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    Assessment of antimicrobial activity and In Vitro wound healing potential of ZnO nanoparticles synthesized with Capparis spinosa extract
    (Springer Heidelberg, 2023) Sezen, Selma; Ertugrul, Muhammed Sait; Balpinar, Ozge; Bayram, Cemil; Ozkaraca, Mustafa; Okkay, Irmak Ferah; Hacimuftuoglu, Ahmet
    Agents that will accelerate wound healing maintain their clinical importance in all aspects. The aim of this study is to determine the antimicrobial activity of zinc oxide nanoparticles (ZnO NPs) ZnO nanoparticles obtained by green synthesis from Capparis spinosa L. extract and their effect on in vitro wound healing. ZnO NPs were synthesized and characterized using Capparis spinosa L. extract. ZnO NPs were tested against nine ATCC-coded pathogen strains to determine antimicrobial activity. The effects of different doses (0.0390625-20 mu g/mL) of NPs on cell viability were determined by MTT assay. The effect of ZnO NPs doses (0.0390625 mu g/mL, 0.078125 mu g/mL, 0.15625 mu g/mL, 0.3125 mu g/mL, 0.625 mu g/mL, 1.25 mu g/mL) that increase proliferation and migration on wound healing was investigated in an in vitro wound experiment. Cell culture medium obtained from the in vitro wound assay was used for biochemical analysis, and plate alcohol-fixed cells were used for immunohistochemical staining. It was determined that NPs formed an inhibition zone against the tested Gram-positive bacteria. The ZnO NPs doses determined in the MTT test provided faster wound closure in in-vitro conditions compared to the DMSO group. Biochemical analyses showed that inflammation and oxidative status decreased, while antioxidant levels increased in ZnO NPs groups. Immunohistochemical analyses showed increased expression levels of Bek/FGFR2, IGF, and TGF-beta associated with wound healing. The findings reveal the antimicrobial effect of ZnO nanoparticles obtained using Capparis spinosa L. extract in vitro and their potential applications in wound healing.
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    The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L.
    (Marmara Univ, 2023) Sezen, Selma; Ertugrul, Muhammed Sait; Bayram, Cemil; Ozkaraca, Mustafa; Koc, Taha Yasin; Demir, Abdussamed Yasin; Gulluce, Medine
    Products from Mentha species are widely used in medical applications, including wound healing, due to the bioactive compounds they may contain. However, data on the effect of Mentha longifolia L. on wound healing are limited. This study investigated the antimicrobial and wound healing-promoting effects of Mentha longifolia L. essential oil using in vitro methods. The chemical compositions of the essential oil were identified using gas chromatography/mass spectrometry, while the agar well diffusion and disk diffusion methods were used to determine antimicrobial activity against pathogenic strains. A scratch wound healing assay was performed following determination of the cytotoxic dose in human fibroblast cell lines. The media were used for biochemical analysis 48 h after the in vitro wound model. Immunohistochemical staining was applied to determine the contribution of the essential oil to wound healing. The essential oil exhibited varying levels of antimicrobial activity against the tested pathogens and increased cell viability. All doses in the scratch wound healing assay promoted wound closure in a shorter time than in the control group. TAC levels were higher in the treated groups than in the control group, while TOS, IL-6, and TNF-alpha levels were lower. Levels of expression of FGF 2, IGF, and TGF-beta were higher in the treated groups than in the control group at increasing doses. The essential oil of Mentha longifolia L. exhibited antimicrobial effects and improved wound healing at doses of 5 mu g/mL and 10 mu g/mL.