Yazar "Eruygur, Nuraniye" seçeneğine göre listele

Listeleniyor 1 - 20 / 39
  • Küçük Resim Yok
    Öğe
  • Küçük Resim Yok
    Öğe
    Anticancer Activity of Water and Methanol Extracts of Hypericum scabrum L. on Different Cancer Cell Lines
    (Horticulture and Forestry Society from Transylvania, 2019) Guclu, Gulsen; Tas, Ayca; Tulimat, Manhal; Eruygur, Nuraniye; Silig, Yavuz
    Hypericum scabrum L. is an endemic medicinal plant with antimicrobial, anticancer and anti-inflammatory, antiviral effect. In this study, we aimed to investigate the cytotoxic effect of water and methanol extracts on different cancer cell lines such as osteosarcoma cancer, cervical cancer and prostate cancer. The data showed that the methanol extract had a highly effective cytotoxic effect on all three cell lines. Although water extract caused less cell loss than methanol extract, it was found that the cells were inhibited in all three cell lines. DU-145, which is the prostate cell line, was more effective in both extracts. As a result, the water and methanol extract of Hypericum scabrum L. have an anticancer effect on Saos-2, HeLa and DU-145 cells. There is a need for further and comprehensive studies by isolating the main active ingredient of this plant which is a candidate for drug of cancer in the future. Our study is expected to lead the studies in this direction. © 2019. All Rights Reserved.
  • Küçük Resim Yok
    Öğe
    Anticancer, Antioxidant, Antimicrobial and Enzyme Inhibitory Activities of Inula aucheriana
    (Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Guclu, Gulsen; Ergul, Merve; Ucar, Esra; Eruygur, Nuraniye; Atas, Mehmet; Akpulat, Huseyin Askin
    The Inula aucheriana, of the Asteraceae family, is widespread in Turkey. The aim of this study was to investigate different biological properties and thereby reveal the pharmacological potential of this plant, which is already known to be used in the treatment of various diseases,. Following the preparation of 80% ethanol extract from I. aucheriana, qualitative and quantitative methods were used to investigate the chemical composition (Q-TOF analysis), antioxidant (spectrophotometric analysis), inhibitory enzyme (Ellman's method), antimicrobial (MIC concentration value), and anti-cancer (XTT analysis) activities. The results showed that 80% ethanol extract from I. aucheriana was a potent antioxidant, with anti-cancer and enzyme inhibitor effects. In the chemical composition analysis, the primary compound of the extract was determined to be luteolin (32.55%). I. aucheriana extract was seen to have AChE and BChE inhibition, , and when compared with the reference drug, the extract was determined to have an inhibitory effect an enzyme called alpha-glucosidase. Besides, relatively high tyrosinase enzyme inhibition was also detected. The extract significantly showed antiproliferative activity on the MDA-MB-231 cells at 0.0625 mg/mL and higher concentrations,for 24 hours in a a dose-dependent manner.This study is the first to evaluate enzyme inhibitory effect and antioxidant activity of I. aucheriana.
  • Küçük Resim Yok
    Öğe
    Bioassay-guided isolation of wound healing active compounds from Echium species growing in Turkey
    (ELSEVIER IRELAND LTD, 2016) Eruygur, Nuraniye; Yilmaz, Gulderen; Kutsal, Osman; Yucel, Gozde; Ustun, Osman
    Ethnopharmacological relevance: The roots and root barks of Echium sp. have been used to treat ulcers, burns and wounds in traditional Turkish medicine. Aim of the study: On the basis of them traditional use and literature references, four Echium species were selected for evaluation of them wound healing potential. Isolation of active component(s) from the active extracts through the bioassay guided fractionation procedures. Material and methods: In vivo the wound healing activity of the plants was evaluated by linear incision experimental models. The chloroform extract of Echium italicum L. was fractionated by successive chromatographic techniques. Wound healing activity of each fraction was investigated following the bioassay-guided fractionation procedures. Moreover, the tissue samples of isolated compounds were examined histopathologically. The healing potential was comparatively assessed with a reference ointment Madecassol (R), which contains 1% extract of Centella asiatica. Results: Significant wound healing activity was observed from the ointment prepared with ethanol extract at 1% concentration. The ethanol root extract treated in groups of animals showed a significant increase (37.38%, 40.97% and 35.29% separately for E. italicum L, Echium vulgare Land Echium angustifolium Miller) wound tensile strength in the incision wound model. Subfractions showed significant but reduced wound healing activity on in vivo wound models. Shikonin derivatives "Acetylshikonin", "Deoxyshikonin" and "2-methyl-n-butyrylshikonin+lsovalerylshikonin", were isolated and determined as active components of active final subtraction from E. italicum L. roots. The results of histopathological examination supported the outcome of linear incision wound models. Conclusion: The experimental study revealed that Echium species display remarkable wound healing activity. (C) 2016 Published by Elsevier Ireland Ltd.
  • Küçük Resim Yok
    Öğe
    Biological activities of different plant species belonging to the Asteraceae family
    (Pamukkale University, 2023) Guclu, Gulsen; Inanir, Merve; Ucar, Esra; Eruygur, Nuraniye; Atas, Mehmet; Uskutoğlu, Tansu; Senkal, Belgin Cosge
    Achillea biebersteinii and Anthemis tinctoria, which are widely distributed species of the Asteraceae family, are used in folk medicine in the form of herbal tea or extract in the treatment of many diseases. The aim of this study was to investigate the chemical content, antimicrobial, antioxidant, enzyme inhibitor activities and cytotoxic effects of 80% ethanol extract of these two species and make a comparative analysis. In accordance with the data obtained, the major component of A. biebersteinii was determined as Cyclododecane (14.47%), while that of A. tinctoria was determined as Phytol (23.15%). A. biebersteinii, which showed moderate activity in terms of antimicrobial activity, produced more active inhibition than A. tinctoria did. Both plants showed high levels of antioxidant activity. The total phenol and total flavonoid contents of A. tinctoria were higher than those of A. biebersteinii. It was determined that there was no significant activity when the extracts were compared with galanthamine, which is the reference drug in terms of enzyme inhibitory activity. When the in vitro anticancer activity of human breast cancer cell line was examined, it was determined that A. tinctoria had a cytotoxic effect at high concentrations (IC50;0.82mg/mL), and A. biebersteinii showed strong cytotoxicity at all concentrations (IC50;<0.0625mg/mL). These two plants of the same family were evaluated in terms of many different biological parameters and it was revealed that A. biebersteinii was more active than A. tinctoria. However, in vivo studies are needed to determine whether these plants can be used as phytotherapeutic agents. © 2023 The Author(s).
  • Küçük Resim Yok
    Öğe
    BIOLOGICAL ACTIVITY EVALUATION OF THREE Hypericum L. SPECIES
    (Trakya Univ Balkan Yerlesesi Enstituler Binasi, 2022) Eruygur, Nuraniye; Ozpinar, Necati; Ozpinar, Hulya; Atas, Mehmet; Tekin, Mehmet; Cevik, Ozge
    This study was performed in order to evaluate in vitro antioxidant, anti-Trichomonas vaginalis, antimicrobial and cytotoxic activities of methanol extracts of aerial parts of Hypericum perforatum L., H. thymbrifolium Boiss. & Noe and H. thymopsis Boiss. The antioxidant activity of methanol extracts was tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and iron chelating assays. The total phenol and flavonoid contents were also determined spectrophotometrically. Cytotoxicity and apoptosis were evaluated on MCF-7 breast cancer and PC3 prostate cancer cell line. Antimicrobial activities were evaluated by the microdilution method on the bacterial strains Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853) Escherichia coli (ATCC 25922) and the fungal strain Candida albicans (ATCC 10231). All extracts demonstrated cytotoxic activity on PC3 prostat cancer cell line with a concentration dependent manner, while H. thymopsis extract was the most active against cancer cell growth. The obtained results of the present study revealed that the methanol extract of H. perforatum, H. thymbrifolium and H. thymopsis have significant antioxidant and cytotoxic activities. The results provided the basic research data for further phytochemical and biological activity guided investigations on these species for identication and isolation of potential drug active compounds.
  • Küçük Resim Yok
    Öğe
    Comparative study of in vitro antioxidant, acetylcholinesterase and butyrylcholinesterase activity of alfalfa (Medicago sativa L.) collected during different growth stages
    (DE GRUYTER POLAND SP ZOO, 2018) Eruygur, Nuraniye; Dincel, Burak; Dincel, Nazire Gulsah Kutuk; Ucar, Esra
    Medicago sp. are often consumed as vegetable, salad and herbal tea as a form of fresh leaves and herbs. It is also very important in animal feeding, because it contains high percentage of protein and some important phytochemicals. In addition, traditionally, the leaves or other parts of the plant were used for treatment and preventing of kidney disorders, osteoporosis, anemia, diabetes, ulcer, coronary diseases, some cancers, and menopausal symptoms. In previous reports, it has been demonstrated that this plant has scavenging activity of the free radicals, which are involve in the development of the hypertension, Lschemia, neurodegenerative and rheumatoid diseases. In this study, the antioxidant, enzyme inhibitory activities of M. sativa L. collected in two growing period and phenolic substances that may be responsible for these activities were investigated. In this way, the beneficial effects of the plant will he revealed and a scientific work will be provided for the evaluation of the pharmaceutical and food industry.
  • Küçük Resim Yok
    Öğe
    Comparison of different drying methods for phytochemical quality of stevia (Stevia rebaudiana Bert.)
    (Horticulture and Forestry Society from Transylvania, 2023) Özyiğit, Yaşar; Uçar, Esra; Eruygur, Nuraniye; Ataş, Mehmet; İnanir, Merve; Bal, Halil; Kahrizi, Danial
    This study examined the effects of different drying methods on the chemical content, antioxidant, antibacterial, enzymatic and anticancer activity values of stevia (Stevia rebaudiana Bert.) extract. Stevia leaves were dried using four different methods (in the sun, shade, air conditioner, and oven), and extracts were collected and analyzed. Based on extract content, 2-tetradecyl acrylate was the major ingredient in air-conditioned and oven-dried applications (25.00% and 21.47%, respectively). The same compound was detected in both sun-drying and shade-drying methods, but the content was low. The antioxidant activity values of the samples were evaluated with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging tests. While the best result for DPPH (IC50 value 57.94 ± 0.63 µg/mL) was found in the shade-dried stevia samples, the best result in terms of ABTS test (IC50 value 44.03 ± 1.22 µg/mL) ml) was detected in oven-dried. When the samples were examined in terms of antimicrobial activity, it was seen that extracts of all drying methods were effective against Staphylococcus aureus. However, it was determined that the extracts obtained from plants dried in air conditioning and oven had a stronger effect. The cell viability assay was utilized to assess the antiproliferative effects of extract on L929 and MDA-MB-231 cell lines. The extracts did not significantly affect the L929 cell viability, while MDA-MB-231 remarkably reduced cell viability (sun drying, IC50 = 0.9 mg/mL; oven drying, IC50 = 0.65 mg/mL; shade drying, IC50 = 0.74 mg/mL; air conditioner drying, IC50 = 0.47 mg/mL). In particular, the extract obtained by the air conditioner drying method showed the most prominent cytotoxic effect. The results showed that drying using different methods had an impact on the quality standards of the stevia leaves. © 2023, Horticulture and Forestry Society from Transylvania. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Composition characterization and biological activity study of Thymbra spicata l. var. spicata essential oil
    (2021) Eruygur, Nuraniye; Kocyıgıt, Umit M.; Atas, Mehmet; Cevık, Ozge; Gökalp, Faik; Taslimi, Parham; Gulcın, İlhami
    The current research aimed to determine and report in vitro antioxidant, antimicrobial, antibiofilm, cytotoxic, anti-cholinesterase, and anti-diabetic properties and the stability of the major component of basic oil of Thymbra spicata var. spicata through different phases as theoretically. Essential oil exhibits potential biological activities because of the multiple components it contains.In the current research, the evaluation of Thymbra spicata essential oil antioxidant properties was conducted utilizing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2- azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) radical scavenging activity.Antimicrobial activity was assessed from minimum inhibition concentration (MIC) using the technique of microdilution and cytotoxicity activity was evaluated by MTT assay through MCF-7 and PC3 human cancer cell lines.Consequently, Cytotoxic activity was evaluated by means of MTT assay utilized. The essential oil was detected to have 340 ?g/mL inhibiting influence on the growth of PC3 prostate cancer cells with IC50 value. Also, the T. spicata plant was observed to significantly repress the enzymes, namely acetylcholinesterase (AChE), butyrylcholinesterase (BChE), ?-glycosidase. IC50 values of enzymes were obtained 0.23 ?g/mL for AChE, 1.64 ?g/mL for BChE, 7.78 ?g/mL for ?-glycosidase. It was concluded that this plant may be used for Alzheimer's and diabetes disease.
  • Küçük Resim Yok
    Öğe
    Coronilla parviflora Moench Çiçeklerinin Farklı Ekstraktlarının Biyolojik Aktiviteleri
    (Sivas Cumhuriyet University, 2022) Uçar, Esra; Sarı, Ferda; Eruygur, Nuraniye
    Bu çalışmada, Coronilla parviflora çiçeklerinden elde edilen su ve metanol ekstraktlarının antioksidan aktiviteleri araştırılmıştır. Elde edilen verilere göre metanol ekstraktının antioksidan aktivite değerinin su ekstraktına göre daha güçlü etkiye sahip olduğu görülmüştür. Aynı zamanda metanol ekstraktının TPC (Total Fenol Miktar) değeri de Coronilla parviflora çiçeklerinin su ekstraktından daha yüksek bulunmuştur. Metanol ekstraktının ana bileşeni %20,85 ile “Palmitinik asit”, su ekstraktının ana bileşeni ise “4H-Pyran-4-on, 2,3-dihidro-3,5-” olarak bulundu. dihidroksi-6-metil” ile 23.72 olarak bulunmuştur.
  • Küçük Resim Yok
    Öğe
    Determination of 1-Deoxynojirimycin by a developed and validated HPLC-FLD method and assessment of In-vitro antioxidant, alpha-Amylase and alpha-Glucosidase inhibitory activity in mulberry varieties from Turkey
    (ELSEVIER GMBH, 2019) Eruygur, Nuraniye; Dural, Emrah
    Background: Morus alba and Morus nigra leaves which have been widely used as herbal teas in Anatolian region of Turkey, were extracted twice by 50 mM HCI solution, derivatized with 9-fluorenylmethyl chloroformate and analyzed by reversed phase HPLC equipped with a fluorescence detector. Hypothesis/Purpose: This study was performed to determine the main antidiabetic active compounds 1-deoxynojirimycin by HPLC method and evaluate the in-vitro antioxidant and antidiabetic activity of ethanol extracts prepared from Morus alba L. and Morus nigra leaves. Study Design: A reliable simple, and rapid high-performance liquid chromatographic (HPLC) method for the determination of 1-deoxynojirimycin in M. alba L. and M. nigra leaves with fluorimetric detection after pre-column derivatization with 9-fluorenylmethyl chloroformate was developed. In addition, the chemical composition of ethanol extract of mulberry leaves was analyzed with GC-MS. Methods: Separation and quantitation were performed on C18, 250 x 4.6 mm, 5 mu m analytical column. Mobile phase consisted of acetonitrile and 0.1% acetic acid solution (1:1, v/v) was performed applied to the column 1.0 ml/min flow rate at 26 degrees C. Potential antioxidant activity of ethanol extract of different mulberry varieties were evaluated by DPPH, and ABTS radical scavenging assay as well as total phenol and flavonoid content were determined. In addition, alpha-amylase and alpha-glucosidase activity was determined by 96-well plate method to evaluate the probable antidiabetic potential use of Turkish mulberry leaves. Results: The isocratic HPLC method showed excellent correlation coefficient (r(2) = 0.9985) between 0.3 and 30 mu g/ml calibration points. The method was specific and sensitive with detection and quantification limits of 1.07 and 3.27 ng/ml, respectively. Intraday and interday method precision (n = 5) were < 7.3 (RSD%). Intraday and interday method accuracy (n = 5) were between 3.77 and (-8.35) (RE%). The average method recovery (n = 3) was 102.5%. The results showed that the content of 1-deoxynojirimycin in leaves of Morus alba L. was 0.103% (n = 3), and in leaves of M. nigra L. was 0.102%. 2-hexadecen-1-ol, oleamide, 2-propenoic acid, and cyclododecane were identified as the major compounds by GC-MS in the ethanol extract of mulberry leaves. Conclusion: The obtained robustness values from emission and excitation detection, mobile phase ingredients and flow rates changes showed that method was very strong. This work contributes to the knowledge of antioxidant and antidiabetic properties of Morus species, thus may be provide useful data in evaluation of food products and pharmaceutical preparations produced from Morus species.
  • Küçük Resim Yok
    Öğe
    Determination of biological activity of Tragopogon porrifolius and Polygonum cognatum consumed intensively by people in Sivas
    (Elsevier, 2020) Eruygur, Nuraniye; Ucar, Esra; Atas, Mehmet; Ergul, Merve; Ergul, Mustafa; Sozmen, Fazli
    Objective: This study was aimed to investigate the in vitro antioxidant, antimicrobial, cytotoxicity, and enzyme inhibition activities of Tragopogon porrifolius and Polygonum cognatum which are naturally grown and consumed intensively by people in Sivas, Turkey. Methods: Plant materials were extracted with aqueous ethanol by maceration method. The components of the extracts were determined using the Gas Chromatography Mass Spectrometry. Antimicrobial, cytotoxic and enzyme inhibition activities of the extracts were investigated by micro dilution, XTT assay and 96-micro-well plate methods, respectively. The antioxidant activity evaluated using the DPPH radical scavenging, thiobarbituric acid and reducing power methods. The total phenol and total flavonoid content was also examined. Results: GC-MS analysis revealed the presence of 31 compounds inP. cognatum extract and 29 compounds in T. porrifolius extract. According to the results, T. porrifolius extract showed high level of antioxidant activity in comparison to P. Cognatum extract. T. porrifolius exhibited higher alpha-glucosidase inhibitory activity, and both extract showed strong alpha-amylase inhibition activity compared to reference drug acarbose. T. porrifolius and P. cognatum ethanolic extracts exhibited antimicrobial activity in the concentration range of 0.039-2.5 mg/ml. Both extracts also exhibited significant anticancer effect on MDA-MB-231 breast cancer cells. The IC50 values of T. porrifolius and P. cognatum extracts in MDA-MB-231 cells were determined as 0.0625 mg/mL and 0.053 mg/mL, respectively. Conclusion: Our findings demonstrated that T. porrifolius and P. cognatum ethanolic extracts have promising effect on antioxidant, antimicrobial and cytotoxic activity as well as enzyme inhibition activity, and hence further studies required to identify specific compounds responsible for these activities.
  • Küçük Resim Yok
    Öğe
    Determination of inhibitory activities of enzymes, related to Alzheimer's disease and diabetes mellitus of plane tree (Platanus orientalis L.) extracts and their antioxidant, antimicrobial and anticancer activities
    (C M B ASSOC, 2018) Ucar, Esra; Eruygur, Nuraniye; Atas, Mehmet; Ergul, Merve; Ergul, Mustafa; Sozmen, Fazli
    Plane tree (Platanus orientalis L.) leaves have been employed for centuries in various countries due to their pharmacological value. Therefore, determination of the biological activity of the leaves is of interest. The aim of the study was to evaluate the inhibitory effects against Alzheimer's disease-related enzymes Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE), diabetes mellitus related enzymes alpha-glucosidase and alpha-amylase. The antioxidant, anticancer, and antimicrobial activities of the leaves were also studied. According to the results, both water and methanol extracts of P. orientalis demonstrated more alpha-glucosidase and alpha-amylase inhibition activity than the antidiabetic drug-acarbose at the same concentration level. In addition, extracts showed good inhibition activity against AChE and BuChE. Significant results were obtained regarding antioxidant, anticancer, and antimicrobial activities. These results are very promising especially for the improvement of pharmaceutical formulations to treat various diseases such as age-related diseases, cancer, diabetes etc. and it is necessary to conduct further experiments.
  • Küçük Resim Yok
    Öğe
    The Effect of the Plant Age and Growth Period on the Nutritional Substance, Chlorophyll and Steviol Glycoside Rates in Stevia (Stevia Rebaudiana Bertoni) Leaves
    (TAYLOR & FRANCIS INC, 2018) Ucar, Esra; Ozyigit, Yasar; Eruygur, Nuraniye; Guven, Dilek; Yur, Suleyman; Turgut, Kenan; Ozek, Temel; Kutuk, Nursah
    Stevia (Stevia rebaudiana Bertoni) is a perennial and subshrub herb of the Asteraceae family, which originates from South America. Stevia has long been used as a sweetener and herbal medicine throughout the world. The most important feature of stevia is its sweetness with zero calories. This natural feature of Stevia is of utmost importance to everyone, especially those with diabetes and those who diet. In this study, samples were taken from Stevia rebaudiana plants of different ages (2, 3 and 4 years) in different growth periods (H1: 1 July, H2: 1 August, H3: 1 September and H4: 1 October) and an evaluation was made on the nutritional content values, the chlorophyll and sugar rates found in these samples according to the age and growth period of the plant. It was determined that the age of the plant and the harvesting period had an effect on the macro-micro nutritional elements, and chlorophyll and steviol glycoside rates contained in the stevia plant. In addition, as a result of correlation analysis, significant correlations were determined between the parameters measured in the plant. The results of the study demonstrated that in the cultivation of stevia, the best values were obtained from plants that were 3 years old and harvested in July.
  • Küçük Resim Yok
    Öğe
    Effective and rapid removal of cationic and anionic dyes from aqueous solutions using Elaeagnus angustifolia L. fruits as a biosorbent
    (DESALINATION PUBL, 2019) Oymak, Tulay; Eruygur, Nuraniye
    This study focused on the removal of both cationic and anionic dye from an aqueous solution using a natural product. Evaluation was made of the biosorption of methylene blue (MB) and Indigo carmine (IC) as a basic dye and an acid dye, respectively, from an aqueous solution using the dried fruits of Elaeagnus angustifolia. The experimental parameters such as pH of the solution, initial dye concentration, and contact time, were optimized to achieve quantitative removal. The quantitative biosorption of dyes was achieved as 200 mg L-1 of MB within 10 min at pH 7, 25 mg L-1 of IC within 10 min at pH 2. The maximum biosorption capacity was 344.8 mg g(-1) for MB and 9.7 mg g(-1) for IC at room temperature. The pseudo-first-order model and pseudo-second-order models were used to explain the biosorption kinetics. The second order model best explained MB and IC biosorption onto E. angustifolia. The experimental results showed that the use of E. angustifolia as a biosorbent is fast and effective, thereby indicating that it could be an attractive alternative for the removal of anionic and cationic dyes from dye wastewater.
  • Küçük Resim Yok
    Öğe
    The Effects of different nitrogen doses on antioxidant and antimicrobial activity of Stevia (Stevia rebaudiana Bert.)
    (C M B ASSOC, 2018) Atas, Mehmet; Eruygur, Nuraniye; Ucar, Esra; Ozyigit, Yasar; Turgut, Kenan
    In this study, the efficiency of the different nitrogen doses (0, 5, 10, 15 and 20 kg ha(-1)) on biological activity levels (antioxidant and antimicrobial activity) of Stevia rebaudiana Bert. was investigated. In addition, methanol extracts were obtained by maceration method from different doses of fertilizer applied stevia. The components in methanol extracts of plants were determined by gas chromatography-mass spectrometry (GC-MS) method. Antimicrobial activities of stevia extracts were investigated by microdilution method. The antioxidant activity evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric thiocyanate (FTC), thiobarbituric acid (TBA), reducing power, total phenol content (TPC), and total flavonoid content (TFC) methods. According to the results, the fertilizer doses effects on antimicrobial activity of stevia were not made much difference. But in antioxidant activity, there were some variations in the activity-dependent on fertilizer amount.
  • Küçük Resim Yok
    Öğe
    Estimation of in vitro antioxidant and antimicrobial activity of methanol extracts from Stevia
    (Parlar Scientific Publications, 2019) Eruygur, Nuraniye; Atas, Mehmet; Ucar, Esra; Ozyigit, Yasar; Turgut, Kenan
    In this study, the efficiency of the plant age and growth period on antioxidant and antimicrobial activity of stevia (Stevia rebaudiana Bert.) was investigated. Samples were taken from S. rebaudiana plants of three different ages (2, 3 and 4 years old) and four different growth periods (H1: 1 July, H2: 1 August, H3: 1 September and H4: 1 October). Extracts of sweet herb (S. rebaudiana) were obtained by maceration method with methanol as solvent. The experiments were carried out in completely randomized design with three replications. According to results, the highest antioxidant activity (metal chelating activity of methanol extract from stevia leaf; DPPH Free Radical Scavenging Activity of methanol extract from stevia leaves) was determined from respectively No.2 sample (3-year-old plant harvested in July) and No.1 sample (2-year-old plant harvested in July). The highest antimicrobial and antifungal activity for Escherichia coli and Staphylococcus aureus was determined from No.9, No.10, and No.11 samples (in the order of 3-year-old plant harvested in September, 3-year-old plant harvested in October, and 4-year-old plant harvested in November). All of the stevia samples harvested in different age and different periods were showing antimicrobial effect against Pseudomonas aeruginosa, E. faecalis and Candida albicans. © 2019 Parlar Scientific Publications. All rights reserved.
  • Küçük Resim Yok
    Öğe
    ESTIMATION OF IN VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACTS FROM STEVIA
    (PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2019) Eruygur, Nuraniye; Atas, Mehmet; Ucar, Esra; Ozyigit, Yasar; Turgut, Kenan
    In this study, the efficiency of the plant age and growth period on antioxidant and antimicrobial activity of stevia (Stevia rebaudiana Bert.) was investigated. Samples were taken from S. rebaudiana plants of three different ages (2, 3 and 4 years old) and four different growth periods (H1: 1 July, H2: 1 August, H3: 1 September and H4: 1 October). Extracts of sweet herb (S. rebaudiana) were obtained by maceration method with methanol as solvent. The experiments were carried out in completely randomized design with three replications. According to results, the highest antioxidant activity (metal chelating activity of methanol extract from stevia leaf; DPPH Free Radical Scavenging Activity of methanol extract from stevia leaves) was determined from respectively No.2 sample (3 year-old plant harvested in July) and No.1 sample (2-year-old plant harvested in July). The highest antimicrobial and antifungal activity for Escherichia coli and Staphylococcus aureus was determined from No.9, No.10, and No.11 samples (in the order of 3-year-old plant harvested in September, 3-year old plant harvested in October, and 4-year-old plant harvested in November). All of the stevia samples harvested in different age and different periods were showing antimicrobial effect against Pseudomonas aeruginosa, E. faecalis and Candida albicans.
  • Küçük Resim Yok
    Öğe
    Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant
    (Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Kocyigit, Umit Muhammet; Eruygur, Nuraniye; Atas, Mehmet; Tekin, Mehmet; Taslimi, Parham; Gokalp, Faik; Gulcin, Ilhami
    The research was conducted to separately evaluate and detect the possible in vitro antioxidant, antimicrobial activity of ethanol extracts prepared from aerial parts and roots of Astragalus dumanii and anti-cholinesterase and alpha-glucosidase inhibitory activity from only aerial parts of its The antioxidant capacity was tested by scavenging of DPPH and ABTS free radicals. Compared with the standard antioxidant compound gallic acid; Root and aerial part extract showed lower DPPH radical scavenging activity, however aerial part extract demonstrated higher ABTS radical scavenging activity. The phenolic contents were detected as 5.31 +/- 0.03 and 13.23 +/- 0.05 mg gallic acid equivalent g-1 extract, flavonoid contents were found as 8.26 +/- 0.004 and 7.93 +/- 0.005 mg Qercetin equivalent g-1 extract. In addition, the effects of the extracts obtained from aerial parts of the plant on acetylcholinesterase, butyrylcholinesterase and a-glycosidase enzymes were investigated in vitro and IC50 values were obtained as 1.47, 0.83 and 0.48 mu g mL-1, respectively. When these values were compared with standard substances, it was seen that Astragalus dumanii could be a good enzyme inhibitory agent. Antimicrobial activity of the plant extracts were determined using the microdilution method and the extracts was not observed to have any antimicrobial activities..
  • Küçük Resim Yok
    Öğe
    Evaluation of antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of Hypericum heterophyllum vent., an endemic species in Turkey's Flora
    (Elsevier, 2024) Eruygur, Nuraniye; Ucar, Esra; Tuzun, Burak; Atas, Mehmet; Inanir, Merve; Demirbas, Ahmet; Bal, Halil
    The aim of this study was determined the antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of H. heterophyllum (endemic species in the Flora of Turkey) extracts. H. heterophyllum methanolic extract was used in this study. H. heterophyllum plants collected at the beginning of flowering and during full flowering. P content was determined with calorimetrically in spectrophotometer. K, CA, Mg, Fe, Mn, Zn and Cu was determined with Atomic Absorption and N concentration were determined according to the Kjeldahl distillation method. H. heterophyllum (endemic species in the Flora of Turkey) extract was evaluated for its antioxidant (DPPH, ABTS, total phenol and flavonoid content), antimicrobial (four bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus) and two Candida species (Candida albicans and Candida tropicalis)), enzyme inhibition activity (alpha-glucosidase, alpha-amylase, acetylcholinesterase, butyrylcholinesterase, tyrosinase), and cell viability (XTT assay) capacity. Extracts from the beginning of flowering period showed stronger biological activity. It has been determined that the extracts have antioxidant, antibacterial (against Staphylococcus aureus) anticancer activity (on breast cancer cells), and inhibition effects of BChE and alphaglucosidase. It has been determined that the extracts obtained from H. heterophylum have important pharmacological activities, and the plant exhibits higher activity in the BF period. Hypericum heterophyllum Vent. The activities of the chemicals contained in it against various proteins that are alpha-gly enzyme (PDB ID: 1R47), AChE enzyme (PDB ID: 4M0E), BChE enzyme (PDB ID: 5NN0), alpha-Amylase (PDB ID: 3BAJ), breast cancer protein (PDB ID: 1A52 and 1JNX), were compared. ADME/T analysis of molecules with high activity was performed.