neither PPIs showed any cytotoxicity on the K562 cell line at allconcentrations except at 500 and 1000 ?M. However, when combined with imatinib separately, they were found to havesignificant anti-cancer effects on K562 cells when compared to cell lines treated with imatinib alone (p<0.05).Conclusions: Taken together, the inhibition of V-ATPase via esomeprazole and pantoprazole might enhance thechemosensitivity of imatinib in CML cells. However, further studies are needed to be able to utilize PPIs in CML.
