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Öğe Adenosine deaminase and xanthine oxidase levels in multiple sclerosis patients(Bayrakol Medical Publisher, 2020) Gokce, Seyda Figul; Demirpence, Ozlem; Cigdem, Burhanettin; Bolayir, Asli; Ersan, SerpilAim: Multiple sclerosis represents an autoimmune, chronic, inflammatory, and demyelinating disease of the central nervous system. Inflammation and oxidative stress are considered to take a significant part in its pathogenesis. Adenosine deaminase (ADA) and xanthine oxidase (XO) enzymes, which are involved in purine metabolism, are critical for regulating inflammation and oxidative stress. Therefore, this study's goal is to evaluate the levels of these two enzymes in patients with the relapsing-remitting type of multiple sclerosis (MS) in their remission period. Material and Methods: Thirty patients with relapsing-remitting multiple sclerosis (RRMS) who were in their remission period and diagnosed in accordance with the Mc Donald 2010 criteria along with 30 healthy volunteer controls, matched with regard to age and gender, were enrolled in the research. Serum ADA levels were studied by the sensitive colorimetric method that was defined by Giusti, while XO levels were studied by the Worthington method. Results: RRMS patients had significantly higher serum ADA and XO levels compared to the control subjects (both of the P values = 0.004). Discussion: In our study, we conclude that two of the most crucial underlying pathogenic mechanisms of MS, inflammation and oxidative stress, may be associated with the increased levels of ADA and XO, and an approach of targeting the activity of these two enzymes can be considered in treatment strategies. Furthermore, we also demonstrated that ADA and XO enzymes were elevated even in the remission phases of RRMS, reflecting the continuity of inflammation through the whole course of RRMS. Thus, in this disease, which is thought to have a dynamic process, the importance of continuous immunomodulatory treatment is emphasized once again.Öğe The Association of Plasminogen Activator Inhibitor Type 1 (PAI-1) Level and PAI-1 4G/5G Gene Polymorphism with the Formation and the Grade of Endometrial Cancer(SPRINGER/PLENUM PUBLISHERS, 2017) Yildirim, Malik Ejder; Karakus, Savas; Kurtulgan, Hande Kucuk; Kilicgun, Hasan; Ersan, Serpil; Bakir, SevtapPlasminogen activator inhibitor type 1 (PAI-1) is a serine protease inhibitor (Serpine 1), and it inhibits both tissue plasminogen activator and urokinase plasminogen activator which are important in fibrinolysis. We aimed to find whether there is a possible association between PAI-1 level, PAI-1 4G/5G polymorphism, and endometrial cancer. PAI-1 levels in peripheral blood were determined in 82 patients with endometrial carcinoma and 76 female healthy controls using an enzyme-linked immunoassay (ELISA). Then, the genomic DNA was extracted and screened by reverse hybridization procedure (Strip assay) to detect PAI 1 4G/5G polymorphism. The levels of PAI-1 in the patients were higher statistically in comparison to controls (P < 0.001). The distribution of PAI-1 4G/5G polymorphism was quite different between patients and controls (P = 0.008), and 4G allelic frequency was significantly higher in the patients of endometrial cancer than in controls (P = 0.026). We found significant difference between Grade 1 and Grade 2+3 patients in terms of the PAI-1 levels (P = 0.047). There was no association between PAI-1 4G/5G polymorphism and the grades of endometrial cancer (P = 0.993). Our data suggest that the level of PAI-1 and PAI-1 4G/5G gene polymorphism are effective in the formation of endometrial cancer. PAI-1 levels are also associated with the grades of endometrial cancer.Öğe Asymmetric dimethylarginine and M30 concentrations in heart failure(DERMAN MEDICAL PUBL, 2017) Dogan, Halef Okan; Beton, Osman; Ulger, Dilara; Ersan, Serpil; Bakir, DenizAim: Apoptosis has been implicated in the development of various cardiovascular diseases. Caspase-cleaved cytokeratin 18 (M30) is released during apoptotic cell death. The concentrations of M30 and the correlation with asymmetric dimethylarginine (ADMA) in heart failure (HF) are not known. The objectives of this study were to determine the possible association between M30 and ADMA and the potential use of M30 as an apoptotic marker in patients with HF. Material and Method: In this study M30 and ADMA concentrations were evaluated in 30 patients with heart failure and 30 healthy control subjects. Results: Increased M30 (p-0.01) and ADMA (p - 0.012) concentrations were found in the patients and a positive correlation was determined between ADMA and M30 in the patient group (p < 0.001, r- 0.627). No correlation was determined between M30, N terminal pro brain natriuretic peptide (NT-pro-BNP). and ejection fraction. Discussion: These results demonstrate that M30 can be used as a novel apoptotic serum marker in patients with heart failure. The apoptotic cascade activated by increased ADMA concentrations can be considered to contribute to the molecular mechanism of HF.Öğe Changes in arginine metabolism in advanced Alzheimer?s patients: Experimental and theoretical analyses(Elsevier, 2023) Saria, Ismail; Ersan, Serpil; Ozmen, Esma; Ayan, Durmus; Ersan, Erdal; Berisha, Avni; Kayae, SavasLimited data obtained in studies conducted in recent years suggest that changes in arginine metabolism may be associated with the pathogenesis of Alzheimer's disease (AD). However, the underlying mecha-nisms of this pathway's effects on the disease are not clear and there are conflicting data. Therefore, in this study, we aimed to determine the levels of L-arginine and its important metabolites and enzymes involved in the pathway in advanced AD patients to examine the change in L-arginine metabolism as inclusively as possible.Serum and plasma samples were obtained from 51 patients diagnosed with advanced AD and 30 volunteer controls. Arginase, Ornithine Decarboxylase (ODC), Arginine Decarboxylase (ADC), and Agmati-nase levels in serum samples were determined by enzyme-linked immunosorbent assay (ELISA) and, L- arginine, Ornithine and nitric oxide (NO) levels were determined by colorimetric method. Agmatine levels were measured by high-performance liquid chromatography in the plasma samples of the study groups. Furthermore, in silico molecular docking studies were performed to get preliminary knowledge about the binding interactions of the agmatine with various targets such as AChE, butyrylcholinesterase (BuChE), BACE-1 and tau protein kinase 1 which play an important role in AD pathogenesis.Agmatine and L-arginine levels were found to be significantly lower in the patient group than in the control group. Milder but not statistically significant reductions were observed in all other param-eters we measured involved in L-arginine metabolism. Furthermore, NO levels were found to be sig-nificantly lower in men with advanced AD patients than in control men. It has been analyzed that agmatine ligand interacts effectively with the studied proteins which play an important role in AD pathogenesis; these interactions were significant and, based on the docking score, occurred in the fol-lowing order: butyrylcholinesterase (PDB id: 1P0I) > Human acetylcholinesterase > Human tau-protein kinase I.In conclusion, in advanced AD patients, the activity of the L-arginine pathway decreased in gen-eral, especially agmatine formation, and this may be due to the decrease in L-arginine levels. Therefore, arginine de novo synthesis may be decreased in advanced AD patients. Furthermore, according to the MolDock binding score, agmatine ligand has a high binding affinity for proteins involved in AD manage-ment and/or pathogenesis. Therefore, agmatine may play a role in the pathogenesis of AD by inhibit-ing the activity of these proteins. However, additional comprehensive studies are needed to clarify these thoughts.(c) 2023 Elsevier B.V. All rights reserved.Öğe Determination of levels of oxidative stress and nitrosative stress in patients with epilepsy(Elsevier, 2020) Ersan, Serpil; Cigdem, Burhanettin; Bakir, Deniz; Dogan, H. OkanBackground: Epilepsy is one of the most common neurological diseases. The underlying pathophysiological mechanisms in epilepsy are still unknown. Oxidative stress is believed to be one of the factors involved in the pathogenesis of epileptogenesis. In various pathophysiological conditions, reactive nitrogen species (RNS) such as nitrogen and peroxynitrite are produced and these RNSs can bind to free nucleosides and nucleotides or to nucleosides and nucleotides existing in the DNA/RNA structure. 8-Nitroguanine (8-NG) is a typical DNA nucleobase product of nitrosative damage generated by RNS. It has been proposed that F2-isoprostanes, in particular 8-iso-Prostaglandin F2a (8-isoPGF2a), are specific, reliable and non-invasive biomarkers of lipid peroxidation in vivo. In the present study, we compared the levels of lipid oxidative stress biomarker 8-isoPGF2a and nitrosative stress DNA biomarker 8-NG in patients with epilepsy undergoing antiepileptic drug (AEDs) treatment and with those in healthy participants. Methods: The present study comprised 90 patients aged between 17 and 53 who were admitted to the Neurology Clinic of Cumhuriyet University and diagnosed with epilepsy. The patients were assigned into the intervention (n = 45) and control (n = 45) groups. Of the participants in the intervention group, 37.7% (n = 17) were treated with levetiracetam (LEV), 33.3% (n = 15) with valproic acid (VA) and 29% (n = 13) with carbamazepine. Serum 8-iso-PGF2a and 8-NG levels of the participants in the intervention and control groups were determined by ELISA. Results: There was no significant difference between the medication (LEV, VA, Carbamazepine) used by the participants and their 8-iso-PGF2a and 8-NG levels (p> 0.05). However, 8-iso-PGF2a and 8-NG were significantly higher in the participants in the intervention than in the participants in the control group (p< 0.001). Conclusion: Our study demonstrated that there was an increase in oxidative and nitrosative stres markers in patients with epilepsy. There was no significant difference between the 8-iso-PGF2a and 8-NG levels of the participants taking three different AEDs.Öğe Effects of agmatine, glutamate, arginine, and nitric oxide on executive functions in children with attention deficit hyperactivity disorder(Springer Wien, 2020) Sari, Seda Aybuke; Ulger, Dilara; Ersan, Serpil; Bakir, Deniz; Uzun Cicek, Ayla; Ismailoglu, FiratIn this study, we aimed to investigate the effects of agmatine, nitric oxide (NO), arginine, and glutamate, which are the metabolites in the polyamine pathway, on the performance of executive functions (EF) in attention deficit hyperactivity disorder (ADHD). The ADHD group included 35 treatment-naive children (6-14 years old) who were ewly diagnosed with ADHD. The control group consisted of 35 healthy children with the same age and sex, having no previous psychiatric disorders. In the study groups, Stroop test (ST) and trail making test (TMT) were used to monitor EF, and blood samples were collected to measure agmatine with ultra-high-performance liquid chromatography and NO, glutamate, and arginine with enzyme-linked immunosorbent assay (ELISA). The EFs were significantly impaired in the ADHD group. The agmatine and arginine levels of the ADHD group were significantly higher than their peers. The NO and glutamate levels were also higher in the ADHD group compared to the control group, but these differences did not reach statistical significance. Children with ADHD had more difficulties during EF tasks compared to healthy children. The elevated NO and glutamate levels may be related with the impairment during EF tasks. Therefore, agmatine and arginine may increase to improve EF tasks through its inhibitory effect on the synthesis of NO and glutamate. Further studies are needed about polyamine pathway molecules to shed light on the pathophysiology of ADHD.Öğe Effects of cement dust exposure on malonyldialdehyde levels and catalaze activities in red blood cells(ASIAN JOURNAL OF CHEMISTRY, 2008) Nur, Naim; Yilmaz, Abduelkerim; Ersan, Serpil; Gueler, Nuran; Suemer, Haldun; Bakir, SevtapThis study was designed to investigate the plasma oxidant and antioxidant status in both cement plant workers and control subjects. Malonyldialdehyde (MDA) levels and catalaze (CAT) activity were investigated for 42 cement plant workers and the results were compared to 44 control subjects. The MDA levels in erythrocytes were significantly higher in the cement plant workers than in the control group. The CAT activity in the erythrocytes of workers occupationally exposed to cement dust was significantly lower than in the erythrocytes of control group. Occupational exposure to cement dust increased MDA but decreased antioxidant levels in cement plant workers.Öğe Examination of free radical metabolism and antioxidant defence system elements in patients with obsessive-compulsive disorder(PERGAMON-ELSEVIER SCIENCE LTD, 2006) Ersan, Serpil; Bakir, Sevtap; Ersan, E. Erdal; Dogan, OrhanFree radicals and oxidative stress are involved in the pathogenesis of various diseases. Malondialdehyde (MDA) is a natural product of lipid peroxidation in all mammalian cells. Vitamins C and E are nonenzymatic antioxidant structures. Our study investigated the role of free radicals in the obsessive-compulsive disorder (OCD). The participants were 30 patients with OCD that were drug-free at least for a month and a control group of 30 healthy subjects, matched with respect to age and sex. In both groups, the levels of erythrocyte malondialdehyde and the plasma vitamin C and E concentrations were measured. The levels of malondialdehyde were significantly higher in the patient group than in the control group (p < .01). The levels of plasma vitamin E were significantly lower in the patient than in the control group (p < .02). Although our patient group had slightly lower concentrations of plasma vitamin C compared to our control group, the difference between these two groups was not statistically significant. There was a significant correlation between increasing malondialdehyde levels and decreasing vitamin E concentrations. This study shows the presence of a significant relationship of OCD and oxidative stress, and consequently, an involvement of free radicals and of the antioxidant defence. Biochemical studies may contribute to the understanding of OCD and its treatment. (c) 2006 Elsevier Inc. All rights reserved.Öğe Increased Irisin Concentrations in Patients with Crimean-Congo Hemorrhagic Fever(NATL INST INFECTIOUS DISEASES, 2017) Buyuktuna, Seyit Ali; Dogan, Halef Okan; Bakir, Deniz; Ersan, Serpil; Koc, Derya; Bakir, SevtapCrimean -Congo Hemorrhagic Fever (CCHF) is a life-threatening viral infection. The pathogenesis of the disease is not well understood. The aim of this study was to determine the change in irisin concentrations in patients with CCHF. The study included a total of 30 patients with CCHF and 30 control participants. Irisin concentrations were determined using a commercial ELISA kit. Median irisin concentrations were 9.03 (5.81-12.22) mu g/mL and 4.2 (3.39-7.62) mu g/mL, respectively, in each group. There was no correlation between irisin and disease severity. Any correlations between irisin, and lactate dehydrogenase (LDH), international normalization ratio (INR), Alanine aminotransferase (ALT), aspartate aminotransferase (AST), platelets, activated partial thromboplastin time (aPTT), D-dimer and hemoglobin, were also investigated. There were statistically significant positive correlations between the values of irisin, and platelet (p = 0.005, r: 0.369), ALT (p = 0.049, r: 0.261), INR (p = 0.006, r: 0.359) and aPTT values (p = 0.002, r: 0.405). A negative correlation was also found between the values of irisin and LDH (p = 0.008, r: 0.348). No correlations were determined between the values of irisin, and AST, hemoglobin and D-dimer. These results suggest that irisin may have a role in CCHF.Öğe Oxidative, nitrosative and glycosative stress levels in Crimean-Congo hemorrhagic fever disease(Bayrakol Medical Publisher, 2020) Ersan, Serpil; Bakir, Sevtap; Engin, Aynur; Bakir, MehmetAim: This study aimed to investigate Oxidative/Nitrosative/Glycosative stress (OS/NS/GS) biomarkers levels in CCHF disease, their levels in the course of the disease, and to benefit from the results obtained in the pathogenesis and treatment of the disease. Material and Methods: In the study, serum OS, NS, and GS biomarkers levels of the participants in the CCHF (n = 60) and control (n = 35) groups were compared. In addition, the participants with CCHF were classified as mild, moderate, and severe infection subgroups according to the Severity Grading Score (SGS). A commercial enzyme-linked immunosorbent test kit was used to measure the levels of 8-OHdG, 3-NT, 8-NG, NO, CML, 8-iso-PGF2a in serum samples obtained from the participants in the CCHF and control groups. MDA levels were measured in serum samples by a spectrophotometric method. Total Antioxidant Status and Total Oxidant Status levels were determined using commercial kits. Results: On the whole, the mean OS/NS/GS biomarkers levels in the participants in the CCHF group were significantly higher than were those in the control group (p <0.005). Accordingly, it was found that in the participants with CCHF, as the severity of the disease increased so did the biomarker levels. Discussion: Consequently, in addition to routine laboratory tests, the presence of unbalanced OS/NS/GS in CCHF should be taken into account in the followup of patients. Considering that the main factor in CCHF treatment is supportive therapy, adding antioxidant agents to the treatment can contribute to the improvement of the prognosis.Öğe Periodontal status and cytoplasmic enzyme activities in gingival crevicular fluid of type 2 diabetic and/or obese patients with chronic periodontitis.(2006) Gursoy, Ulvi Kahraman; Marakoglu, Ismail; Ersan, SerpilType 2 diabetes mellitus and obesity are the most common nutritional disorders in developed and developing countries. Increased prevalence of periodontal disease is a well-known complication of type 2 diabetes mellitus (DM). As obesity is generally the first step toward type 2 diabetes mellitus, it is possible to find exacerbated periodontal disease in obese patients, also. The purpose of this cross-sectional study was to investigate the periodontal status and aspartate aminotransferase and lactate dehydrogenase enzyme activities in gingival crevicular fluid (GCF) of type 2 diabetic and/or obese chronic periodontitis patients. A total of 39 chronic periodontitis patients participated in the study. The study population was divided into four groups according to body mass index and type 2 DM status: 1) type 2 DM obese patients, n = 8; 2) type 2 DM patients, n = 12; 3) obese patients, n = 8; 4) systemically healthy control group, n = 11. Enzyme activities in gingival crevicular fluid and periodontal status were evaluated. No significant differences in age, gingival index, plaque index, aspartate aminotransferase and lactate dehydrogenase enzyme activities were observed, but probing depths were significantly higher in the DM groups than in the control group. Obesity did not seem to be a significant factor in any parameters evaluated. The present study showed increased probing depth values for the diabetic groups but failed to show any significant relation between obesity and enzyme activity or periodontal status. However, the slightly increased probing depth values in the obese groups might be a clue to an impaired immune response and predisposition to periodontitis in that patient group.Öğe Presepsin Levels of Patients with Crimean-Congo Hemorrhagic Fever(NATL INST INFECTIOUS DISEASES, 2016) Demirpence, Ozlem; Dogan, Halef Okan; Ersan, Serpil; Sahin, Mehtap; Sahin, Hasan; Bakir, MehmetLevels of presepsin (a soluble cluster of differentiation subtype 14 [CD14]) are thought to increase in cases of bacterial infection. CD14 has also been found to play a role in the pathogenesis of various viral diseases. Crimean-Congo hemorrhagic fever (CCHF) is a zoonotic arboviral infection. Our study focuses on presepsin levels as a biomarker for CCHF. Serum presepsin levels in a CCHF group (n = 59) and control group (n = 28) were compared. Patients with CCHF were classified according to severity grading score as having mild, moderate, or severe infection and were allocated to corresponding subgroups (groups 1, 2, and 3, respectively). Presepsin levels were measured in serum samples by using a commercial enzyme-linked immunosorbent assay kit. The mean presepsin levels in the CCHF group as a whole and the healthy group were found to be significantly different (1,499.46 +/- 411.96 pg/ml and 430.68 +/- 61.21 pg/ml, respectively). The mean presepsin levels of the CCHF subgroups (1, 2 and 3) and the healthy group were also found to be significantly different (1,204.53 +/- 371.18, 1,464.21 +/- 338.37, 2,007.36 +/- 82.18, and 430.68 +/- 61.21 pg/ml, respectively) (p < 0.05). We also found that as the severity of the disease increased, the presepsin level also increased. We postulate that the presepsin levels could be used as a supportive biomarker for diagnosis and follow-up of the disease.Öğe Prostaglandin metabolite levels in patients with schizophrenia(CUMHURIYET UNIV TIP FAK PSIKIYATRI ANABILIM DALI, 2011) Ersan, E. Erdal; Ersan, Serpil; Dogan, OrhanObjective: Schizophrenia is a neuropsychiatric disease which affects individuals, family members and society. Although many studies have been carried out on the etiopathogenesis of schizophrenia, the condition is not entirely understood. In this study, it has been aimed to measure prostaglandin (PG) metabolite levels in patients with schizophrenia. Methods: The present study measured prostaglandin metabolite levels in 50 schizophrenia patients and 50 healthy volunteers who were matched for age, sex and smoking is similar in terms of, at least two years of patient. Measurements were also taken of 13,14-dihydro-15-keto-PGF(2 alpha) (PGFM) and 13,14-dihydro-15-keto PGE(2) (PGEM). The measurements were performed using High Performance Liquid Chromatography (HPLC) which is a more sensitive device. Statistical analysis of the data was performed using Student's t Test, Chi Square Test, Kruskal-Wallis Test and Mann-Whitney Test. Findings: In the patient group, the PGEM level (0.0807+/-0.014, t=3.490, p=0.001) and the PGFM level (0.0613+/-0.0128, t=-7.486, p=0.000) were both significantly lower than in the control group (PGEM 0.0907+/-0.0146; PGFM 0.0793+/-0.0113). Discussion: These results suggest that a disorder in phospholipid metabolism may be important in the etiopathogenesis of schizophrenia. (Anatolian Journal of Psychiatry 2011; 12:100-106)Öğe Relationship between chronic obstructive pulmonary disease and telomerase, agmatine, deubiquitinase and sirtuin in the pathway of aging(Bayrakol Medical Publisher, 2023) Kurucay, Deniz; Ozmen, Zeliha Cansel; Seyfikli, Zehra; Ersan, SerpilAim: Chronic Obstructive Pulmonary Disease (COPD) is a progressive disease that has both chronic bronchitis-related changes in airways and differences of emphysema in the lung parenchyma. Aging is a complex and irreversible process involving cells, tissues, organs and systems resulting from numerous endogenous and exogenous factors. Agmatine, telomerase, deubiquitinase and sirtuin molecules appear during aging process. A better understanding of the role of these molecules in aging process may lead to advancing therapies for COPD and comorbidities.In the present study, it was aimed to investigate whether there was a relationship between COPD, a chronic progressive disease, and agmatine, telomerase, sirtuin and deubiquitinase, which are molecules involved in the aging pathway.Material and Methods: Thirty-five patients with COPD and 35 healthy persons without chronic diseases participated in this study. Serum agmatine levels were measured by a fluorescence detector using a high-performance liquid chromatography method (HPLC). Serum Sirtuin, deuibiquitinase and telomerase levels were performed using the ELISA method.Results: A result of the statical analysis in the terms of the deubiquitinase showed no differences between patients and control groups (p>0.05). In the patient group, sirtuin values were lower than in controls (P<005). Telomerase and agmatine values were higher (P<0.05).Discussion: As a result, the increase in telomerase activity may be to prolong the telomere shortening in COPD patients. The increase in agmatine may also be caused by depression and hypoxia in COPD. Decreased sirtuin in COPD can be explained by the increase in aging and inflammation.Öğe Role of MTHFR gene polymorphisms, serum tissue inhibitor of metalloproteinases-1, thymus chemokine-1 and thrombospondin-1 in endometrial cancer(E-CENTURY PUBLISHING CORP, 2016) Yildirim, Malik Ejder; Kilicgun, Hasan; Kurtulgan, Hande Kucuk; Karakus, Savas; Ersan, Serpil; Bakir, Sevtap; Sezgin, IlhanThe aim of this study was to evaluate the relationship between the MTHFR gene variants (677 C -> T and 1298 A -> C), serum tissue metalloproteinases inhibitor (TIMP-1), thrombospondin-1 (TSP-1), thymus chemokine-1 (TCK-1) levels and endometrial cancer. Sixty women were chosen from endometrial cancer patients and fifty-six women without any systemic disease were included as the control group. MTHFR C677T and A1298C MTHFR polymorphisms and their allele frequencies were evaluated with strip assay (Reverse hybridization method). Serum tissue inhibitor of metalloproteinases-1, thymus chemokine-1, and thrombospondin-1 levels were measured with the enzyme-linked immunosorbent assay (ELISA). Genotypic distribution and allelic frequencies of MTHFR C677T polymorphism were not associated with endometrial cancer (P>0.05). But, genotypic distribution and allelic frequencies of MTHFR A1298C polymorphism were strongly associated with endometrial cancer (P = 0.047 and P = 0.024). On the other hand, tissue inhibitor of metalloproteinases-1, thrombospondin-1, and thymus chemokine-1 levels were strongly associated with endometrial cancer (P = 0.001, P = 0.02, and P = 0.001 respectively). These results indicate that genotypic distribution and allelic frequencies of MTHFR A1298C polymorphism, tissue inhibitor of metalloproteinases-1, thrombospondin-1 and thymus chemokine-1 may be the prognostic markers in endometrial carcinoma.Öğe Serum selenium and plasma malondialdehyde levels and antioxidant enzyme activities in patients with obsessive-compulsive disorder(PERGAMON-ELSEVIER SCIENCE LTD, 2009) Ozdemir, Ercan; Cetinkaya, Sevil; Ersan, Serpil; Kucukosman, Seda; Ersan, E. ErdalThere is mounting evidence indicating that reactive free radical species are involved in initiation and development of many different forms of human pathologies including psychiatric disorders. In the present study, we aimed to determine whether serum selenium (Se), antioxidant enzyme (glutathione peroxidase, GSH-Px, superoxide dismutase, SOD, and catalase, CAT) activities, and plasma malondialdehyde (MDA) levels, a product of lipid peroxidation, were associated with obsessive-compulsive disorder (OCD). The participants were 28 patients with OCD that were drug-free at least for a month and a control group (n = 28) of healthy subjects, matched with respect to age and sex. In both groups, the levels of the erythrocyte MDA, GSH-Px. SOD, Se, and the CAT were measured. The levels of MDA and SOD were statistically significantly higher (p<0.01, p<0.05 respectively) in patients than controls. The activities of CAT, GSH-Px, and serum Se levels were statistically significantly lower (p<0.0001, p<0.001, and p<0.001 respectively) in patients than controls. There was a positive correlation in patients between plasma GSH-Px activity and Se concentration (r=52, p=0.001). However, in patients with OCD, CAT and SOD activities were significantly and negatively correlated with MDA levels (r=-0.45, p=0.017 for CAT and r=-0.54, p=0.020 for SOD). The study shows the presence of a significant relationship of OCD and oxidative stress, and consequently, an involvement of free radicals and of the antioxidant defence. (c) 2008 Elsevier All rights reserved.Öğe The impact of Dicer, Drosha, and Exportin-5 levels in polycystic ovary syndrome (PCOS) diagnosis and phenotyping(Via Medica, 2022) Duman, Gulhan; Sariakcali, Baris; Ersan, Serpil; Bakir, SevtapIntroduction: Polycystic ovary syndrome (PCOS) is a very common heterogeneous endocrine and gynaecological disease in reproductive women. Early identification and treatment of patients are necessary to prevent future cardiometabolic and reproductive complications. In our study, we aimed to investigate whether Drosha, Exportin-5 (XPO5), and Dicer, which are involved in miRNA formation, are useful markers in the diagnosis of the disease. Material and methods: Patients who presented to our clinic with complaints such as menstrual irregularity, hirsutism, and acne were diagnosed with polycystic ovary after excluding other possible diagnoses, and if they meet two-thirds of the Rotterdam diagnostic criteria, they were included in the study. Thirty patients with polycystic ovaries and 35 healthy controls were included in this study. Results: The mean values of XPO5, Drosha, and Dicer markers were significantly higher in the PCOS group when compared with the control group. With an XPO5 value > 1.70, we found the PCOS with 94% probability, 86.7% sensitivity, and 91.4% specificity. Moreover, if the Drosha value was > 0.166, it was expected that the patient would be diagnosed as PCOS with a probability of 75%, with 66.7% sensitivity and 71.4% specifidty. A statistically significant cut-off value could not be obtained for Dicer. Conclusions: In our study, the levels of all three markers were found to be significantly higher in the PCOS group compared to the control group. It suggests that they can be used in the early diagnosis of PCOS patients without full-blown disease. However, this preliminary study should be supported by larger-scale studies.Öğe Thiol Disulfide Homeostasis in Schizophrenic Patients Using Atypical Antipsychotic Drugs(KOREAN COLL NEUROPSYCHOPHARMACOLOGY, 2018) Dogan, Halef Okan; Ersan, Etem Erdal; Aydin, Huseyin; Erdogan, Serpil; Ersan, Serpil; Alisik, Murat; Bakir, Sevtap; Erel, Ozcan; Koc, DeryaObjective: Schizophrenia is a severe, debilitating mental disorder characterized by behavioral abnormalities. Although several studies have investigated the role of oxidative stress and the effects of antipsychotic drugs on oxidative markers in schizophrenia, adequate information is not available on these issues. The aim of this study is to determine the changes in oxidative status and thiol disulfide homeostasis in schizophrenic patients using atypical antipsychotic drugs. Methods: Thirteen schizophrenic patients using atypical antipsychotic drugs and 30 healthy controls were included this study. The concentrations of total oxidant status (TOS), total antioxidant status (TAS), native thiol, total thiol, and disulfide levels were determined in the study population. Results: The TAS (p=0.001), total thiol, and native thiol levels (p<0.001) were higher in the patients compared to the controls, whereas the TOS and disulfide levels were lower in the patients than in the controls (p<0.001). Conclusion: These results may suggest that atypical antipsychotic drugs have a useful therapeutic effect by reducing oxidative stress via the inhibition of the formation of disulfide bonds. The study population number was one of the limitations of this study. Therefore, further studies are needed to establish the association between thiol disulfide homeostasis in schizophrenic patients using atypical antipsychotic drugs.
